A5076344070- Plant tissue culture and regeneration
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Transgenic Plants and Applications
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Plant Genetic and Mutation Studies
- Metabolomics and Mass Spectrometry Studies
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Microbial bioremediation and biosurfactants
- Potato Plant Research
- Allelopathy and phytotoxic interactions
- Plant Molecular Biology Research
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Gene Regulatory Network Analysis
- Engineering Education and Curriculum Development
- Soybean genetics and cultivation
- Problem and Project Based Learning
- GABA and Rice Research
- Enzyme Structure and Function
University of Illinois Chicago
2023
Iowa State University
2010-2020
Rice University
1991-2001
SRI International
1999
California Institute of Technology
1987-1990
The ability of plants to uptake and transform 2,4,6-trinitrotoluene (TNT) was investigated using the aquatic plant Myriophyllum spicatum, axenic aquaticum, Catharanthus roseus hairy root cultures. Studies demonstrate that Myriophyllum, with or without its periphyton, C. TNT. Low concentrations aminated nitrotoluenes (2-amino-4,6-dinitrotoluene 4-amino-2,6-dinitrotoluene) were observed in extracellular medium tissue extracts. Primary products transformation not identified, mineralization...
Abstract Background The terpenoid indole alkaloid (TIA) pathway leads to the production of pharmaceutically important drugs, such as anticancer compounds vinblastine and vincristine. Unfortunately, these drugs are produced in trace amounts, causing them be very costly. To increase an improved understanding TIA regulatory is needed. Towards this end, transgenic Catharanthus roseus hairy roots that overexpress ORCA2 transcriptional activator were generated characterized. Results...
Late exponential phase hairy root cultures of Catharanthus roseus were elicited with pectinase and jasmonic acid. The effects elicitor concentration exposure time on growth levels several compounds in the indole alkaloid biosynthetic pathway monitored. Pectinase decreased fresh weight to dry ratio roots, while addition acid had no significant effect. Selective yields observed upon elicitors. An increase 150% tabersonine specific yield was 72 units pectinase. Transient studies at same level...
Metabolic flux quantification in plants is instrumental the detailed understanding of metabolism but difficult to perform on a systemic level. Toward this aim, we report development and application computer-aided metabolic analysis tool that enables concurrent evaluation fluxes several primary pathways. Labeling experiments were performed by feeding mixture U-(13)C Suc, naturally abundant Gln developing soybean (Glycine max) embryos. Two-dimensional [(13)C, (1)H] NMR spectra seed storage...
Abstract Hairy root cultures of Catharanthus roseus were established by infection seedlings with Agrobacterium rhizogenes 15834. Approximately 150 transformants from four different. C. cultivars screened for desirable traits in growth and indole alkaloid production. Five hairy clones grew well liquid culture doubling times similar to those reported cell suspensions. Fast growing had morphologies, characterized thin, straight, regular branches thin tips. The levels key alkaloids, ajmalicine,...
In nature, plants generate protective secondary metabolites in response to environmental stresses. Such include terpenoid indole alkaloids (TIAs), which absorb UV-B light and serve putatively protect the plant from harmful radiation. Catharanthus roseus plants, multiple shoot cultures, cell suspension cultures exposed show significant increases production of TIAs, including precursors vinblastine vincristine, have proven effective treatment leukemia lymphoma. Here, effect on C. hairy roots...
The molecular, biochemical, and genetic mechanisms that regulate the complex metabolic network of soybean seed development determine ultimate balance protein, lipid, carbohydrate stored in mature seed. Many genes metabolites participate metabolism are unknown or poorly defined; even more remains to be understood about regulation their networks. A global omics analysis can provide insights into metabolism, without a priori assumptions structure these networks.With future goal predictive...
This paper examines processes in plants for the formation of fate products TNT beyond its aminated reduction products, 2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene. metabolites were isolated characterized combination with temporal analyses product profiles 14C distribution, microbe-free, axenic root cultures Catharanthus roseus. Four unique TNT-derived compounds isolated. Using evidence from 1H NMR, mass spectroscopy, HPLC, acid hydrolysis, enzymatic hydrolysis β-glucuronidase...
Metabolic flux maps developed from 13C metabolic analysis (13C MFA) are effective tools for assessing the response of biological systems to genetic or environmental perturbations, and identifying possible engineering targets. Experimental treatments were designed distinguish between temperature effects prior to, during incubation in vitro, on primary metabolism developing soybeans. Biomass accumulation increased with as did carbon partitioning into lipids. The through plastidic oxidative...
Construction of microbial biocatalysts for the production biorenewables at economically viable yields and titers is frequently hampered by product toxicity. Membrane damage often deemed as principal mechanism this toxicity, particularly in regards to decreased membrane integrity. Previous studies have attempted engineer with goal increasing However, most these works focused on engineering phospholipids efforts identify proteins that can be targeted improve fatty acid been unsuccessful.Here...
The shikimate pathway serves an essential role in many organisms. Not only are the three aromatic amino acids synthesized through this pathway, but secondary metabolites also derive from it. Decades of effort have been invested into engineering Saccharomyces cerevisiae to produce and its derivatives. In addition ability express cytochrome P450, S. is generally recognized as safe for producing compounds with nutraceutical pharmaceutical applications. However, intrinsically complicated...
TNT transformation processes in sediment-free, "natural", aquatic phytoremediation systems of Myriophyllum aquaticum were investigated with specific interest oxidation products. Extraction procedures combining liquid−liquid extractions and solid-phase developed for the isolation mostly acidic, oxidized metabolites. Six compounds unique from reduction products isolated characterized by UV−vis, 1H, 13C NMR spectroscopy, mass chemical synthesis where feasible. These include...
Abstract Different plant species produce a variety of terpenoid indole alkaloids, which are interest as defensive secondary metabolites and valuable pharmaceuticals. Although significant progress has been made, the mechanisms regulating levels this important class compounds require continued elucidation. Previous precursor feeding studies have indicated that alkaloid accumulation can be improved during exponential growth phase hairy root cultures through enhanced tryptophan availability. To...