Honey bee venom contains various enzymes with wide medical and pharmaceutical applications. The phospholipase A2 (PLA2) has been apparently purified from the of Egyptian honey (Apis mellifera lamarckii) 8.9-fold to a very high specific activity 6033 U/mg protein using DEAE–cellulose Sephacryl S-300 columns. PLA2 is monomeric 16 kDa isoelectric point (pI) 5.9. optimal was attained at pH 8 45 °C. Cu2+, Ni2+, Fe2+, Ca2+, Co2+ exhibited complete activating effect on it, while Zn2+, Mn2+, NaN3,...

Purification and characterization of xanthine oxidase from liver the water buffalo Bubalus bubalisMahmoud A. Ibrahim, Hassan M.M. Masoud, Doaa Darwish, Sayed S. Esa, Samir A.M. Zaahkouk

Abstract Background Faba bean attacked by soil-borne pathogens causing root rot disease. This disease has serious damage to both plant stand and produced yield. The present study aimed evaluate effectiveness of the bioagents; Trichoderma harzianum some resistance inducers as fungicide alternatives against incidence at pre- post-emergence growth stages. Results Under open greenhouse conditions , faba in phases was considerably reduced using six inorganic salts five antioxidants individually...

Moniezia expansa worms are a significant source of alkaline phosphatase (ALP) and acetylcholinesterase (AChE) enzymes. The current study presents simple reproducible ALP AChE purification method from helminthes by precipitating the proteins with ammonium sulfate chromatography on Sephacryl S-300 column. M. purified at 1070.8 U/mg, displaying 6.0 folds 53.6% yield, while is 5250 2.0 43% yield. isoenzyme displayed its optimum activity pH 9.6, 8.0. affinity for several substrates revealed that...

Palm tocotrienol-rich fraction supplementation suppressed stress-induced gastric oxidative stress in ratsKamisah Y., Ibrahim A.A.I., Nafeeza M.I., Nur-Azlina M.F.

Catalase plays a major role in protecting cells against toxic reactive oxygen species. Here, was purified from larvae of the camel tick Hyalomma dromedarii and designated TLCAT. It by ammonium sulfate precipitation chromatography on DEAE-cellulose, Sephacryl S-300 CM-cellulose columns. Gel filtration SDS-PAGE TLCAT indicated that protein has native molecular weight 120 kDa is most likely homodimer with subunit approximately 60 kDa. The Km value 12 mM H2O2 displayed its optimum activity at pH...

Thrombin is the most important enzyme in hemostatic process by permitting rapid and localized coagulation case of tissue damage. Camel thrombin natural proper target for previously purified camel tick salivary gland inhibitor.In this study, was homogenously a single affinity chromatographic step on heparin-agarose column with specific activity 3242 NIH units/mg proteins. On SDS-PAGE, contained two forms, 37 kDa α-thrombin 28 β-thrombin, prothrombin visualized as 72 kDa. The Km value found...

Eight sweet pepper plant samples showing viral and like symptoms were collected from open field used for detecting infections through biological, serological biochemical methods. DAS-ELISA, DBIA TPIA have relative effectiveness parenchymal viruses (CMV, TMV PVY) vascular virus (TYLCV), the DAS-ELISA are found more efficient (87.5%) than (78.1%). The examined leaf co-infected with different mixed types of including TMV, PVY TYLCV), (TMV, TYLCV) (TMV that enhanced degrees severe external...

Abstract The hyaluronidase enzyme has been used in many such fields of medicine as ophthalmology, orthopaedia, internal medicine, gynecology, surgery, oncology and dermatology. In this study, the was purified characterized for first time from Egyptian bee venom homogeneously using DEAE-cellulose Sephacryl S-300 columns. Bee specific activity 411.7 units/mg protein with 49.9% yield 3.23-fold purification. molecular weight native form 37 kDa. found homogeneous on PAGE SDS-PAGE, two congruent...

Purification and characterization of xanthine oxidase from liver the water buffalo Bubalus bubalisMahmoud A. Ibrahim, Hassan M.M. Masoud, Doaa Darwish, Sayed S. Esa, Samir A.M. Zaahkouk

Uricase enzyme is a major component of the diagnostic kit for estimation uric acid diagnosis, monitoring and treatment gout joint inflammations. Uric Acid/Uricase assay simple measuring concentrations in biological samples such as serum, plasma, urine without any need pretreatment. The level uricase activity was detected crude extract some animal liver tissues, plant leaves microbial source (Bacillus subtilis SP6 bacteria) expressed specific (unit/mg protein). It found difficult to produce...

Acid phosphatases (ACPs) perform a fundamental role in the digestion of egg proteins (vitellins), ensuring survival ticks by providing nutrients necessary for their oogenesis. Here, acid phosphatase activity is detected during embryogenesis camel tick Hyalomma dromedarii and reached its highest level larval stage. The larvae which was named TLACP homogeneously purified using CM cellulose cation exchange chromatography followed gel filtration on Sephacryl S-300 column. to 11 times...

A variety of soil- and air-borne pathogens can attack faba bean plants, leading to rust chocolate spot diseases that seriously affect plant yield. This study aims evaluate the efficacy certain essential oils as abiotic agents biotic safe alternatives fungicides for both diseases. Double foliar applications these significantly reduced development incidence. The highest reduction in (92.7%) was achieved after spraying with jojoba oil, followed by 88.9% 85.3% grape bitter orange oils,...

Acid phosphatase is one of the most important enzymes in venom honeybees that has many medical and pharmaceutical applications. In this study, an acid was isolated homogeneously purified from Apis mellifera lamarckii named BVACP. The purification process carried out using column chromatography on CM-cellulose Sephacryl S-300 resins. BVACP specific activity 305.4 Umg-1 obtained with 33.2% recovery 3.9 times realized. found monomer protein 46 kDa by gel electrophoresis filtration...

Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation chromatography on DEAE-cellulose Sephacryl S-300 columns. xanthine homogeneously 31.8 folds 3.5 U/mg specific activity 24.1% recovery. SLXO native molecular weight 150 kDa SDS-PAGE appeared as single major band 75 representing homodimer protein....

Lactate dehydrogenase (LDH) enzyme is a major component of aspartate aminotransferase (AST) and alanine (ALT) diagnosis kits.In this work, the LDH was purified characterized from buffalo liver for direct application in preparation AST ALT kits.One (BLLDH) isoform two other secondary peaks were analyzed by diethylaminoethyl (DEAE) cellulose chromatography.BLLDH obtained ammonium sulfate sedimentation chromatographically separated on ion exchange size-exclusion matrices.The isolated BLLDH has...

Malate dehydrogenase enzyme is a major component in aspartate aminotransferase (AST) diagnostic kit that used diagnosis and monitoring of liver diseases.This study aimed at purification characterization MDH from sheep for direct application preparation AST kit.Two malate isoenzymes were resolved by chromatography on DEAE-cellulose column, one (SLMDH) another minor one.SLMDH was extracted purified ammonium sulfate fractionation chromatographical separation anion exchanger gel filtration...

Glutathione s-transferases (GSTs) perform an essential role in detoxification of xenobiotics and endogenous compounds via their conjugation to reduce glutathione.