A5027079699- Viral Infections and Immunology Research
- Cytomegalovirus and herpesvirus research
- Parasitic Infections and Diagnostics
- RNA Research and Splicing
- Plant Virus Research Studies
- Biochemical and Molecular Research
- RNA modifications and cancer
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
Umeå University
2021-2024
Abstract Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases in humans. Their rapid multiplication depends on remodeling of cytoplasmic membranes for viral genome replication. It is unknown how virions assemble around these newly synthesized genomes and they then loaded into autophagic release through secretory autophagy. Here, we use cryo-electron tomography infected cells to show poliovirus assembles directly replication membranes. Pharmacological...
Enteroviruses are abundant pathogens of humans and animals. Their replication is strictly dependent on the conserved, viral AAA+ ATPase 2C. 2C an oligomerizing, peripheral membrane protein, its low solubility as recombinant protein has hampered functional studies full-length, bound to a membrane. Here, we describe modification classical, ultracentrifugation-based liposome flotation assay optimized study interaction with membranes functions membrane-bound, full-length The takes advantage high...
The endosomal sorting complex required for transport (ESCRT) machinery constitutes multisubunit protein complexes that play an essential role in membrane remodeling and trafficking. ESCRTs regulate a wide array of cellular processes, including cytokinetic abscission, cargo into multivesicular bodies (MVBs), repair, autophagy. Given the versatile functionality ESCRTs, intricate organizational structure ESCRT machinery, targeted modulation distinct is considerably challenging. This study...
Viruses are masters at using cellular pathways to aid their replication. Cryo-electron tomography of poliovirus-infected cells revealed how it utilizes macroautophagy its advantage. Assembly these non-enveloped virions takes place directly on membranes and requires PIK3C3/VPS34 activity be completed, whereas the canonical autophagy inducer ULK1 restricts virus assembly. The tomograms further that enterovirus-induced is selective for RNA-loaded virions, which may help ensure maximum...
Enteroviruses are a vast genus of positive-sense RNA viruses that cause diseases ranging from common cold to poliomyelitis and viral myocarditis. They encode membrane-bound AAA+ ATPase, 2C, has been suggested serve several roles in virus replication, e.g. as an helicase capsid assembly factor. Here, we report the reconstitution full-length, poliovirus 2C's association with membranes. We show N-terminal membrane-binding domain 2C contains conserved glycine, which is by structure predictions...
Abstract The protozoan parasite Giardia intestinalis is one of only a few organisms lacking de novo synthesis DNA building blocks (deoxyribonucleotides). Instead, the relies exclusively on salvaging deoxyadenosine and other deoxyribonucleosides from its host environment. Here, we report that G. has deoxyribonucleoside kinase with 1000-fold higher catalytic efficiency (kcat/KM) for than corresponding mammalian kinases can thereby provide sufficient triphosphate levels despite lack synthesis....
Abstract Enteroviruses are non-enveloped positive-sense RNA viruses that cause diverse diseases in humans. Their rapid multiplication depends on remodeling of cytoplasmic membranes for viral genome replication. It is unknown how virions assemble around these newly synthesized genomes and they then loaded into autophagic release through secretory autophagy. Here, we use cryo-electron tomography infected cells to show poliovirus assembles directly replication membranes. Pharmacological...
Abstract The Endosomal Sorting Complex Required for Transport (ESCRT) machinery constitutes a multisubunit protein complex that plays an essential role in membrane remodeling and trafficking. ESCRTs regulate wide array of cellular processes, encompassing cytokinetic abscission, cargo sorting into multivesicular bodies (MVBs), repair autophagy. Given the versatile functionality intricate organizational structure ESCRT complex, targeted modulation distinct ESCRT-mediated deformations...
Abstract Enteroviruses are a vast genus of positive-sense RNA viruses that cause diseases ranging from common cold to poliomyelitis and viral myocarditis. They encode membrane-bound AAA+ ATPase, 2C, has been suggested serve several roles in virus replication, e.g. as an helicase capsid assembly factor. Here, we report the reconstitution full-length, poliovirus 2C’s association with membranes. We show N-terminal membrane-binding domain 2C contains conserved glycine, which is by structure...
Giardia intestinalis is a protozoan parasite causing giardiasis, severe, sometimes even life-threatening, diarrheal disease. one of only few known organisms that lack de novo synthesis DNA building blocks, and the therefore completely dependent on salvaging deoxyribonucleosides from host. The deoxyribonucleoside kinases (dNKs) needed for this salvage are generally divided into two structurally distinct families, thymidine kinase 1 (TK1)-like dNKs non-TK1-like dNKs. We have characterized G....