A5035615223- Insect Resistance and Genetics
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Hepatitis B Virus Studies
- Transgenic Plants and Applications
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Collagen: Extraction and Characterization
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Chromium effects and bioremediation
- Polysaccharides and Plant Cell Walls
- Sugarcane Cultivation and Processing
- Plant Gene Expression Analysis
- Nanoparticles: synthesis and applications
- Medicinal Plants and Neuroprotection
- Microbial Metabolic Engineering and Bioproduction
- Viral gastroenteritis research and epidemiology
University of Gujrat
2012-2025
Abbottabad University of Science and Technology
2023
COMSATS University Islamabad
2023
Abbott (Germany)
2023
Pakistan Agricultural Research Council
2011
Institute of Crop Science
2009-2010
Quaid-i-Azam University
2009-2010
Phenylalanine ammonia-lyase (PAL) is the first enzyme in phenylpropanoid pathway and plays a vital role adoption, growth, development plants but wheat its characterization still not very clear. Here, we report genome-wide identification of TaPAL genes analysis their transcriptional expression, duplication, phylogeny wheat. A total 37 that cluster into three subfamilies have been identified based on phylogenetic analysis. These are distributed 1A, 1B, 1D, 2A, 2B, 2D, 4A, 5B, 6A, 6B, 6D...
ABSTRACT Tomato leaf curl New Delhi virus (ToLCNDV), a begomovirus, that causes severe curling, stunting, and reduced yield in tomato plants is consistently threatening its production worldwide. CRISPR/Cas9‐mediated genome editing has shown immense potential developing disease‐resistant crops. This study successfully focuses on designing precise efficient strategy for planta defence against ToLCNDV. Five key targets within the viral genome, essential replication pathogenicity, were selected....
REVIEW article Front. Plant Sci., 15 February 2013Sec. Proteomics and Protein Structural Biology Volume 4 - 2013 | https://doi.org/10.3389/fpls.2013.00021
An indigenous strain of Trichoderma viride produced high titers cellulase complex in solid-state bio-processing agro-industrial orange peel waste, which was used as the growth-supporting substrate. When conditions SSF medium containing 15 g (50% w/w moisture) inoculated with 5 mL inoculum were optimal, maximum productions endoglucanase (655 ± 5.5 U/mL), exoglucanase (412 4.3 and β-glucosidase (515 3.7 U/mL) recorded after 4 days incubation at pH 35 °C. The enzyme activity (endoglucanase)...
Green synthesis of metallic nanoparticles has attracted a great deal attention from scientific community due to its biocompatibility and environment friendly nature. In the present study, silver were biologically synthesized using leave extracts Litchi chinensis. Biosynthesized characterized their applications observed by different methodologies. Bio-reduction reaction was confirmed surface plasmon resonance at 417 nm through UV-VIS spectrophotometer. FTIR analysis revealed that amine groups...
Agro-industrial residues are primarily composed of complex polysaccharides that strengthen the microbial growth for production industrially important enzymes like cellulases. In present study we aimed to characterize Exo 1, 4-β glucanase was indigenously produced from Trichoderma viride MBL. T. MBL cultured in Solid-State medium orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity 412 ± 12 U/mL recorded after 4th day incubation at pH 5.5 30℃. 4.17-fold...
Objective: To investigate the role of Mentha piperita silver nanoparticle-loaded carbopol gel for enhanced wound healing in a diabetic rat model. This research further aims to explore bioactive compounds derived from obtained high altitude. Method: Methanolic extracts (MP), spicata (MS) and longifolia (ML) were used synthesise nanoparticles (AgNP). AgNP synthesis was confirmed by ultraviolet–visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy...
Jasmonic acid (JA) and salicylic (SA) are signaling molecules that play key roles in the regulation of metabolic processes, reproduction, defense against pathogens. The proteomics approach was used to identify proteins induced by JA SA tomato cultivars Roma Pant Bahr, which susceptible resistant bacterial wilt, respectively. Threonine deaminase leucine amino peptidase were upregulated, ribulose-1,5-bisphosphate carboxylase/oxygenase small chain downregulated time-course application JA....
A standardized protocol was developed to enhance the in vitro regeneration efficiency of five varieties tomato from hypocotyls and leaf disc with involvement coconut water. Different concentrations IAA kinetin were used alone combination 12% Significant differences for regeneration, time taken regenerate number primordial observed different treatments, type explant, use water also among varieties. Higher obtained Avinash followed by Roma Rio Grande. Maximum 95.75% 0.5 mgL -1 IAA, 1.5 Kinetin...
Abstract Background Avian hepatitis E virus (aHEV) has been associated with hepatitis-splenomegaly syndrome (HSS) in chickens along asymptomatic subclinical infection many cases. So far, four genotypes have described, which cause chickens, specifically broiler breeders and layer chickens. In the present study, we isolated identified two novel aHEV strains from bile of Pakistan evincing clinical symptoms related to HSS. Methodology Histology liver spleen tissues was carried out observe...
Among the wheat biotic stresses, Sitobion avenae is one of main factors devastating yield per hectare. The study’s objective was to find out laccase (lac) efficacy; as a potential RNAi target against grain aphids. lac (Salac) confirmed by Reverse Transcriptase-PCR. Gene sequenced and accession number “ON703252” allotted GenBank. ERNAi tool used design 143 siRNA dsRNA target. 69% mortality 61% reduction in expression were observed 8D-post DsRNA feeding. Phylogenetic analysis displayed...
Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation cellulose, an important component plant cell wall. The present study was aimed at enhancing production endo-1, Bacillus halodurans Escherichia coli. A putative Egl gene Halodurans expressed E. coli by cloning pET 22b (+). On induction isopropyl-b-d-1-thiogalactopyranoside,...