A5088786071- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Synthesis and biological activity
- Parasite Biology and Host Interactions
- Cytokine Signaling Pathways and Interactions
- Helminth infection and control
- CRISPR and Genetic Engineering
- Inflammasome and immune disorders
- Synthesis and Characterization of Heterocyclic Compounds
- Peptidase Inhibition and Analysis
- Bacterial biofilms and quorum sensing
- Heme Oxygenase-1 and Carbon Monoxide
- Protein Degradation and Inhibitors
- Galectins and Cancer Biology
- Polyamine Metabolism and Applications
- Muscle Physiology and Disorders
- SARS-CoV-2 and COVID-19 Research
- Coccidia and coccidiosis research
- Tryptophan and brain disorders
- Healthcare and Venom Research
- Plant and fungal interactions
- Lipid metabolism and biosynthesis
- Structural and Chemical Analysis of Organic and Inorganic Compounds
Tianjin Institute of Industrial Biotechnology
2023-2025
Chinese Academy of Sciences
2023-2025
Baylor College of Medicine
2017-2021
Southern California Institute for Research and Education
2020-2021
VA Long Beach Healthcare System
2020-2021
University of California, Irvine
2021
First Affiliated Hospital of Chengdu Medical College
2016
Guangdong Medical College
2016
Kakatiya University
1977-1979
Chaperone-mediated autophagy (CMA) is a specific form of that selectively targets proteins containing KFERQ-like motif and relies on the chaperone protein HSPA8/HSC70 for substrate recognition. In SERPINA1/a1-antitrypsin deficiency (AATD), disease characterized by hepatic buildup SERPINA1E342K/ATZ, CMA's role had been unclear. This work demonstrates critical CMA plays in preventing SERPINA1E342K/ATZ accumulation; suppressing worsens accumulation while activating it through chemical...
Inflammation contributes to the pathogenesis and morbidity of wide spectrum human diseases. The inflammatory response must be actively controlled prevent bystander damage tissues. Yet, mechanisms controlling excessive responses are poorly understood. NLRP3 inflammasome plays an important role in innate immune cellular infection or stress. Its activation tightly regulated because uncontrolled is associated with a number p38 MAPK signaling essential regulation inflammation. expression...
TFEB is a master regulator for transcription of genes involved in autophagy, lysosome and mitochondrial biogenesis. Activity inhibited upon its phosphorylation. STUB1, chaperone-dependent E3 ubiquitin ligase, modulates activity by preferentially targeting inactive phosphorylated degradation the proteasome pathway. Thus, ubiquitin-proteasome pathway participates regulating autophagy lysosomal functions TFEB.
Histone citrullination, an important post-translational modification mediated by peptidyl arginine deiminases, is essential for many physiological processes and epigenetic regulation. However, the causal relationship between histone citrullination specific gene regulation remains unresolved. In this study, we develop a programmable editor fusing deiminase PPAD from Porphyromonas gingivalis with dCas9. With assistance of gRNA, PPAD-dCas9 can recruit deiminases to genomic loci, enabling direct...
Protein citrullination involves the deimination of arginine or methylarginine residues in peptide chains to form citrulline by peptidyl deiminases. This process is an important protein post-translational modification that affects molecular structure and function various proteins, including histones. In recent years, has attracted widespread attention for its influence on gene transcription. Studies impact chromatin remodeling establishment regulatory networks have made rapid progress. this...
Alpha one antitrypsin (α1AT), a serine proteinase inhibitor primarily produced by the liver, protects pulmonary tissue from neutrophil elastase digestion. Mutations of SERPINA1 gene results in misfolded α1AT protein which aggregates inside hepatocytes causing cellular damage. Therefore, inhibition mutant production is practical strategy to alleviate liver Here we show that proteasome inhibitors can selectively downregulate expression human suppressing translation α1AT. Translational...
The α-1 antitrypsin Z-mutant protein (ATZ) is the primary cause of deficiency (AATD). Studying ubiquitination modification and degradation ATZ importance for developing treatments AATD. STUB1 an important E3 ubiquitin ligase that regulates various proteins. However, whether in involved has not been fully elucidated. In this study, coding genes were first cloned into pET28a plasmid, constructing 2 expression plasmids. recombinant plasmids then transferred
The spike (S) protein plays a crucial role in the entry of SARS-CoV-2 into host cells. S contains two subunits, S1 and S2. receptor-binding domain (RBD) subunit binds to receptor angiotensin-converting enzyme 2 (ACE2) enter Therefore, degrading is one feasible strategies inhibit infection. purpose this study develop degradation tool targeting S1. First, we constructed HEK 293 cell line stably expressing by using three-plasmid lentivirus system. overexpression mitochondrial E3 ubiquitin...
Objective To investigate microRNA (miRNA) expression profiles in peripheral blood and skin lesions of patients with psoriasis vulgaris (PV) , to seek miRNAs consistently expressed lesions. Methods A miRNA microarray was used screen differentially samples between 17 PV 4 healthy human controls, real-time fluorescence-based quantitative PCR (RT-qPCR) verify the miRNAs. The correlations these area severity index (PASI) score were assessed by Pearson correlation...
The control of cell growth, differentiation and apoptosis is realized through intercellular signal transduction dependent on activation signaling molecules. Abnormity in usually contributes to the occurrence diseases. Studies have demonstrated that psoriasis related multiple abnormal pathways, mainly including mitogenactivated protein kinase (MAPK)signaling pathway, nonreceptor tyrosine kinases Janus kinase/signal transducer activator transcription (JAK/STAT)pathway, tumor necrosis factor α...
SARS-CoV-2 relies on the recognition of spike protein by host cell receptor ACE2 for cellular entry. In this process, transmembrane serine protease 2 (TMPRSS2) plays a pivotal role, as it acts principal priming agent catalyzing cleavage to initiate fusion membrane with virus. Thus, TMPRSS2 is an ideal pharmacological target COVID-19 therapy development, and effective production high–quality essential basic research. Unfortunately, mammalian–originated protein, could not be solubly expressed...
Abstract Chaperone-mediated autophagy (CMA) is a specific form of that selectively targets proteins containing KFERQ-like motif and relies on the chaperone protein HSC70 for substrate recognition. In α1-antitrypsin deficiency (AATD), disease characterized by hepatic build-up α1-Antitrypsin Z mutant (ATZ), CMA’s role had been unclear. This work demonstrates critical CMA plays in preventing ATZ accumulation; suppressing worsens accumulation, whilst activating it through chemical stimulation or...
In this study, we showed that proteasome inhibitors selectively suppress the synthesis of Alpha One Antitrypsin (?1AT) by inducing phosphorylation eukaryotic translation initiation factor 2? and increasing association stress granule RNA binding proteins with ?1AT mRNA.