Abstract Background Exosomes are extracellular vesicles of nano-structures and represent an emerging nano-scale acellular therapy in recent years. Tendon regeneration is a sophisticated process the field microsurgery due to its poor natural healing ability. To date, no successful long-term solution has been provided for tendon injuries. Functional recovery requires advanced treatment strategies. Human umbilical cord mesenchymal stem cell-derived exosomes (HUMSC-Exos) considered as promising...

Background: Adipose stem cell–derived exosomes (ASC-Exos) are reported to effectively prevent muscle atrophy and degeneration of torn rat rotator cuff, but their influence on human samples potential mechanism still unclear. Purpose: We aimed investigate the effects ASC-Exos metabolic activities cuff tendons explore behind it. Study Design: Controlled laboratory study. Methods: Diseased supraspinatus were harvested from 15 patients with a mean ± SD age 65.8 3.2 years who underwent reverse...

Heterotopic ossification (HO) is one of the most intractable disorders following musculoskeletal injury and characterized by ectopic presence bone tissue in soft leading to severe loss function extremities. Recent studies have indicated that immune cell infiltration inflammation are involved aberrant formation. In this study, we found increased monocyte/macrophage mast accumulation during early HO progression. Macrophage depletion clodronate liposomes stabilization cromolyn sodium...

Abstract Heterotopic ossification (HO) represents an unwanted ossific wound healing response to the soft tissue injury which caused catastrophic limb dysfunction. Recent studies established involvement of inflammation and cellular senescence in process, though their role HO still remained be clarified. Here, a novel crosstalk where pyroptotic macrophages aroused tendon‐derived stem cells (TDSCs) is revealed encourage osteogenic during trauma‐induced formation. Macrophage pyroptosis blockade...

Abstract Tendon injury is a tricky and prevalent motor system disease, leading to compromised daily activity disability. Insufficient regenerative capability dysregulation of immune microenvironment are the causes functional loss. First, this work identifies persistent oxidative stress mitochondrial impairment in regional tendon tissues postinjury. Therefore, smart scaffold incorporating enzyme mimicry nanoparticle‐ceria nanozyme (CeNPs) into nanofiber bundle (NBS@CeO) with porous,...

Abstract Tendon injury is a common motor system disease, impairing joint mobility, and lowering quality of life. Once damaged, tendon has limited capacity for regeneration. Clinically, available therapeutic strategies have not achieved satisfactory outcomes. Chiral biomaterials can effectively regulate cell behaviors tissue healing, but been applied to injured yet. Here, chiral arginine attached electrospun membrane fabricate scaffolds. L‐chiral scaffold, rather than D‐chiral or R‐chiral...

As an omnipresent occurrence in the natural world, chirality plays a crucial role numerous biological and physiological processes. Therefore, incorporation of into biointerface materials has been emerging as research hotspot development regenerative biomaterials. Nevertheless, how chiral interact with organisms remains poorly understood. In current study, bioinspired self-assembled nanohydroxyapatite material is developed for bone regeneration using distraction osteogenesis model. We found...

Wear particle-induced periprosthetic inflammatory osteolysis and resultant aseptic loosening are major causes of orthopedic implant failure, for which there no effective treatments other than revision surgery. Crocin, a carotenoid compound derived from crocus flowers, has anti-inflammatory properties, but its immunomodulatory function role in not well characterized. Here we report the effect crocin on titanium (Ti) macrophage polarization osteogenic differentiation. We found that induced...

As a common complication of tendon injury, adhesion is an unresolved problem in clinical work. The aim this study was to investigate whether human umbilical cord mesenchymal stem cell-derived exosomes (HUMSC-Exos), one the most promising new-generation cell-free therapeutic agents, can improve and explore potential-related mechanisms.The rat Achilles injury model constructed vivo, localization HUMSC-Exos used evaluate adhesion. Rat fibroblast cell lines were treated with transforming growth...

Aims This study aimed to determine the minimal detectable change (MDC), clinically important difference (MCID), and substantial clinical benefit (SCB) under distribution- anchor-based methods for Mayo Elbow Performance Index (MEPI) range of movement (ROM) after open elbow arthrolysis (OEA). We also assessed proportion patients who achieved MCID SCB; identified factors associated with achieving MCID. Methods A cohort 265 treated by OEA were included. The MEPI ROM evaluated at baseline...

Abstract Background Peritendinous fibrosis represents a fibrotic healing process that usually occurs after tendon injury or surgery. This worldwide challenge hampers the functional rehabilitation and mobility of extremities. However, effective treatment is still lacking at present. The aim our study was to explore effect extracellular vesicles derived from hydroxycamptothecin primed human umbilical cord stem cells (HCPT-EVs) on post-traumatic adhesion. Methods Extracellular unprimed...

Oxidative stress has close relationship with the progression of osteoarthritis and thus is an important therapeutic target. The use antioxidants alone common disadvantages such as low bioavailability, poor stability, rapid joint clearance or toxicity at high concentrations. To achieve both reactive oxygen species (ROS) responsive scavenging ability drug targeted delivery ability, a novel polymer (PEG-PTK-PEG) was synthesized through simple direct reaction between polythioketal (PTK)...

Abstract Heterotopic ossification occurs as a pathological condition characterized by ectopic bone formation within soft tissues following trauma. Vascularization has long been established to fuel skeletal during tissue development and regeneration. However, the feasibility of vascularization target heterotopic prevention remained be further clarified. Here, we aimed identify whether verteporfin widely used FDA‐approved anti‐vascularization drug could effectively inhibit trauma‐induced...

The elbow is more susceptible to loss of motion after trauma than any other joint. Open arthrolysis often performed for posttraumatic stiffness if the does not improve with nonsurgical treatment, but midterm results this procedure and factors that may limit recovery have been widely studied.We reviewed patients who had undergone open hinged external fixator severe (ROM ≤ 60°) a minimum 5 years followup (1) analyze ROM gains; (2) assess functional improvement Mayo Elbow Performance Index...

PURPOSE: To investigate the effect of curcumin on visfatin and zinc-α2-glycoprotein (ZAG) expression levels in rats with non-alcoholic fatty liver disease (NAFLD). METHODS: Fifty-six male were randomly divided into a control group (n=16) model (n=40) fed normal diet or high-fat diet, respectively. Equal volumes sodium carboxymethyl cellulose (CMC) intragastrically administered to for 4 weeks. At end 12th week, ZAG protein examined by immunohistochemistry. Visfatin mRNA measured...

Post-traumatic elbow stiffness is a disabling condition that remains challenging for upper limb surgeons. Open arthrolysis commonly used the treatment of stiff when conservative therapy has failed. Multiple questions arise from surgeons who deal with this disease. These include whether patient post-traumatic elbow, how to evaluate problem, surgery appropriate, perform an excellent arthrolysis, what optimal postoperative rehabilitation is, and prevent or reduce incidence complications....

The prevalence of non-alcoholic fatty liver disease (NAFLD) has been increasing in recent years. Previous studies have suggested that micro (mi)RNAs may be involved the pathogenesis NAFLD. To investigate role miRNAs rat NAFLD, a total 16 male Sprague Dawley rats were randomly divided into control group and model group. Rats fed normal diet for 12 weeks, whereas high‑fat high‑sugar weeks. Following this, animals sacrificed tissues rapidly removed to severity Blood samples collected function,...