A5049746620- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Cancer Mechanisms and Therapy
- NF-κB Signaling Pathways
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Sulfur Compounds in Biology
- Galectins and Cancer Biology
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Pancreatic and Hepatic Oncology Research
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Bioinformatics and Genomic Networks
- Molecular Sensors and Ion Detection
- Lipid metabolism and disorders
- Caveolin-1 and cellular processes
- Pancreatitis Pathology and Treatment
- Polyamine Metabolism and Applications
Sun Yat-sen University
2012-2024
Eighth Affiliated Hospital of Sun Yat-sen University
2018-2024
Dana-Farber Cancer Institute
2019-2024
Harvard University
2019-2024
Putian University
2020-2024
The Eighth Hospital of Xi'an
2023
Huangshan University
2023
The Seventh Affiliated Hospital of Sun Yat-sen University
2021
Broad Institute
2019-2021
Massachusetts Institute of Technology
2021
Tumor metastasis involves a series of biological steps during which the tumor cells acquire ability to invade surrounding tissues and survive outside original site. During early stages, cancer undergo an epithelial-mesenchymal transition (EMT). Wnt/β-catenin signaling is known drive EMT metastasis. Here we report that hyperactivated in metastatic breast express microRNA 374a (miR-374a). In cell lines, ectopic overexpression miR-374a promoted both vitro vivo. Furthermore, directly targeted...
Abstract AKT signaling is constitutively activated in various cancers, due large part to loss-of-function the PTEN and PHLPP phosphatases that act as tumor suppressor genes. However, widely non–small cell lung cancers (NSCLC) where genetic alterations or genes are rare, suggesting an undefined mechanism(s) for their suppression. In this study, we report upregulation of oncomir microRNA (miR)-205 multiple subtypes NSCLC, which directly represses PHLPP2 expression activates both AKT/FOXO3a...
Abstract Cancer chemoresistance and metastasis are tightly associated features. However, whether they share common molecular mechanisms thus can be targeted with one strategy remain unclear in non-small cell lung cancer (NSCLC). Here, we report that high levels of microRNA-128-3p (miR-128-3p) is key to concomitant development residual NSCLC cells having survived repeated chemotherapy correlates chemoresistance, aggressiveness poor prognosis patients. Mechanistically, miR-128-3p induces...
Constitutive activation of NF-κB is a frequent event in human cancers, playing important roles cancer development and progression. In nontransformed cells, tightly controlled by IκBs. IκBs bind the cytoplasm, preventing it from translocating to nucleus modulate gene expression. Stimuli that activate signaling trigger IκB degradation, enabling nuclear translocation NF-κB. Among genes regulated are those encoding IκBs, providing negative feedback loop limits activity. How transformed cells...
Abstract Deeper mechanistic understanding of lung adenocarcinoma (non–small cell carcinoma, or NSCLC), a leading cause cancer-related deaths overall, may lead to more effective therapeutic strategies. In analyzing NSCLC clinical specimens and lines, we discovered uniform decrease in miR-186 (MIR186) expression comparison with normal tissue epithelial lines. correlated patient survival, median overall survival time 63.0 21.5 months cases exhibiting high low levels miR-186, respectively....
Abstract Cancer stem cells (CSCs) are involved in tumorigenesis, tumour recurrence and therapy resistance Wnt signalling is essential for the development of biological traits CSCs. In non-small cell lung carcinoma (NSCLC), unlike colon cancer, mutations β-catenin APC genes uncommon; thus, mechanism underlying constitutive activation NSCLC remains unclear. Here we report that miR-582-3p expression correlates with overall- recurrence-free-survival patients, has an activating effect on...
MicroRNAs have the capacity to coordinately repress multiple target genes and interfere with biological functions of cell, such as proliferation apoptosis. Here we report that miR‐136 is downregulated in human glioma, miRNA promotes apoptosis glioma cells induced by chemotherapy. Two anti‐apoptotic genes, AEG‐1 Bcl‐2, are identified targets miR‐136, restoration or Bcl‐2 expression suppresses miR‐136‐enhanced Therefore, might play a tumor‐suppressive role thereby represent potential...
Metastasis-associated in colon cancer-1 (MACC1) was first identified as a transcriptional activator for proto-oncogene c-MET expression, and its overexpression is frequently associated with metastatic progression multiply tumor types. In the present study, we analyzed time expression of MACC1 breast cancer correlation clinicopathologic features, including metastasis patient survival.MACC1 protein two cohorts clinicopathologically characterized using immunohistochemistry. Statistical analysis...
Abstract The RNF43 _p.G659fs mutation occurs frequently in colorectal cancer, but its function remains poorly understood and there are no specific therapies directed against this alteration. In study, we find that promotes cell growth independent of Wnt signaling. We perform a drug repurposing library screen discover cells with _p.G659 mutations selectively killed by inhibition PI3K PI3K/mTOR inhibitors yield promising antitumor activity 659mut isogenic lines xenograft models, as well...
The molecular mechanism underlying constitutive activation of AKT signaling, which plays essential roles in astrocytoma progression, is not fully characterized. Increasing numbers studies have reported that microRNAs are involved the malignant behavior cells via directly targeting multiple oncogenes or tumor suppressors. Here, we found microRNA (miR)-542-3p expression was decreased glioblastoma cell lines and tissues, reduced levels miR-542-3p correlated with high histopathological grades...
Non-small cell lung cancer (NSCLC) remains a major cause of death worldwide. As metastatic disease is primarily responsible for the poor clinical outcome NSCLC, it important to understand process, and its underlying molecular mechanism as well, via which NSCLC cells disseminate. In this study, we identified new competing endogenous RNA (ceRNA), namely, MYEOV transcript, found that upregulated in associated with prognosis disease. We further uncovered ceRNA plays critical role invasion...
Abstract Chemotherapy resistance in colorectal cancer (CRC) remains a major obstacle clinical oncology. Analysis of specimens from chemotherapy-resistant patients revealed elevated CXCL7 expression tumor-associated macrophages (TAMs). Through integrated vitro and vivo studies, we demonstrated that chemotherapy induces tumor cell-macrophage crosstalk, leading to upregulation TAMs. Using co-culture system, observed CXCL7+ confer chemoresistance CRC cells. Mechanistic investigations activates...
MicroRNA-873 (miR‑873) has been reported to be dysregulated in a variety of malignancies, however, the biological function and underlying molecular mechanism miR‑873 colorectal cancer (CRC) remain unclear. In present study we found that expression levels were markedly decreased CRC cell lines tissues from patients. Statistical analysis revealed was inversely correlated with disease stage CRC. Kaplan‑Meier survival patients lower had shorter overall rates. Additionally, downregulation...
Abstract Background Triple negative breast cancer (TNBC) is an aggressive subtype of with high proliferative activity. TNBC tumors exhibit elevated MYC expression and altered regulatory genes, which are associated tumor progression poor prognosis; however, the underlying mechanisms by retains its mediates tumorigenesis require further exploration. Methods ACTL6A regulation target gene, CDK2, was defined using Co-IP, mass spectrometry ChIP assays. To study role in TNBC, we performed...
Invasion and metastasis are main traits of tumor progression responsible for the poor prognosis advanced non-small cell lung cancer (NSCLC). The molecular mechanisms underlying malignant behaviors NSCLC remain incompletely understood. present study demonstrate that up-regulator proliferation (URGCP), a recently identified tumor-promoting gene found in several types, is markedly overexpressed human lines clinical samples. URGCP upregulation correlates significantly with this disease. In vitro...
While advancements in genome sequencing have identified millions of somatic mutations cancer, their functional impact is poorly understood. We previously developed the expression-based variant phenotyping (eVIP) method to use gene expression data characterize function variants. The eVIP uses a decision tree-based algorithm predict variants by comparing signatures induced introduction wild-type (WT) versus mutant cDNAs cell lines. distinguishes between that are gain-of-function,...
Abstract Oxidative stress caused by pregnancy‐induced hypertension syndrome significantly affects the health of pregnant women. Hydrogen sulfide is a typical gaseous signal molecule against oxidative stress, and it profound significance to develop detection method. In this study, stimuli‐responsive hydrogel was constructed based on coordination bonding principle metal ions chitosan (CS) realize quantitative hydrogen (H 2 S). The chain CS contains large number amino groups hydroxyl groups,...
Hydrogen sulfide (H2S) in joint fluid acts as a signal molecule to regulate inflammation. Direct detection of H2S is great significance for the diagnosis and treatment arthritis. However, due low volume concentration, existing methods face problem insufficient limit detection. In this study, highly sensitive biosensor was proposed by designing primer probe combining it with hybrid chain reaction (HCR) under strong interaction between metal ions achieve The containing multiple cytosine (C)...
Background Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive lethal malignancy. An effective prognosis prediction model urgently needed for treatment optimization. Methods The differentially expressed unfolded protein response (UPR)‒related genes between pancreatic tumor and normal tissue were analyzed using the TCGA-PDAC dataset, these that overlapped with UPR‒related prognostic from E-MTAB-6134 dataset further analyzed. Univariate, LASSO multivariate Cox regression analyses...
Abstract Purpose Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant tumors. Endoplasmic reticulum stress (ERS) plays an essential role in PDAC progression. Here, we aim to identify ERS-related genes and build reliable risk models for diagnosis, prognosis immunotherapy response patients as well investigate potential mechanism. Methods We obtained cohorts with transcriptional profiles clinical data from ArrayExpress, The Cancer Genome Atlas (TCGA) Genotype-Tissue Expression...
Abstract Colorectal cancer (CRC) is characterized by a complex tumor inflammatory microenvironment, while angiogenesis and immunosuppression frequently occur concomitantly. However, the exact mechanism that controls in CRC microenvironment remains unclear. Herein, we found expression levels of lipid raft protein STOML2 were increased associated with advanced disease stage poor survival outcomes. Intriguingly, revealed essential for which induces facilitates immune escape simultaneously both...