A5102998286- Rheumatoid Arthritis Research and Therapies
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Spinal Fractures and Fixation Techniques
- Immune Response and Inflammation
- Orthopaedic implants and arthroplasty
- Hematopoietic Stem Cell Transplantation
- Total Knee Arthroplasty Outcomes
- Orthopedic Infections and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Renal Diseases and Glomerulopathies
- Osteoarthritis Treatment and Mechanisms
- Hip disorders and treatments
- Respiratory viral infections research
- CCD and CMOS Imaging Sensors
- Cytomegalovirus and herpesvirus research
- Renal Transplantation Outcomes and Treatments
- Pharmaceutical studies and practices
- Acute Lymphoblastic Leukemia research
- Molecular Communication and Nanonetworks
- Virus-based gene therapy research
- Neurosurgical Procedures and Complications
- Caching and Content Delivery
Tokyo Metropolitan University
2024
Hokkaido University Hospital
2023
Sapporo City General Hospital
2020
Tokyo University of Agriculture and Technology
2018-2019
Meitetsu Hospital
2018
NTT (Japan)
2012-2016
Mitsubishi Electric (United States)
2015
Saitama Medical University
2009
Nagoya City University
2001-2007
Nishi Niigata Chuo National Hospital
2006
A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from helper T-cell library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express products encoded full-length inserts. By assaying transfected cell supernatants, we identified clones encoding factor stimulates mast lines. This also induces Ia on resting B enhances IgG1 IgE...
A cDNA sequence coding for a human interleukin has been isolated from concanavalin A-activated T-cell library based on homology with mouse that expresses B-cell stimulatory factor 1 (BSF-1) activity and T-cell- mast-cell-stimulating activities. The contains single open reading frame encoding protein of 153 amino acid residues including putative signal peptide. Amino sequences the polypeptides, deduced their cDNAs, share extensive exception about 40 near middle portion, which little homology....
A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from libraries prepared mRNA derived concanavalin A-activated T-cell clones. The constructed in the pcD vector system were screened by transfecting COS-7 monkey cells with DNA pools to express products encoded full-length inserts. By assaying cell supernatants, we identified clones encoding that stimulates formation of granulocyte and macrophage colonies progenitor cells. These results...
Objective. Systemic lupus erythematosus (SLE) in a multisystem autoimmune disorder characterized by multiorgan pathology and autoantibodies against variety of autoantigens. The CD28 CTLA‐4 genes might be candidate for SLE, because costimulation signals from CD80/CD86 to CD28/CTLA‐4 have been suggested play an important role the activation or inactivation T lymphocytes. Methods. We investigated three polymorphic regions within gene, C/T base exchange promoter region −318 (CTLA‐4 −318C/T), A/G...
Objectives. The possible link between LEF and interstitial lung disease (ILD) has evoked increasing concern. aim of the present study was to elucidate prevalence risk factors for newly developed and/or exacerbated ILD, based on post-marketing surveillance data, in which all RA patients receiving were pre-registered monitored 24 weeks Japan. Methods. We analysed data from a cohort 5054 who prescribed since its launch September 2003 Multivariable logistic analysis performed identify...
We have isolated cDNA clones encoding human IL-3 from libraries constructed in a modified pcD mammalian expression vector by using mRNA prepared activated T cell clones. Amino acid sequence of deduced DNA these agrees with that predicted genomic except at amino position 27. Northern blotting analysis and S1 nuclease show almost all express kinetics similar to observed mouse However, striking difference was found the level granulocyte-macrophage-CSF expressed cells. In contrast clones, is...
The complete nucleotide sequences of the 5.8S and 26S rRNA genes Physarum polycephalum transcribed spacer between them were determined. Comparison with those Escherichia coli 23S yeast showed that there are 16 highly homologous regions in E. eukaryotes have some eukaryote-specific extra sequences. sequence immediately following 5.8S-like region was found to be 5' terminus rRNA, indicating gene is derived from 5'-terminal prokaryote large gene.
Monocyte chemoattractant protein-1 (MCP-1) is up-regulated and recruits activates inflammatory cells in human diffuse proliferative lupus nephritis (DPLN) of model MRL/lpr mice. The aim this study was to examine whether anti-MCP-1 gene therapy inhibits the progression mice.An NH(2)-terminal deletion mutant MCP-1 gene, 7ND, injected into skeletal muscles mice with advanced stage blockade its receptor (CCR2) signalling pathway.Histological findings kidneys treated mice, which received more...
Since IL-10 has recently been shown to exhibit pleiotropic effects on human monocytes, it was of interest determine the effect this cytokine prostaglandin E2 (PGE2) production by monocytes. Recombinant (rIL-10) did not significantly affect PGE2 lipopolysaccharide (LPS)-unstimulated but efficiently inhibited LPS-stimulated The inhibition rIL-10 achieved in a dose-dependent manner. IL-4 also at same degree as rIL-10. Viral monocytes similar fashion Endogenously produced inhibit Kinetic studies...
Objective. To elucidate the factors associated with poor prognosis of LEF-induced lung injury in patients RA. Methods. The background and clinical laboratory features were examined compared between who died recovered from it. Results. Among 22 developed injury, 9 13 tended to have pre-existing interstitial pneumonia (8/9 vs 6/13, P = 0.07). loading maintenance doses, serum concentration LEF metabolite A771726 administration period did not differ groups. Patients had more frequently...
Using the urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration, effects of participation in a two-day ultramarathon race period on oxidative DNA damage were investigated Japanese nonprofessional runners. Before first day (baseline), after (mid-race) 40-km running, and second (post-race) 90 km biomaterials successfully sampled from 95 participants (males, 79; females, 16) who completed full race. We analyzed urine for 8-OHdG blood aspartate aminotransferase (AST), creatine phosphokinase...
Donor-specific antibodies (DSAs) are the main cause of graft loss over time. The direct pathway alloantigen recognition is important in pathogenesis acute rejection. Recent studies have suggested that also contributes to chronic injury. Nevertheless, there no reports on T-cell response via kidney recipients with DSAs. We analyzed DSAs (DSA+) or without (DSA−). A mixed lymphocyte reaction assay was implemented assess response. DSA+ patients showed significantly higher CD8 + and CD4 T cell...
Human immune response can be classified into 2 different subsets of T helper cells (Th1 and Th2) based on the pattern cytokine production. In modern immunology, Th1/Th2 paradigm helps to explain inflammatory effector pathways outcomes in human diseases. The present study was designed determine type immunological that influences anti-neutrophil cytoplasmic antibody-(ANCA) associated glomerulonephritis (GN) using analysis peripheral diseased kidney tissues.We analyzed blood ratio 91 patients...
[Purpose] This work was designed to establish criteria for assessing common clinical measurement methods thoracic spinal rotation angles by comparing their results with magnetic resonance imaging measurements. [Participants and Methods] Twenty-six healthy participants underwent angle assessments using an electronic goniometer in three positions: lumbar-locked, seated, half-kneeling. We compared these measurements obtained imaging. [Results] A moderate but significant positive correlation...
Abstract We introduce a microrubbing process using small hot stylus to create liquid crystalline (LC) polymer possessing microscale thread-like structure with good molecular orientation properties. In this process, the spin-coated thin film was rubbed apply lateral shear force and induce LC phase. The effects of line thickness (or created thread spacing) on processing temperature vertical load were studied construct formation model threads. Furthermore, regular arrangement threads, which can...