A5004478837- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Phytoplasmas and Hemiptera pathogens
- Biochemical and Structural Characterization
- Tissue Engineering and Regenerative Medicine
- Transgenic Plants and Applications
- Cardiac electrophysiology and arrhythmias
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Sarcoma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Analytical Methods in Pharmaceuticals
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Ion channel regulation and function
- Bacillus and Francisella bacterial research
- Neuropeptides and Animal Physiology
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- melanin and skin pigmentation
- Epilepsy research and treatment
- Animal Genetics and Reproduction
- Nuclear Receptors and Signaling
- ATP Synthase and ATPases Research
Jagiellonian University
2015-2023
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2021-2022
Polish Academy of Sciences
2020-2022
The properties of mesenchymal stem cells (MSCs), especially their self-renewal and ability to differentiate into different cell lines, are widely discussed. Considering the fact that MSCs isolated from perinatal tissues reveal higher differentiation capacity than most adult MSCs, we examined Wharton's jelly umbilical cord (WJ-MSCs) in terms pluripotency markers expression. Our studies showed WJ-MSCs express some markers-such as NANOG, OCT-4, SSEA-4-but comparison iPS expression level is...
Abstract There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess safety, feasibility, potential efficacy autologous bone marrow cell transplantation in pediatric with DRE. Two females two males (11 months 6 years) were enrolled underwent combined therapy consisting nucleated cells (BMNCs) (intrathecal: 0.5 × 109; intravenous: 0.38 109–1.72 109) followed by four rounds...
Abstract Rhabdomyosarcoma (RMS) is a mesenchymal tumor of soft tissue in children that originates from myogenic differentiation defect. Expression SNAIL transcription factor elevated the alveolar subtype RMS (ARMS), characterized by low status and high aggressiveness. In patients level increases with higher stage. Moreover, negatively correlates MYF5 expression. The human ARMS cells diminishes level. silencing inhibits proliferation induces vitro , thereby completely abolishes growth...
Plants employ different chemicals to protect themselves from herbivory. These defenses may be constitutive or triggered by stress. The can toxic, act as repellents, phagosuppressants and/or phago-deterrents. two-spotted spider mite (Tetranychus urticae) is a generalist arthropod herbivorous pest and its feeding causes extensive damage both crops wild plants. Cyclotides are cyclic peptides involved in host-plant defenses. A single Viola sp. produce more than hundred cyclotides with biological...
Turnover rates have implications for understanding cyclotide biology and improving plant cell culture-based production systems. Cyclotides are a family of polypeptides recognized broad spectrum bioactivities. The cyclic, cystine knot structural motif imparts these peptides with resistance to temperature, chemicals proteolysis. found widely distributed across the Violaceae in five other families, where their presumed biological role is host defense. Violets produce mixtures different...
Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood and young adulthood. Conventional treatment consisting of surgery, chemotherapy radiotherapy can be insufficient, as long-term survival chances decrease dramatically when cancer recurrence occurs. Due to this fact, efficient still a demanding issue, thus, novel innovative therapies have considered part combined treatment. In present study, we effective suicide gene therapy rhabdomyosarcoma cell line Rh30 involving herpes...
Cyclotides are macrocycle peptides produced by plants from several families, including Violaceae. These compounds have the potential for applications in medicine, bioengineering and crop protection thanks to their multiple biological activities. In most cases, cyclotides extracted plant material. Plant cell culture provides a viable sustainable form of biomass production host defense peptides. The aim current study was test whether different stress hormones elicitors effects on cyclotide...
Targeting of the TRAIL-DR4/5 pathway was proposed as a promising approach for specific induction apoptosis in cancer cells. Clinical trials, however, showed inadequate efficiency TRAIL monotherapy. It is widely held view that application multifunctional molecules or combination therapy may lead to substantial improvement. Here, we demonstrate effectiveness and safety novel chimeric protein, AD-O51.4, which equipped with positively charged VEGFA-derived effector peptides. The study performed...
Objectives It has been reported that matrix metalloproteinase, MMP-3 may play a significant role in the pathophysiology of mental disorders. However, there are no data on level people suffering from schizophrenia, or its influence state these people. The aim this study was to investigate effect an antipsychotic treatment blood levels MMP-3, as well investigating relationship with insight into schizophrenia.Methods Thirty schizophrenia were included study. concentration serum assessed using...
Melanin is a black/brown pigment present in abundance human skin. Its main function photo-protection of underlying tissues from harmful UV light. Natural sources isolated melanin are limited; thus, vitro cultures cells may be promising source melanin. Here, we an innovative differentiation protocol induced pluripotent stem (iPS) into melanin-producing cells, delivering highly pigmented quantity and quality incomparably higher than any other methods previously described. Pigmented constitute...
Rhabdomyosarcoma (RMS), is the most frequent soft tissue tumor in children that originates from disturbances differentiation process. Mechanisms leading to development of RMS are still poorly understood. Therefore, by analysis two RH30 cell line subclones, one subclone PAX7 negative, while second positive, and comparison with other lines we aimed at identifying new mechanisms crucial for progression. subclones were characterized same STR profile, but different morphology, rate proliferation,...
Abstract Parkinson’s disease (PD) is a neurodegenerative that an increasing threat to aging society. The idiopathic form of PD accounts for over 90% all cases, and the current etiology still unknown. One reasons hindering research on this lack appropriate animal models. Among mouse models disease, those based administration neurotoxins such as 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) or 6-hydroxydopamine (6-OHDA) substantia nigra pars compacta (SNpc) striatum are predominantly...
Abstract Information coding in the hippocampus relies on interplay between various neuronal ensembles. We discovered that application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity gamma range, induces matrix metalloproteinase 9 (MMP-9)—an enzyme necessary for maintenance synaptic plasticity. Using electrophysiological recordings hippocampal organotypic slices, we show Cch potentiates frequency miniature inhibitory and excitatory postsynaptic currents (mIPSCs...
Background:The transmural differences of action potential duration (APD), across scar borderzone, are poorly defined in the intact human heart.Heterogeneity APD ventricular wall may play an important part generation and sustainment three-dimensional tachycardia (VT) circuits.Purpose: This study aimed to investigate endocardial epicardial borderzone patients admitted for VT ablation.Methods: 6 (age 63 þ/-5yrs, 5Male); 1 myocarditis, 3 DCM 2 ischemic cardiomyopathy (Mean EF 30%), who were...