Abstract The usage of gelatin hydrogel is limited due to its instability and poor mechanical properties, especially under physiological conditions. Divalent metal ions present in such as Ca 2+ Fe play important roles the molecule interactions. objective this study was determine impact divalent ion removal on stability properties gels with without chemical crosslinking. solution purified by Chelex resin replace sodium ions. gel then chemically crosslinked 1-ethyl-3-(3-dimethylaminopropyl)...

The application of cell-derived extracellular matrix (ECM) in tissue engineering has gained increasing interest because it can provide a naturally occurring, complex set physiologically functional signals for cell growth. ECM scaffolds produced from decellularized fibroblast sheets contain high amounts substances, such as collagen, elastin, and glycosaminoglycans. They serve adhesion sites mechanically strong supports tissue-engineered constructs. An efficient method that largely remove...

Split thickness skin graft (STSG) implantation is one of the standard therapies for full wound repair when autologous grafts (FTG) or flap transplants are inapplicable. Combined transplantation STSG with dermal substitute could enhance its therapeutic effects but results remain unsatisfactory due to insufficient blood supply at early stages, which causes necrosis and fibrosis. Human mesenchymal stem cell (hMSC) sheets capable accelerating healing process. We hypothesized that...

Native tissues are endowed with a highly organized nanofibrous extracellular matrix (ECM) that directs cellular distribution and function. The objective of this study is to create purely natural, uniform, aligned nano­fibrous ECM scaffold for potential tissue engineering applications. Synthetic nanogratings (130 nm in depth) used direct the growth human dermal fibroblasts up 8 weeks, resulting uniform 70 μm‐thick fibroblast cell sheet cells nanofibers. A natural uniformly nanofibers 78 ± 9...

Natural extracellular matrices (ECM) derived from native tissues or cultured cells are extensively employed to fabricate biocompatible scaffolds living tissue constructs for the application in cellular and engineering. The composition structure of ECM not only heterogeneous, but also cell specific. Recapitulating unique niche, ECM-based products promising quickly integrate with host accelerate restoration function. A variety natural have been biomanufactured using different approaches....

Composite nanocoating of poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) and aqueous dispersion carbon nanotubes (CNT-PSS) on lignocellulose wood microfibers has been developed to make conductive paper sheets. To construct the multilayers microfibers, cationic poly(ethyleneimine) (PEI) used in alternate deposition with anionic PEDOT-PSS solubilized CNT-PSS. Using a Keithley microprobe measurement system, current-voltage measurements have carried out single composite after...

Human mesenchymal stem cell (hMSC) sheets hold great potential in engineering three-dimensional (3D) completely biological tissues for diverse applications. Conventional sheet culturing methods employing thermoresponsive surfaces are cost ineffective, and rely heavily on available facilities. In this study, a cost-effective method of layer-by-layer grafting was utilized covalently binding homogenous collagen I layer commonly used polydimethylsiloxane (PDMS) substrate surface order to improve...

Due to the indispensable role of periosteum in bone defect healing and regeneration, a promising method enhance osteogenesis grafts by using an engineered biomimetic would be highly beneficial. The stromal microenvironment is composed various organized extracellular matrix (ECM) fibers, so aligned natural ECM sheet, derived from human dermal fibroblast cell may advantageous when applied for artificial fabrication. Human mesenchymal stem cells (hMSCs) have been used replace osteoprogenitor...

Nitric oxide (NO) plays an important role in cardiovascular homeostasis, immune responses, and wound repair. The pro-angiogenic antimicrobial properties of NO has stimulated the development NO-releasing materials for dressings. Gelatin, abundant natural biodegradable polymer derived from collagen, is able to promote S-Nitroso-N-acetylpenicillamine (SNAP) can release under physiological conditions when exposed light. objective this project was fabricate a gelatin-based nanofibrous matrix with...

There is a large demand for tissue engineered vascular grafts the application of reconstruction surgery or in vitro drug screening model. The extracellular matrix (ECM) composition along with structural and mechanical anisotropy native blood vessels critical to their functional performance. objective this study develop biomimetic graft recapitulating anisotropic features by employing nanofibrous aligned fibroblast-derived ECM human mesenchymal stem cells (hMSCs). nanotopographic cues direct...

Multicellular human mesenchymal stem cell (hMSC) spheroids have been demonstrated to be valuable in a variety of applications, including cartilage regeneration, wound healing, and neoangiogenesis. Physiological relevant low oxygen culture can significantly improve vitro hMSC expansion by preventing differentiation. We hypothesize that hypoxia-cultured better maintain the regenerative properties hMSCs. In this study, were fabricated using hanging drop method cultured under 2% O2 20% for up 96...

3D tissue based on human mesenchymal stem cell (hMSC) sheets offers many interesting opportunities for regenerating multiple types of connective tissues. Prevascularizing hMSC with endothelial cells (ECs) will improve performance by supporting survival and accelerating integration host tissue. It is hypothesized that hypoxia cultured can promote microvessel network formation preserve stemness hMSCs. This study investigates the vascularization under different oxygen tensions. found HN...

Abstract During tissue injury and wound healing process, there are dynamic reciprocal interactions among cells, extracellular matrix (ECM), mediating molecules which crucial for functional repair. Nitric oxide (NO) is one of the key that can positively regulate various biological activities involved in healing. Various ECM components serve as binding sites cells molecules, further stimulate cellular activities. Human mesenchymal stem (hMSCs) migrate to site contribute regeneration through...

Abstract: Infection-related complications have been a critical issue for the application of titanium orthopedic implants. The use Ag nanoparticles offers potential approach to incorporate antimicrobial properties into In this work, novel and simple method was developed synthesis (II) oxide deposited TiO 2 nanotubes (TiNTs) using electrochemical anodization followed by electroplating processes in same electrolyte. quantities AgO TiNT were controlled selecting different times voltages. It...

Nitric oxide plays important roles in cardiovascular homeostasis, immune responses and wound repair. Therefore, polymers that release nitric locally at the surface exhibit improved biocompatibility for biomedical implants through reducing neointimal hyperplasia thrombosis caused by blood vessel wall damage. The objective of this article was to fabricate a oxide–releasing gelatin hydrogel can continuously generate physiologically relevant level inhibit cell attachment proliferation. donor,...

Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing endothelial cells (ECs), then further used as building blocks for engineering 3D complex tissues. In addition, predifferentiation hMSCs into a tissue-specific lineage in vitro has been proven promote graft...

Event Abstract Back to Engineered mesenchymal stem cell anisotropic tissue for potential muscle regeneration Qi Xing1, Zichen Qian1, Mitchell Tahtinen1, Ai Hui Yap1, Keegan Yates1 and Feng Zhao1 1 Michigan Technological University, Biomedical Engineering, United States Introduction: Muscles are highly organized tissues with direction-dependent properties including extracellular matrix (ECM) organization, mechanical strength electrical conduction. It is important replicate the feature...

We design, model, optimize and fabricate cold electron cathodes that are specifically designed for X-ray sources. Micromachining patterning process, which enhanced edge effect, significantly improved its emission performance. High bonding strength was achieved by a preferred high temperature annealing results in the improvement of field properties. To check practical application, fabricated emitter vacuum sealed tested conventional tube.

Development of successful clinical treatments for peripheral nerve injury is limited due to the complications behind neural physiology. Human mesenchymal stem cells (hMSCs) have ability directly promote tissue repair and protect at site. Studies shown that hMSCs can be transplanted improve regeneration. Hypoxic culture condition has been proven maintain stemness later differentiation. In this study, we investigated effects low oxygen (O₂) (2% 5% O₂) pre-treatment...