The present study evaluated the effects of dietary onion (Allium cepa)powder and its extracts on growth, blood biochemical parameters, non-specific immune parameters potential disease resistance against pathogenic Aeromonas hydrophila infection in Oreochromis niloticus. Fish formulated diets containing powder (1% or 0.5%) alcoholic 0.5%). At end experiment, weight gain specific growth rate were significantly increased than control. Besides, red cells (RBCs) haemoglobin (Hb) showed a...

Superoxide dismutase is an important antioxidative stress enzyme which found in honeybee venom and has a wide pharmaceutical medical applications.We reported the purification characterization of SOD from Egyptian Apis mellifera lamarckii termed BVSOD. It was purified to homogeneity venom. The procedures included crude extraction, DEAE-cellulose anion exchange column chromatography, Sephacryl S-300 gel filtration chromatography. BVSOD be homogeneous as investigated by native PAGE. exhibited...

Honey bee venom contains various enzymes with wide medical and pharmaceutical applications. The phospholipase A2 (PLA2) has been apparently purified from the of Egyptian honey (Apis mellifera lamarckii) 8.9-fold to a very high specific activity 6033 U/mg protein using DEAE–cellulose Sephacryl S-300 columns. PLA2 is monomeric 16 kDa isoelectric point (pI) 5.9. optimal was attained at pH 8 45 °C. Cu2+, Ni2+, Fe2+, Ca2+, Co2+ exhibited complete activating effect on it, while Zn2+, Mn2+, NaN3,...

The study aimed to evaluate the effect of dietary inclusions Moringa oleifera on growth, haematology, antioxidant activities, immunity, and protection against Aeromonas hydrophila in Oreochromis niloticus. Diets supplemented with 0.5% or 1% either crude moringa (CM) powder alcoholic extracts (AME) were used. Weight gain (WG), specific growth rate (SGR), haematological parameters (RBCs count, Hb content HCT %) significantly improved (p < 0.05) feeding CM than control fish, meanwhile they...

Purification and characterization of xanthine oxidase from liver the water buffalo Bubalus bubalisMahmoud A. Ibrahim, Hassan M.M. Masoud, Doaa Darwish, Sayed S. Esa, Samir A.M. Zaahkouk

Bee venom is one of theeconomic income sources for beekeepers due to their widely medical uses andother traded products. This study investigates the biological activity ofvenom extracts from honeybee, Apis melliferasubspecies: A. m. lamarckii and carnica by manual electro-stimulated extracted methods. Venoms compared with regard SDS-PAGE analysis, enzymaticactivities: protease, PLA2, hyaluronidase haemolyticactivity melittin, LD50 value. Theelectrophoretic analysis data showed that venoms...

drainage channels where aquaculture projects are not allowed to use irrigation or Nile water (Soliman, 2017).Drainage was reported as contaminated with agrochemicals, industrial effluents, and domestic sewage (Khallaf

Moniezia expansa worms are a significant source of alkaline phosphatase (ALP) and acetylcholinesterase (AChE) enzymes. The current study presents simple reproducible ALP AChE purification method from helminthes by precipitating the proteins with ammonium sulfate chromatography on Sephacryl S-300 column. M. purified at 1070.8 U/mg, displaying 6.0 folds 53.6% yield, while is 5250 2.0 43% yield. isoenzyme displayed its optimum activity pH 9.6, 8.0. affinity for several substrates revealed that...

Palm tocotrienol-rich fraction supplementation suppressed stress-induced gastric oxidative stress in ratsKamisah Y., Ibrahim A.A.I., Nafeeza M.I., Nur-Azlina M.F.

Abstract The hyaluronidase enzyme has been used in many such fields of medicine as ophthalmology, orthopaedia, internal medicine, gynecology, surgery, oncology and dermatology. In this study, the was purified characterized for first time from Egyptian bee venom homogeneously using DEAE-cellulose Sephacryl S-300 columns. Bee specific activity 411.7 units/mg protein with 49.9% yield 3.23-fold purification. molecular weight native form 37 kDa. found homogeneous on PAGE SDS-PAGE, two congruent...

Abstract Background High doses of diclofenac (DF) might induce acute kidney injury (AKI), particularly in elderly, a known vulnerable population. Melatonin was found to afford beneficial effect various diseases. Aim To investigate the possible nephroprotective melatonin aged rats subjected AKI by DF, and highlight implicated mechanisms. Design Experimental study Methods Thirty male Wister rats, 22-24 month, were equally divided into: control group, DF treated group (DF-group),...

Acid phosphatase is one of the most important enzymes in venom honeybees that has many medical and pharmaceutical applications. In this study, an acid was isolated homogeneously purified from Apis mellifera lamarckii named BVACP. The purification process carried out using column chromatography on CM-cellulose Sephacryl S-300 resins. BVACP specific activity 305.4 Umg-1 obtained with 33.2% recovery 3.9 times realized. found monomer protein 46 kDa by gel electrophoresis filtration...

Abstract Background and Aim of Work Doxorubicin (DOX) is one the most commonly used effective anticancer drugs, through its inhibition topoisomerase II, DNA replication repair. In addition, DOX leads to generation semiquinone free radicals oxygen which attack oxidize bases. However, clinical use doxorubicin limited by adverse effects such as cardiotoxicity, acute occurs within 2-3 days administration. Recently, potential health benefits Astaxanthin were investigated, however, protective...

Purification and characterization of xanthine oxidase from liver the water buffalo Bubalus bubalisMahmoud A. Ibrahim, Hassan M.M. Masoud, Doaa Darwish, Sayed S. Esa, Samir A.M. Zaahkouk

Uricase enzyme is a major component of the diagnostic kit for estimation uric acid diagnosis, monitoring and treatment gout joint inflammations. Uric Acid/Uricase assay simple measuring concentrations in biological samples such as serum, plasma, urine without any need pretreatment. The level uricase activity was detected crude extract some animal liver tissues, plant leaves microbial source (Bacillus subtilis SP6 bacteria) expressed specific (unit/mg protein). It found difficult to produce...

Acid phosphatases (ACPs) perform a fundamental role in the digestion of egg proteins (vitellins), ensuring survival ticks by providing nutrients necessary for their oogenesis. Here, acid phosphatase activity is detected during embryogenesis camel tick Hyalomma dromedarii and reached its highest level larval stage. The larvae which was named TLACP homogeneously purified using CM cellulose cation exchange chromatography followed gel filtration on Sephacryl S-300 column. to 11 times...

Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation chromatography on DEAE-cellulose Sephacryl S-300 columns. xanthine homogeneously 31.8 folds 3.5 U/mg specific activity 24.1% recovery. SLXO native molecular weight 150 kDa SDS-PAGE appeared as single major band 75 representing homodimer protein....

Lactate dehydrogenase (LDH) enzyme is a major component of aspartate aminotransferase (AST) and alanine (ALT) diagnosis kits.In this work, the LDH was purified characterized from buffalo liver for direct application in preparation AST ALT kits.One (BLLDH) isoform two other secondary peaks were analyzed by diethylaminoethyl (DEAE) cellulose chromatography.BLLDH obtained ammonium sulfate sedimentation chromatographically separated on ion exchange size-exclusion matrices.The isolated BLLDH has...

Malate dehydrogenase enzyme is a major component in aspartate aminotransferase (AST) diagnostic kit that used diagnosis and monitoring of liver diseases.This study aimed at purification characterization MDH from sheep for direct application preparation AST kit.Two malate isoenzymes were resolved by chromatography on DEAE-cellulose column, one (SLMDH) another minor one.SLMDH was extracted purified ammonium sulfate fractionation chromatographical separation anion exchanger gel filtration...