A5081732951- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Liver Disease Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Endoplasmic Reticulum Stress and Disease
- Diet, Metabolism, and Disease
- Autism Spectrum Disorder Research
- Molecular Biology Techniques and Applications
- COVID-19 diagnosis using AI
- Genetics and Neurodevelopmental Disorders
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Heat shock proteins research
- Drug Transport and Resistance Mechanisms
- Advanced biosensing and bioanalysis techniques
- Regulation of Appetite and Obesity
- Animal Genetics and Reproduction
- Genetics, Bioinformatics, and Biomedical Research
- Veterinary Equine Medical Research
- Osteoarthritis Treatment and Mechanisms
- Liver physiology and pathology
- Protein Degradation and Inhibitors
- Amino Acid Enzymes and Metabolism
Shionogi (Japan)
2015-2024
Futaba (Japan)
2015-2024
Centre for Drug Research and Development
2016
Kyoto University
2012-2014
Kyoto Sangyo University
2012-2014
Chronic liver injury, often caused by alcoholism and viral hepatitis, causes fibrosis via the induction of collagen production. In fibrosis, hepatic stellate cells (HSCs) are activated transform into myofibroblasts, which actively produce secrete extracellular matrix. Hsp47 (heat shock protein 47) is a collagen-specific molecular chaperone that essential for maturation secretion collagen. Here, we used Cre-LoxP system to disrupt gene in isolated HSCs from floxed mice. Immature type I...
Heat shock protein 47 kDa (Hsp47) is considered as a molecular chaperone essential for the correct folding of type I and IV procollagen in ER. However, function Hsp47 other types its importance chondrogenesis have never been elucidated. To examine cartilage formation endochondral ossification, we conditionally inactivated gene chondrocytes using floxed mice carrying chondrocyte-specific Col2a1–Cre transgene. conditional null mutant died just before or shortly after birth, exhibited severe...
During the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several severe acute respiratory syndrome 2 (SARS-CoV-2) detection methods from wastewater have been developed, there are obstacles to their social implementation. In this study, we developed COPMAN (Coagulation Proteolysis method using Magnetic beads for...
Wastewater-based epidemiology (WBE) is a promising tool to efficiently monitor COVID-19 prevalence in community. For WBE community surveillance, automation of the viral RNA detection process ideal. In present study, we achieved near full-automation previously established method, COPMAN (COagulation and Proteolysis method using MAgnetic beads for Nucleic acids wastewater), which was then applied detect SARS-CoV-2 wastewater half year. The line employed Maholo LabDroid an automated-pipetting...
Wastewater surveillance can offer a comprehensive grasp of infectious disease prevalence and human health because wastewater contains various human-derived microbial pathogens, including viruses, bacteria, fungi. However, methods capable simultaneous detection multiple groups targets in the automated systems large-scale are still under development. Here, we demonstrated modification, involving addition bead-beating, to existing COPMAN (COagulation Proteolysis method using MAgnetic beads for...
To identify the molecular profiles of islets from alloxan (ALX)- and streptozotocin (STZ)-treated rats, a microarray-based global gene expression analysis was performed on frozen isolated via laser capture microdissection. At 6 weeks old, rats were injected with ALX (40 mg/kg) or STZ (50 100 then euthanized 24 hr later. Histopathological showed β-cell necrosis, macrophage infiltration, islet atrophy. The extent these changes more notable in groups than group. Transcriptome demonstrated...
Autism Spectrum Disorder (ASD) is a developmental condition characterized by core symptoms including social difficulties, repetitive behaviors, and sensory abnormalities. Aberrant morphology of dendritic spines within the cortex has been documented in genetic disorders associated with ASD ASD-like traits. We hypothesized that compounds ameliorate abnormalities spine dynamics might have potential to ASD. Because influenced signal inputs from other neurons various molecular interactions,...
Transchromosomic technology using mouse artificial chromosomes (MACs) offers a promising approach for transferring gene clusters into host organisms. This study focused on the multispecific organic anion-transporting polypeptides (OATPs) in liver, which exhibit significant species differences between mice (Oatp1a1/Slco1a1, Oatp1a4/Slco1a4, Oatp1b2/Slco1b2) and humans (OATP1B1/SLCO1B1 OATP1B3/SLCO1B3). We generated an OATP1-humanized transchromosomic model MAC vector (hOATP1-MAC mice) by...
During the COVID-19 pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several SARS-CoV-2 detection methods from wastewater have been developed, there are obstacles to their social implementation. In this study, we developed COPMAN (Coagulation Proteolysis method using Magnetic beads for Nucleic acids in wastewater), automatable can concentrate detect...
Wastewater surveillance can offer a comprehensive grasp of infectious disease prevalence and human health because wastewater contains variety human-derived microbial pathogens, including viruses, bacteria, fungi. In the present study, we demonstrated that modified version COPMAN (Coagulation Proteolysis method using Magnetic beads for detection Nucleic acids in wastewater), an automatable nucleic method, effectively extract detect both DNA RNA from various microorganisms wastewater. We to...