A5025074763- Zebrafish Biomedical Research Applications
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Congenital gastrointestinal and neural anomalies
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Developmental Biology and Gene Regulation
- Gastrointestinal motility and disorders
- Neural dynamics and brain function
- RNA Research and Splicing
- Neuroscience and Neuropharmacology Research
- Neuroblastoma Research and Treatments
- Digestive system and related health
- Psychosomatic Disorders and Their Treatments
- Mental Health and Psychiatry
- Bioinformatics and Genomic Networks
- Silk-based biomaterials and applications
- IL-33, ST2, and ILC Pathways
- Molecular Biology Techniques and Applications
- Olfactory and Sensory Function Studies
- Neurofibromatosis and Schwannoma Cases
- Intracranial Aneurysms: Treatment and Complications
- Adrenal and Paraganglionic Tumors
- Mental Health Research Topics
Karolinska Institutet
2014-2024
Uppsala University
2009-2022
The mammalian nervous system executes complex behaviors controlled by specialized, precisely positioned, and interacting cell types. Here, we used RNA sequencing of half a million single cells to create detailed census types in the mouse system. We mapped spatially derived hierarchical, data-driven taxonomy. Neurons were most diverse grouped developmental anatomical units expression neurotransmitters neuropeptides. Neuronal diversity was driven genes encoding identity, synaptic connectivity,...
Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left-right alternation limbs as well coordinated activation flexor and extensor muscles. Here we show premature stop codon the DMRT3 gene has major effect pattern locomotion horses. The mutation is permissive for ability to perform alternate gaits favourable harness racing performance. Examination wild-type...
Following the yellow brick road The adrenal glands affect a variety of processes such as stress responses and metabolism. mature gland is formed from multiple tissue sources, including cells neural origin. Furlan et al. traced origins these cells. first become Schwann cell precursors follow along nerves to travel dorsal root ganglia spine gland. Once there, differentiate into chromaffin authors used singlecell transcriptomics reveal shifts in functional programs during migration,...
The peripheral autonomic nervous system reaches far throughout the body and includes neurons of diverse functions, such as sympathetic parasympathetic. We show that parasympathetic in mice--including trunk ganglia cranial ciliary, pterygopalatine, lingual, submandibular, otic ganglia--arise from glial cells nerves, not neural crest cells. fate is induced nerve-associated Schwann cell precursors at distal sites. used multicolor Cre-reporter lineage tracing to most these arise bi-potent...
In humans, neurosecretory chromaffin cells control a number of important bodily functions, including those related to stress response. Chromaffin appear as distinct cell type at the beginning midgestation and are main cellular source adrenalin noradrenalin released into blood stream. mammals, two different organs emerge close distance each other, adrenal gland Zuckerkandl organ (ZO). These structures found in proximity kidneys dorsal aorta, region where paraganglioma, pheochromocytoma...
Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems affected spinal cord injury diseases such as amyotrophic lateral sclerosis (ALS) muscular atrophy. Using two screening approaches situ hybridization, we identified 159 genes expressed typical patterns cord. These include general neuron markers, one gene exclusively neurons, nine unknown subtypes of neurons. Further, present evidence that chondrolectin (Chodl) is by fast...
Background & AimsThe enteric nervous system (ENS) regulates gastrointestinal function via different subtypes of neurons, organized into fine-tuned neural circuits. It is not clear how cell diversity created within the embryonic ENS; information required for development cell-based therapies and models neuropathies. We aimed to identify proteins that regulate ENS differentiation network formation.MethodsWe generated compared RNA expression profiles entire ENS, progenitor cells, non-ENS gut...
Neuronal circuits in the spinal cord that produce rhythmic and coordinated activities necessary for limb movements are referred to as locomotor central pattern generators (CPGs). The identities preceding development of neurons essential coordination between left right limbs not yet known. We show ventral floor plate chemoattractant Netrin-1 preferentially guides dorsally originating subtypes commissural interneurons, majority which inhibitory. In contrast, excitatory ventralmost V3 subtype...
The enteric nervous system (ENS) is organized into neural circuits within the gastrointestinal wall where it controls peristaltic movements, secretion, and blood flow. Although proper gut function relies on complex neuronal composition of ENS, little known about transcriptional networks that regulate diversification different classes neurons glia during development. Here we redefine role Ascl1 (Mash1), one few regulatory transcription factors described ENS We show all subtypes appear to be...
INTRODUCTION: White matter lacks the plasticity present in cortex, and subcortical injuries such as traumatic brain spinal cord injury, often result permanent neurological deficits. Because cortical regions share common nuclei, creating new intergyral connections may allow for bypass of damage. In this manuscript, a surgical interhemispheric bridge is created mice, providing model an intercortical transpial bypass. METHODS: A midline craniotomy followed by (IH) pial removal was performed...
Schizophrenia is an often devastating disorder characterized by persistent and idiopathic cognitive deficits, delusions hallucinations. has been associated with impaired nervous system development excitation/inhibition imbalance in the prefrontal cortex. On a molecular level, schizophrenia moderately heritable genetically complex. Hundreds of risk genes have identified, spanning heterogeneous landscape dominated loci that confer relatively small risk. Bioinformatic analyses genetic...
Abstract The mammalian nervous system executes complex behaviors controlled by specialised, precisely positioned and interacting cell types. Here, we used RNA sequencing of half a million single cells to create detailed census types in the mouse system. We mapped spatially derived hierarchical, data-driven taxonomy. Neurons were most diverse, grouped developmental anatomical units, expression neurotransmitters neuropeptides. Neuronal diversity was driven genes encoding identity, synaptic...
Cortical parvalbumin-expressing (Pvalb + ) neurons provide robust inhibition to neighboring pyramidal neurons, crucial for the proper functioning of cortical networks. This class inhibitory undergoes extensive synaptic formation and maturation during first weeks after birth continue dynamically maintain their output throughout adulthood. While several transcription factors, such as Nkx2-1, Lhx6, Sox6, are known be necessary differentiation progenitors into Pvalb which transcriptional...
Current textbooks teach that adrenergic chromaffin cells of the adrenal medulla originate from a sympathoadrenal cell lineage neural crest nearby dorsal aorta. Here, Furlan et al. demonstrate novel origin these neuroendocrine arising predominantly Schwamm precursors (SCP) peripheral nerves. Previously it had been shown SCPs serve as multipotent stem can differentiate into various types, most importantly parasympathetic nerve cells. Using genetic fate tracing, SCP and ablation, well...
ABSTRACT Autonomous functions of the gastrointestinal tract require combined activity functionally distinct neurons enteric nervous system (ENS). However, range neuron diversity and how it emerges during development remain largely unknown. We here make a novel molecular definition 12 classes (ENCs) within myenteric plexus mouse small intestine. identify communication features provide histochemical markers for discrete motor, sensory, interneurons together with genetic tools class-specific...
Abstract White matter lacks the kind of plasticity that is present in cortex, and subcortical injuries often result permanent neurological deficits. Because cortical regions share common nuclei, creating new intergyral connections may allow for bypass damage. In this manuscript, a surgical interhemispheric bridge created mice, providing model an intercortical transpial bypass. To bypass, midline craniotomy followed by (IH) pial removal was performed C57BL/6 allowing juxtaposition right left...
<h3>Abstract</h3> Identifying the spinal circuits controlling locomotion is critical for unravelling mechanisms production of gaits. Development governing left-right coordination relies on axon guidance molecules such as ephrins and netrins. To date, no other class proteins have been shown to play a role during this process. Here, we analyzed <i>hop</i> mice, which walk with characteristic hopping gait using their hindlimbs in synchrony. Fictive experiments suggest that local defect ventral...