A5050919617- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Hybrid Renewable Energy Systems
- Cancer-related gene regulation
Australian National University
2021-2024
Sapienza University of Rome
2020-2021
N6-methyladenosine (m6A) is an RNA modification well-known for its contribution to different processes controlling metabolism, including splicing, stability, and translation of mRNA. Conversely, the role m6A on biogenesis function circular RNAs (circRNAs) has yet be addressed. circRNAs belong a class covalently closed transcripts produced via back-splicing reaction whereby downstream 5′ splice donor site fuses upstream 3′ acceptor site. Starting from circ-ZNF609 as study case, we discover...
Abstract The epitranscriptome embodies many new and largely unexplored functions of RNA. A significant roadblock hindering progress in epitranscriptomics is the identification more than one modification individual transcript molecules. We address this with CHEUI (CH3 (methylation) Estimation Using Ionic current). predicts N6-methyladenosine (m6A) 5-methylcytosine (m5C) molecules from same sample, stoichiometry at reference sites, differential methylation between any two conditions. processes...
RNA modifications are important regulators of transcript activity and an increasingly emerging body data suggests that the epitranscriptome its associated enzymes altered in human tumors.
ABSTRACT The epitranscriptome embodies many new and largely unexplored functions of RNA. A significant roadblock hindering progress in epitranscriptomics is the identification more than one modification individual transcript molecules. We address this with CHEUI (CH3 (methylation) Estimation Using Ionic current). predicts N6-methyladenosine (m6A) 5-methylcytidine (m5C) molecules from same sample, stoichiometry at reference sites, differential methylation between any two conditions. processes...
Abstract mRNA synthesis requires extensive pre-mRNA maturation, the organisation of which remains unclear. Here, we directly sequence without metabolic labelling or amplification to resolve transcription and multimodal processing at single-molecule resolution. Using poly(A) tail measurement, distinguish transcriptionally engaged from polyadenylated transcripts, revealing that splicing is substantially delayed compared previous estimates. Splicing rare for least 10 kb behind elongating RNA...
The potential presence of 5-methylcytosine as a sparse internal modification mRNA was first raised in 1975, and map the also part epitranscriptomics “big bang” 2012. Since then, evidence for its has firmed up, initial insights have been gained into molecular function broader biological relevance when present mRNA. Here, we summarize status quo field, outline some current challenges, suggest how to address them future work.
Maps of the RNA modification 5-methylcytosine (m 5 C) often diverge markedly not only because differences in detection methods, data depth and analysis pipelines but also biological factors. We re-analysed bisulfite sequencing datasets from five human cell lines seven tissues using a coherent m C site calling pipeline. With resulting union list 6,393 sites, we studied distribution, enzymology, interaction with RNA-binding proteins molecular function. confirmed tRNA:m methyltransferases NSUN2...
Circular RNAs (circRNAs), a class of covalently closed formed by back-splicing reaction, have been involved in the regulation diverse oncogenic processes. In this article we describe circVAMP3, novel circular RNA overexpressed RH4, representative cell line alveolar rhabdomyosarcoma. We demonstrated that circVAMP3 has differential m6A pattern opposed to its linear counterpart, suggesting two isoforms can be differently regulated such modification. Moreover, show how depletion rhabdomyosarcoma...