A5062261815- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Spinal Cord Injury Research
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Spinal Dysraphism and Malformations
- Nerve injury and regeneration
Karolinska Institutet
2021-2025
Abstract Induced pluripotent stem cells (iPSCs) have significant potential for disease modeling and cell therapies. However, their wide-spread application has faced challenges, including batch-to-batch variabilities, notable distinctions when compared to embryonic (ESCs). Some of these disparities can from using undefined culture conditions the reprogramming procedure, however, precise mechanisms remain understudied. Here, we gene expression data over 100 iPSC ESC lines cultivated under...
<p dir="ltr">Human induced Pluripotent Stem Cells (iPSCs) have long been known for their great potential in disease and development modeling as well possible use cell transplantations. This thesis investigates the advantages limitations of human iPSCs. We investigated fundamentals neural lineage specifications during induction. In addition to development, we assessed therapeutic efficiency iPSCs derived epithelial stem (NES) doses a pre-clinical study spinal cord injury (SCI). Modeling...
<p dir="ltr">Human induced Pluripotent Stem Cells (iPSCs) have long been known for their great potential in disease and development modeling as well possible use cell transplantations. This thesis investigates the advantages limitations of human iPSCs. We investigated fundamentals neural lineage specifications during induction. In addition to development, we assessed therapeutic efficiency iPSCs derived epithelial stem (NES) doses a pre-clinical study spinal cord injury (SCI). Modeling...
Here, we present a revised protocol to derive neuroepithelial stem (NES) cells from human induced pluripotent cells. NES can be further differentiated into culture of neurons (90%) and glia (10%). We describe how maintain in differentiate them. In addition, show the potential use study role reactive oxygen species neuronal differentiation guideline for cell transfection. For complete details on execution this protocol, please refer Calvo-Garrido et al. (2019); Falk (2012).
Post-traumatic syringomyelia (PTS) affects patients with chronic spinal cord injury (SCI) and is characterized by progressive deterioration of neurological symptoms. To improve surgical treatment, we studied the therapeutic effects neuroepithelial-like stem cells (NESCs) derived from induced pluripotent (iPSCs) in a rat model PTS. facilitate clinical translation, NESCs Good Manufacturing Practice (GMP)-compliant iPSCs.Human GMP-compliant iPSCs were used to derive NESCs. Cryo-preserved...
Preclinical transplantations using human neuroepithelial stem (NES) cells in spinal cord injury models have exhibited promising results and demonstrated cell integration functional improvement transplanted animals. Previous studies relied on the generation of research grade lines continuous culture. Using fresh presents logistic hurdles for clinical transition regarding time resources maintaining high quality standards. In this study, we generated a good manufacturing practice (GMP)...
Summary Human pluripotent stem cells (hPSCs) have significant potential for disease modeling and cell therapies. However, their clinical applicability is limited due to the need undefined conditions PSC cultivation, which increase risk of immunogenicity, result in batch-to-batch variability finite scalability. These limitations may be circumvented by xeno-free, defined culture conditions. biological processes that preserve robust, homogenous PSCs remain characterized. Here, we compared gene...