A5036221013- Phosphodiesterase function and regulation
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Ion channel regulation and function
- Cholinesterase and Neurodegenerative Diseases
- Cardiac pacing and defibrillation studies
- Heart Failure Treatment and Management
- Neuropeptides and Animal Physiology
- Carcinogens and Genotoxicity Assessment
- Air Quality and Health Impacts
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Patient-Provider Communication in Healthcare
- Cardiomyopathy and Myosin Studies
- Colorectal Cancer Screening and Detection
- Calpain Protease Function and Regulation
- Chemical Safety and Risk Management
- Medical Education and Admissions
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Mycotoxins in Agriculture and Food
- Healthcare Decision-Making and Restraints
- BRCA gene mutations in cancer
- Ethics in medical practice
- COVID-19 and Mental Health
Lebanese American University
2019-2025
Université Paris Cité
2006-2012
Inserm
2005-2012
Sorbonne Université
2011-2012
Institut National de Recherche en Santé Publique
2011
Université Paris-Sud
2005-2011
Centre National de la Recherche Scientifique
2009
Deutsche Forschungsgemeinschaft
2008
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2007-2008
Laboratory Signaling and Cardiovascular Pathophysiology
2006
Compartmentation of cAMP is thought to generate the specificity Gs-coupled receptor action in cardiac myocytes, with phosphodiesterases (PDEs) playing a major role this process by preventing diffusion. We tested hypothesis adult rat ventricular myocytes characterizing PDEs involved regulation signals and L-type Ca2+ current (I(Ca,L)) on stimulation beta1-adrenergic receptors (beta1-ARs), beta2-ARs, glucagon (Glu-Rs) prostaglandin E1 (PGE1-Rs). All but PGE1-R increased total cAMP, inhibition...
Steady-state activation of cardiac beta-adrenergic receptors leads to an intracellular compartmentation cAMP resulting from localized cyclic nucleotide phosphodiesterase (PDE) activity. To evaluate the time course changes in different compartments, brief (15 seconds) pulses isoprenaline (100 nmol/L) were applied adult rat ventricular myocytes (ARVMs) while monitoring beneath membrane using engineered nucleotide-gated channels and within cytosol with fluorescence resonance energy...
β-Adrenergic receptors (β-ARs) enhance cardiac contractility by increasing cAMP levels and activating PKA. PKA increases Ca2+-induced Ca2+ release via phosphorylation of L-type channels (LTCCs) ryanodine receptor 2. Multiple cyclic nucleotide phosphodiesterases (PDEs) regulate local concentration in cardiomyocytes, with PDE4 being predominant for the control β-AR–dependent signals. Three genes encoding are expressed mouse heart: Pde4a, Pde4b, Pde4d. Here we show that both PDE4B PDE4D...
Multiple cyclic nucleotide phosphodiesterases (PDEs) degrade cAMP in cardiomyocytes but the role of PDEs controlling signaling during pathological cardiac hypertrophy is poorly defined.Evaluate beta-adrenergic regulation contractility and characterize changes cardiomyocyte signals cAMP-PDE expression activity following hypertrophy.Cardiac was induced rats by thoracic aortic banding over a time period 5 weeks confirmed anatomic measurements echocardiography. Ex vivo myocardial function...
Abstract Background Against the backdrop of Lebanon’s escalating multifaceted crisis which resulted in medication shortages for chronic diseases and unaffordable healthcare services, current study endeavors to shed light on a critical yet overlooked facet Lebanese economic crisis– its profound impact health-seeking behaviors patients with hypertension diabetes mellitus. Methods An exploratory cross-sectional based an online questionnaire was conducted 156 adult citizens diagnosed medically...
Recent studies indicate that members of the multidrug-resistance protein (MRP) family belonging to ATP binding cassette type C (ABCC) membrane proteins extrude cyclic nucleotides from various cell types. This study aimed determine whether MRP regulate cardiac cAMP homeostasis. Here, we demonstrate MRP4 is predominant isoform present at plasma cardiacmyocytes and it mediates efflux in these cells. MRP4-deficient mice displayed enhanced myocyte formation, contractility, hypertrophy 9 mo age,...
Background COVID-19 has worsened burnout, marked by exhaustion, cynicism, and reduced professional efficacy. In Lebanon, economic collapse, political instability, the Beirut Port explosion, social unrest have compounded this, with limited gender-specific data. This study evaluates burnout levels in Lebanese women men during COVID-19, exploring gender differences related factors, including mitigation strategies. Methods A cross-sectional survey included 423 adult participants aged 18 or older...
Gasoline exposure has been widely reported in the literature as being toxic to human health. However, exact underlying molecular mechanisms triggered by its inhalation have not thoroughly investigated. We herein present a model of sub-chronic, static gasoline vapor adult female C57BL/6 mice. Animals were exposed daily either vapors (0.86 g/animal/90 min) or ambient air for 5 days/week over 7 consecutive weeks. At end study period, and inflammatory, oxidative, apoptotic effects vapors,...
Abstract Diabetic cardiomyopathy (DCM) is associated with differential and time‐specific regulation of β‐adrenergic receptors cardiac cyclic nucleotide phosphodiesterases consequences for total adenosine 3′‐5′ monophosphate (cAMP) levels. We aimed to investigate whether these changes are downstream impairments in cAMP Ca 2+ signalling a type 1 diabetes (T1D)‐induced DCM model. T1D was induced adult male rats by streptozotocin (65 mg/kg) injection. assessed structural molecular remodelling....
As Medicine shifts from a paternalistic practice to patient-centered approach, the concept of medical informed consent (IC) has evolved safeguard patient autonomy. However, its current implementation still presents many challenges in clinical practice. We assessed knowledge and attitudes general Lebanese population regarding IC process as well their sociodemographic correlates. An anonymous online survey was distributed using social media channels. A sample 500 adults with an average age...
BackgroundBreast cancer (BC) has been increasing in both prevalence and incidence Lebanon. Knowing the positive impact mammographic screening on reducing mortality rates, we sought to investigate knowledge, attitudes barriers towards BC amongst Lebanese women across all districts.MethodsWe conducted a cross-sectional study with 400 aged 35–75, no prior or current diagnosis of BC, employing an online questionnaire filled face-to-face participants gather sociodemographic data assess history...
Global research on gender bias has highlighted key trends in discrimination and inequality across various fields, including medicine. In Lebanon, a nation celebrated for its diverse cultural landscape increasing female representation medical education, there been notable absence of studies exploring dynamics the profession. To address this gap, we conducted cross-sectional study using an online survey to explore Lebanese population's perceptions towards physicians quality care they provide....
Rationale: Transporters of the ABCC family, called MRP4 and MRP5, have been described to control cAMP homeostasis by extruding it from various cell types. Objective: The aim our study was determine role in heart function its impact on cardiac hypertrophy (CH). Methods results: KO mice with overexpression HCN-based sensor were used. Echocardiography morphology studies showed that 3 month-old displayed normal (FS: 35.7% ± 1.02 vs 36% 1.97 WT) phenotype (HW/TL: 7.59 0.14 WT 7.75 0.15 KO) basal...