A5010975119- Neuroscience and Neuropharmacology Research
- Protease and Inhibitor Mechanisms
- Memory and Neural Mechanisms
- Signaling Pathways in Disease
- S100 Proteins and Annexins
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Nuclear Receptors and Signaling
- Peptidase Inhibition and Analysis
- Stress Responses and Cortisol
- Alzheimer's disease research and treatments
- Neuroendocrine regulation and behavior
- Nerve injury and regeneration
- MicroRNA in disease regulation
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Retinoids in leukemia and cellular processes
- Amino Acid Enzymes and Metabolism
- Retinal Development and Disorders
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2016-2025
Polish Academy of Sciences
2014-2025
Stichting Technisch Centrum vd Keramische Industrie
2004-2024
Poznan University of Medical Sciences
2022
Institute of Physiology and Pathology of Hearing
2021
Institute of Photonic Sciences
2016
Lublin Oncology Center
2013
Institute of Neurobiology
2011-2013
Laboratory of Molecular Genetics
2008-2011
Medical University of Lublin
2006
Although the molecular, cellular, and systems mechanisms required for initial memory processing have been intensively investigated, those underlying permanent storage remain elusive. We present neuroanatomical, pharmacological, genetic results demonstrating that anterior cingulate cortex plays a critical role in remote contextual fear conditioning. Imaging of activity-dependent genes shows is activated by this activation impaired null α-CaMKII mutation blocks memory. Accordingly, reversible...
Matrix metalloproteinases (MMPs) are extracellular proteases that have well recognized roles in cell signaling and remodeling many tissues. In the brain, their activation function customarily associated with injury or pathology. Here, we demonstrate a novel role for MMP-9 hippocampal synaptic physiology, plasticity, memory. protein levels proteolytic activity rapidly increased by stimuli induce late-phase long-term potentiation (L-LTP) area CA1. Such regulation requires NMDA receptors...
Neurons of adult brain are able to remodel their synaptic connections in response various stimuli. Modifications the peridendritic environment, including extracellular matrix, likely play a role during synapse remodeling. Proteolytic disassembly ECM is complex process using regulated actions specific proteinases. One best-characterized families matrix-modifying enzymes matrix metalloproteinase (MMP) family. Here, we describe changes expression and function two well known MMPs, MMP-9 MMP-2,...
While a number of oncogenes are expressed in cell cycle-dependent manner, their role the control proliferation can only be established by direct functional assay. The c-myc protein, upon microinjection into nuclei quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma stimulation cellular DNA synthesis. This suggests that like platelet-derived growth factor (PDGF), may act as competence cycle to promote progression cells S phase. presence medium an antibody against PDGF abolished...
Dicer-dependent noncoding RNAs, including microRNAs (miRNAs), play an important role in a modulation of translation mRNA transcripts necessary for differentiation many cell types. In vivo experiments using type-specific Dicer1 gene inactivation neurons showed its essential neuronal development and survival. However, little is known about the consequences loss miRNAs adult, fully differentiated neurons. To address this question, we used inducible variant Cre recombinase (tamoxifen-inducible...
Temporal lobe epilepsy (TLE) is a devastating disease in which aberrant synaptic plasticity plays major role. We identify matrix metalloproteinase (MMP) 9 as novel enzyme and key pathogenic factor two animal models of TLE: kainate-evoked pentylenetetrazole (PTZ) kindling–induced epilepsy. Notably, we show that the sensitivity to PTZ epileptogenesis decreased MMP-9 knockout mice but increased line transgenic rats overexpressing MMP-9. Immunoelectron microscopy reveals associates with...
We have identified a cDNA whose sequence is preferentially expressed when quiescent fibroblasts are stimulated to proliferate. The steady-state levels of the mRNA corresponding this clone, called 2A9, increased by serum, platelet-derived growth factor, and epidermal but not insulin or platelet-poor plasma. 2A9 also in human acute myeloid leukemia. has been molecularly cloned from an Okayama-Berg library, its complete nucleotide determined. It open reading frame 270 nucleotides, which 55%...
We have established the complete coding sequence of human vimentin gene. It had 91% homology to Syrian hamster gene (Quax et al., Cell 35:215-223, 1983) and partial several other sequences for intermediate filament proteins. The most striking difference between genes was in 3' untranslated region, which considerably longer hamster. Using RNA blots a cDNA clone from an Okayama-Berg library, we that expression growth regulated. steady-state levels cytoplasmic mRNA 3T3 cells were increased by...
An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies the central nervous system but also neuronal plasticity. Here, we use three independent experimental models to show enzymatic activity MMP-9 causes elongation and thinning dendritic spines in hippocampal neurons. These are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated cultures,...
We have identified in a human cDNA library clone (hp2F1) whose cognate RNA is growth-regulated. The insert has been sequenced and the nucleotide sequence shows strong homology to sequences of ADP/ATP carrier gene, respectively, isolated from Neurospora crassa Saccharomyces cerevisiae. putative amino acid hp2F1 an 87% beef heart mitochondria. conclude that contains full coding carrier. steady-state levels are They increase when quiescent cells stimulated by serum, platelet-derived growth...
Matrix metalloproteinase-9 (MMP-9) has emerged as a physiological regulator of NMDA receptor (NMDAR)-dependent synaptic plasticity and memory. The pathways by which MMP-9 affects NMDAR signaling remain, however, elusive. Using single quantum dot tracking, we demonstrate that enzymatic activity increases NR1-NMDAR surface trafficking but no influence on AMPA mobility. mechanism action is not mediated change in overall extracellular matrix structure nor direct cleavage subunits, rather through...
Adult neurogenesis (i.e., proliferation and differentiation of neuronal precursors in the adult brain) is responsible for adding new neurons dentate gyrus hippocampus olfactory bulb. We describe herein that mice mutated cell cycle regulatory gene Ccnd2, encoding cyclin D2, lack newly born both these brain structures. In contrast, genetic ablation D1 does not affect neurogenesis. Furthermore, we show D2 only D-type (out D1, D3) expressed dividing cells derived from present hippocampus. all...
Matrix metalloproteinase-9 has recently emerged as an important molecule in control of extracellular proteolysis the synaptic plasticity. However, no targets for its enzymatic activity had been identified before. In this report, we show that β-dystroglycan comprises such a neuronal activity-driven target matrix metalloproteinase-9. This notion is based on following observations. (i) Recombinant, autoactivating produces limited proteolytic cleavage β-dystroglycan. (ii) cultures, occurs...
The memory of fear extinction is context dependent: that suppressed in one readily renews another. Understanding the underlying neuronal circuits is, therefore, considerable clinical relevance for anxiety disorders. Prefrontal cortical and hippocampal inputs to amygdala have recently been shown regulate retrieval memories, but cellular organization these projections remains unclear. By using anterograde tracing a transgenic rat which neurons express dendritically-targeted PSD-95:Venus fusion...
Abstract Fragile X syndrome (FXS) is a neurodevelopmental disorder characterized by lack of the FMR1 protein, FMRP, translational repressor. Its absence leads to up‐regulation locally translated proteins involved in synaptic transmission and plasticity, including matrix metalloproteinase‐9 (MMP‐9). In Fmr1 knock‐out (KO), mouse model FXS, an abnormal elevated expression MMP‐9 brain was pharmacologically down‐regulated after treatment with tetracycline derivative minocycline. Moreover, rescue...