Abstract Viral diseases cause significant harm to human health and often high mortality. In the past twenty years, humanity has undergone infection by SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East syndrome) COVID-19 coronaviruses, which spread from animals humans person person. These have led large economic losses. To fight coronaviruses other viruses, it is proposed use miRNAs, regulate protein synthesis at translational level. MirTarget program was used determine...

Background/Objectives: microRNAs are small non-coding RNAs that regulate gene expression by inducing mRNA degradation or inhibiting translation. A growing body of evidence suggests miRNAs may be utilized as anti-cancer therapeutics targeting key genes involved in cancerous transformation and progression. Renal cell cancer (RCC) is the most common kidney malignancy. The efficient RCC treatments involve blockers immune checkpoints, including antibodies PD-L1 (Programmed Death Ligand 1)....

A prolonged pandemic with numerous human casualties requires a rapid search for means to control the various strains of SARS-CoV-2. Since only part population is affected by coronaviruses, there are probably endogenous compounds preventing spread these viral pathogens. It has been shown that piRNA (PIWI-interacting RNAs) interact mRNA genes and can block protein synthesis at stage translation. Estimated effects on SARS-CoV-2 genomic RNA (gRNA) in silico. cluster 13 binding sites (BS) gRNA...

Exogenous microRNAs (miRNAs) enter the human body through food, and their effects on metabolic processes can be considerable. It is important to determine which miRNAs from plants affect expression of genes extent influence.

Multiple sclerosis (MS) is a common inflammatory demyelinating disease with high mortality rate. MS caused by many candidate genes whose specific involvement has yet to be established. The aim of our study was identify endogenous miRNAs and piRNAs involved in the regulation gene expression using bioinformatic methods. A program used quantify interaction miRNA piRNA nucleotides mRNA target genes. We 7310 from three databases 40,000 piRNAs. mRNAs revealed binding sites (BSs), which were...

Abstract The possibility of using miRNA (mRNA-inhibiting RNA) to inhibit infections caused by the coronaviruses COVID-19, SARS-CoV, and MERS-CoV has been shown. Using bioinformatics approaches, completely complementary (cc-miRNA) complexes were predicted be able bind translation coronavirus proteins replication genomes. For seven cc-miRc for cc-miRs eight cc-miRm MERS-CoV, interactions with RNA genomes (gRNAs) corresponding was evaluated. free energy cc-miRNAs binding sites significantly...

Abstract Background: In the past twenty years humankind has effected from infection caused by SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East syndrome) and COVID-19 coronaviruses, which have significant harm to human health resulted in high mortality. The possibility of using miRNA (mRNA-inhibiting RNA) inhibit infections coronaviruses COVID-19, SARS-CoV, been shown. Methods: MirTarget program determines following characteristics interaction between miRNAs messenger RNAs...

Abstract Background: In the past twenty years humankind has effected from infections caused by SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East syndrome) and SARS-CoV-2 coronaviruses, which have significant harm to human health resulted in high mortality. The possibility of using miRNA (mRNA-inhibiting RNA) inhibit coronaviruses SARS-CoV-2, SARS-CoV, been shown. Methods: MirTarget program determines following characteristics interaction between miRNAs messenger RNAs...

Modern methods of increasing plant productivity are based on knowledge the molecular basis growth and development. The key regulators these processes transcription factors (TF) that control expression genome at all stages ontogenesis. significant part TF genes depends miRNAs, therefore, it is necessary to find out which miRNAs what extent they can affect TFs. In present work, using MirTarget program, we identified capable affecting GRAS (gibberellic-acid insensitive, repressor gai...

Background: Human consumption of food and herbal medicines supplies the body with not only nutrients but also associated biologically active compounds, such as exogenic messenger RNA (mRNA) inhibitory RNAs (miRNAs). Therefore, it seems important to establish which maize wheat miRNAs can enter human evaluate their possible actions binding features mRNA genes. It is required determine whether most common plant interact genes what consequences this interaction may have. Methods: The...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that caused the COVID-19 pandemic still able to infect population in many countries. The Omicron strain is most mutated variant of SARS-CoV-2. high transmissibility and ability evade immunity require a priority study its properties order quickly create effective means preventing it. present work devoted silico interaction piRNAs with genome SARS-CoV-2 (gRNA) identify endogenous propose synthetic antiviral activity for drug...

Recently, a database of human piRNAs has been created, which allows studying the binding many to mRNAs genes involved in diseases, including cancer. In present work, we investigated what can interact with candidate esophageal squamous cell carcinoma (ESCC) genes. The 480 thousand 66 ESCC was studied. Bioinformatic studies found that bind only nine genes: AURKA, BMP7, GCOM1, ERCC1, MTHFR, SASH1, SIX4, SULT1A1, and TP53. It shown mRNA by overlapping nucleotide sequences limited 3'UTR 5'UTR...

Plant miRNAs are able to enter the human cells through gastrointestinal tract with food and affect processes occurring in body like endogenous miRNAs.These exogenous can regulate expression of genes, affecting various physiological processes.The interactions mRNAs were calculated using MirTarget program.Rice chosen, because rice, comparison other plants, contains largest amount is most common nutrition population.It was found that 32 genes targets for 17 osa-miRNAs (miR408-3p, miR408-5p,...

miRNA regulates the expression of a large number genes, including plant transcription factors. It is important to reveal associations with corresponding targets in order increase productivity and resistance biotic abiotic stresses. One such group MYB factor involved regulation some most physiological processes. The search for target genes was performed using MirTarget program, which calculates free energy (ΔG) binding mRNA; position schemes potential sites. relative amount (ΔG/ΔGm) used as...

Abstract Exogenous miRNAs enter the human body through food, and their effects on metabolic processes can be considerable. It is important to determine which from plants affect expression of genes extent influence. The binding sites 738 osa-miRNAs that interact with 17508 mRNAs were determined using MirTarget program. characteristics 46 single 86 a value free energy (ΔG) interaction equal 94% 100% maximum ΔG established. findings showed osa-miR2102-5p, osa-miR5075-3p, osa-miR2097-5p,...

Data on the involvement of miRNA in plant response to stress factors are considered.The MYB family transcription plays a great role development, metabolism and responses biotic abiotic stress.The free energy binding, value interaction, position schemes potential binding sites were calculated using MirTarget program.To identify miRNAs whose targets genes family, search for 738 mRNAs 124 Oryza sativa was performed.16 identified as 11 miRNAs.The results show that can regulate expression most...

The molecular mechanisms for increasing plant productivity remain poorly understood. Genes of C2H2, GRAS, ERF transcription factors (TFs) families play a key role in the physiological processes plants, including wheat. In recent years, important miRNAs regulation expression many genes involved formation has been established. Wheat miRNA (mRNA-inhibiting RNA) target are development flowers, seeds, root, shoots, and responses to abiotic biotic stresses. binding sites mRNAs ERF, GRAS TFs were...

The molecular mechanisms for increasing plant productivity remain poorly understood.Genes of C2H2, GRAS, ERF transcription factors (TFs) families play a key role in the physiological processes plants, including wheat.In recent years, important miRNAs regulation expression many genes involved formation has been established.Wheat miRNA (mRNA-inhibiting RNA) target are development flowers, seeds, root, shoots, and responses to abiotic biotic stresses.The binding sites mRNAs ERF, GRAS TFs were...