A5002901463- Neonatal and fetal brain pathology
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Neonatal Respiratory Health Research
- Cultural Insights and Digital Impacts
- Neuroendocrine Tumor Research Advances
- Regulation of Appetite and Obesity
- Neuroscience of respiration and sleep
- Traumatic Brain Injury and Neurovascular Disturbances
- French Urban and Social Studies
- Preterm Birth and Chorioamnionitis
- Aging, Elder Care, and Social Issues
- Neurobiology and Insect Physiology Research
- Anesthesia and Neurotoxicity Research
- Axon Guidance and Neuronal Signaling
- Estrogen and related hormone effects
- Pregnancy and preeclampsia studies
- Hormonal and reproductive studies
- Cooperative Communication and Network Coding
- Biochemical Analysis and Sensing Techniques
- Stress Responses and Cortisol
- Health, Medicine and Society
- Infant Development and Preterm Care
- Physiological and biochemical adaptations
Université de Rouen Normandie
2007-2023
Inserm
2008-2023
Normandie Université
2016-2021
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2017-2020
Centre Hospitalier Universitaire de Rouen
2020
Centre National de la Recherche Scientifique
1991-2017
Centre Hospitalier Universitaire Amiens-Picardie
2017
Neurosciences Paris-Seine
2017
Sorbonne Université
2017
Institute for Research and Innovation in Biomedicine
2010-2016
Periventricular leukomalacia (PVL) is the main cause of neurologic handicap in pre‐term infants. The understanding cellular and molecular mechanisms leading to white matter damage critical for development innovative therapeutic strategies PVL. pathogenesis PVL remains unclear but possibly involves glutamate excitotoxicity as an important pathway. We previously described a neonatal mouse model excitotoxic lesion mimicking human In present study, we used this experimental tool investigate...
Somatostatin and somatostatin receptors are transiently expressed in the immature rat cerebellar cortex but virtually undetectable cerebellum of adults. Although binding sites have been visualized during postnatal period external granule cell layer, type that expresses has never identified; thus, potential function developing remains unknown. In present study, we taken advantage possibility obtaining a culture preparation is greatly enriched cells to investigate presence effect on...
The role of dopaminergic and adrenergic innervation the intermediate lobe amphibian pituitary in release αMSH has been studied vitro. Neurointermediate lobes frog (Rana ridibunda Pallas) have perifused culture medium (ACM) for 5–7 h. released effluent perifusate was measured by means a sensitive specific RIA. No significant morphological alteration neurointermediate cells observed during perifusion experiment, even at electron microscopic level. existence receptors, responsible an inhibition...
The biochemical characterization and anatomical distribution of somatostatin binding sites were examined in the brain frog Rana ridibunda, receptors was compared with location immunoreactive neurons. pharmacological profile determined by means an iodinated superagonist somatostatin, [125I-Tyr0,DTrp8]S-14. Membrane-enriched preparations from homogenates shown to contain high-affinity (KD = 0.78 +/- 0.34 nM; Bmax 103 + 12.7 fmoles/mg protein) specificity for [DTrp] substituted S14 S28 analogs....
Experiments were performed to characterize vasoactive intestinal peptide (VIP) receptors in rat lung and study their localization by means of light electron microscope autoradiography. [125I]Iodo-VIP binding homogenate was inhibited synthetic VIP, [Val5]secretin, secretin, 7-27 secretin with half-maximal inhibition (ID50) values 1.62, 13, 121 nmol/liter up 0.1 mmol/liter, respectively. The relative potency [Val5]secretin displace the tracer indicated that [125I]iodo-VIP binds specific VIP...
In order to identify the anterior pituitary cell type(s) containing corticotropin-releasing factor (CRF) receptors and study internalization processes of this peptide by target cells, radioautography was performed on rat pituitaries removed at specific intervals (2-60 min) after intracarotid injection [125I]iodo-CRF into intact adrenalectomized female rats. animals, all corticotrophs were labeled, whereas in animals about 80% hypertrophied (adrenalectomy cells) were. control injected with...
Cerebral palsy remains a public health priority. Recognition of factors susceptibility to perinatal brain lesions is key for the prevention cerebral palsy. In most cases, pathophysiology these thought involve prior exposure predisposing that make developing more vulnerable events. The present study tested hypothesis chronic minimal stress throughout gestation would sensitize offspring neonatal excitotoxic lesions, which mimic observed in Pregnant mice were exposed chronic, ultramild stress,...
Ketamine, a non-competitive N-methyl-D-aspartate (NMDA) antagonist, widely used as an anesthetic in neonatal pediatrics, is also illicit drug named Super K or KitKat consumed by teens and young adults. In the immature brain, despite several studies indicating that NMDA antagonists are neuroprotective against excitotoxic injuries, there more evidence these molecules exert deleterious effect suppressing trophic function of glutamate. present study, we show using Gad67-GFP mice prenatal...
Disorders of neuronal migration in cerebral cortex are associated with neurological impairments, including mental retardation and epilepsy. Their causes pathophysiology remain largely unknown, however. In patients Zellweger disease, a lethal panperoxisomal disorder, mice lacking the Pxr1 import receptor for peroxisomal matrix proteins, absence peroxisomes leads to abnormal migration. Analysis Pxr1-/- revealed that defect was caused by altered N-methyl-D-aspartate (NMDA) glutamate...
To identify the anterior pituitary cell type(s) containing somatostatin-28 (SS-28)-binding sites and to study internalization processes of this peptide by target cells, autoradiography was performed on rat pituitaries removed at specific intervals (2–60 min) after iv injection [125I]iodo-SS-28 agonist [Leu8,d-Trp22,Tyr25]SS-28 into intact, adrenalectomized, or castrated male rats. At light microcopic level, silver grains were found in 75% cells. Concomitant an excess unlabeled prevented...
The radiolabelled somatostatin analogs [125I-Tyr°, Z)Trp8]S14 and [Leu8, DTrp22, l25I-Tyr25]S28 were used as radioligands to study the distribution of receptors in rat hypothalamus. Previous studies have detected very few somatostatin-binding sites hypothalamus using vitro autoradiography. Since lack autoradiographic labelling has been ascribed occupancy by endogenous ligands, we developed a method guanosine triphosphate (GTP) pretreatment unmask receptors. Preincubation brain slices with...