A5091834710- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Spinal Cord Injury Research
- Pluripotent Stem Cells Research
- Receptor Mechanisms and Signaling
- Service and Product Innovation
- Alzheimer's disease research and treatments
- Congenital heart defects research
- Neurotransmitter Receptor Influence on Behavior
- Mesenchymal stem cell research
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Neurological disorders and treatments
- Innovative Approaches in Technology and Social Development
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Nuclear Receptors and Signaling
- Congenital gastrointestinal and neural anomalies
- Healthcare Decision-Making and Restraints
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
Karolinska Institutet
2015-2024
Karolinska University Hospital
2002-2021
Uppsala University
1988-2020
Stockholm South General Hospital
2012-2018
Integrated Cardio Metabolic Centre
1991-2018
Swedish Foundation for Strategic Research
2006-2017
Stockholm Gerontology Research Center Foundation
2013
Karlstad University
2010-2011
Palo Alto Veterans Institute for Research
2002
University of Miami
2002
Purpose The paper seeks to introduce a new perspective on the roles of customers and companies in creating value by outlining customer‐based approach service. customer's logic is examined in‐depth as being foundation customer‐dominant (CD) marketing business logic. Design/methodology/approach authors argue that both goods‐ service‐dominant are provider‐dominant. Contrasting provider‐dominant with CD logic, examines creation service from perspectives value‐in‐use, own context, experience...
The adult human brain comprises more than a thousand distinct neuronal and glial cell types, diversity that emerges during early development. To reveal the precise sequence of events development, we used single-cell RNA sequencing spatial transcriptomics uncovered states trajectories in brains at 5 to 14 postconceptional weeks (pcw). We identified 12 major classes are organized as ~600 states, which map anatomical domains pcw. described detailed differentiation forebrain midbrain found large...
Abstract The lung contains numerous specialized cell types with distinct roles in tissue function and integrity. To clarify the origins mechanisms generating heterogeneity, we created a comprehensive topographic atlas of early human development. Here report 83 states several spatially resolved developmental trajectories predict interactions within defined niches. We integrated single-cell RNA sequencing transcriptomics into web-based, open platform for interactive exploration. show gene...
<i>Background/Aims:</i> Degeneration of cholinergic neurons in the basal forebrain correlates with cognitive decline patients Alzheimer’s disease (AD). Targeted delivery exogenous nerve growth factor (NGF) has emerged as a potential AD therapy due to its regenerative effects on animal models. Here we report results first-in-man study encapsulated cell (EC) biodelivery NGF primary objective explore safety and tolerability. <i>Methods:</i> This was an open-label,...
One hallmark of Alzheimer disease (AD) is the extracellular deposition amyloid beta-peptide (Abeta) in senile plaques. Two major forms Abeta are produced, 40 (Abeta40) and 42 (Abeta42) residues long. The most abundant form Abeta40, while Abeta42 more hydrophobic prone to toxic oligomers species particular importance early plaque formation. Thus, length C-terminal seems be very important for oligomerization neurotoxicity peptide. Here we investigated which deposited AD brain. We analyzed...
Abstract Background Targeted delivery of nerve growth factor (NGF) has emerged as a potential therapy for Alzheimer’s disease (AD) due to its regenerative effects on basal forebrain cholinergic neurons. This hypothesis been tested in patients with AD using encapsulated cell biodelivery NGF (NGF-ECB) first-in-human study. We report our results from third-dose cohort receiving second-generation NGF-ECB implants improved secretion. Methods Four mild moderate were recruited participate an...
Oligodendrogenesis in the human central nervous system has been observed mainly at second trimester of gestation, a much later developmental stage compared to oligodendrogenesis mice. Here, we characterize transcriptomic neural diversity forebrain post-conception weeks (PCW) 8-10. Using single-cell RNA sequencing, find evidence emergence first wave oligodendrocyte lineage cells as early PCW 8, which also confirm epigenomic level through use ATAC-seq. regulatory network inference, predict key...
Abstract The human brain develops through a tightly organized cascade of patterning events, induced by transcription factor expression and changes in chromatin accessibility. Although gene across the developing has been described at single-cell resolution 1 , similar atlases accessibility have primarily focused on forebrain 2–4 . Here we describe paired entire during first trimester (6–13 weeks after conception). We defined 135 clusters used multiomic measurements to link candidate cis...
Diffuse hemispheric gliomas, H3G34R/V-mutant (DHG-H3G34), are lethal brain tumors lacking targeted therapies. They originate from interneuronal precursors; however, leveraging this origin for therapeutic insights remains unexplored. Here, we delineate a cellular hierarchy along the interneuron lineage development continuum, revealing that DHG-H3G34 mirror spatial patterns of progenitor streams surrounding nests, as seen during human development. Integrating these findings with genome-wide...
Background & AimsThe enteric nervous system (ENS) regulates gastrointestinal function via different subtypes of neurons, organized into fine-tuned neural circuits. It is not clear how cell diversity created within the embryonic ENS; information required for development cell-based therapies and models neuropathies. We aimed to identify proteins that regulate ENS differentiation network formation.MethodsWe generated compared RNA expression profiles entire ENS, progenitor cells, non-ENS gut...
Changes in cell identities and positions underlie tissue development disease progression. Although single-cell mRNA sequencing (scRNA-Seq) methods rapidly generate extensive lists of states, spatially resolved mapping presents a challenging task. We developed SCRINSHOT (Single-Cell Resolution IN Situ Hybridization On Tissues), sensitive, multiplex RNA approach. Direct hybridization padlock probes on is followed by circularization with SplintR ligase rolling circle amplification (RCA) the...
Abstract The spatiotemporal regulation of cell fate specification in the human developing spinal cord remains largely unknown. In this study, by performing integrated analysis single-cell and spatial multi-omics data, we used 16 prenatal samples to create a comprehensive developmental atlas during post-conceptional weeks 5–12. This revealed how commitment neural progenitor cells their positioning are spatiotemporally regulated specific gene sets. We identified unique events development...