Fusarium infections, such as keratitis, are becoming increasingly difficult to control due the build-up of resistance fungi towards conventional antibiotics. Resistance is further enhanced by their ability form fungal biofilms. Poor storage conditions and inadequate cleaning contact lenses often lead corneal infections. The solution problems associated with contamination antimicrobials could lie in discovery new, affordable, efficacious antimicrobial compounds. purpose this study was...

The escalating global issue of food waste, valued at billions USD annually and significantly impacting sustainability across social, economic, environmental dimensions, necessitates innovative solutions to enhance waste management processes. Conventional decomposition techniques frequently encounter challenges related inefficiencies extended processing durations. This investigation examines the potential contributions medicinal plants as green catalysts in utilizing their bioactive compounds...

Endophytic fungi have the ability to live inside host plant tissues without causing neither symptoms of diseases/or harm. Opportunistic infections are accountable for majority outbreaks, thereby putting a burden on health system. To investigate and characterize bioactive compounds control bacteria clinical importance, extracts from endophytic were isolated indigenous South African medicinal plants. Extracts 133 fungal strains screened against Gram positive negative namely Bacillus cereus,...

To investigate the biodiversity of endophytic fungi isolated from indigenous Pelargonium sidoides plants using morphological and molecular techniques also screen isolates for antibacterial activities. In present study, a total 50 were collected roots analysed presence fungi. The initially sorted according to characteristics. Fungal identities further confirmed through amplification sequences encoding Translation Elongation Factor-1α (TEF-1α) gene Fusarium species Internal Transcribed Spacer...

Carbapenems are considered to be the last resort antibiotics for treatment of infections caused by extended-spectrum beta-lactamase (ESBL)-producing strains. The purpose this study was assess antimicrobial resistance profile Carbapenem-resistant

For centuries, spices have been utilized as flavourants, colourants and preservatives in food. Moreover, possess various antimicrobial properties with massive health benefits for the treatment management of ailments diseases. The present study was focused on three (3) aspects; (1) isolation molecular identification bacteria from meat; (2) to determine activity against pathogens; assess organoleptic spiced meat. A total twelve (n = 12) evaluated forty 40) spoilage food-borne pathogenic (

The aim of this study was to characterise Vibrio species water samples collected from taps, boreholes, and dams in the North West province, South Africa, assess biocontrol potentials their bacteriophages. Fifty-seven putative isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar identified using biochemical tests species-specific PCRs. Isolates further characterised based presence virulence factors, susceptibility eleven antibiotics, biofilm formation potentials. Twenty-two...

Endophytic microorganisms contribute significantly to water bioremediation by enhancing pollutant degradation and supporting aquatic plant health resilience releasing bioactive compounds enzymes. These inhabit tissues without causing disease or any noticeable symptoms. Endophytes effectively aid in eliminating contaminants from systems. Nanoparticles serve as potent enhancers processes, augmenting the efficiency of increasing surface area bioavailability, thereby improving efficacy rate...

Listeriosis is a foodborne disease caused by Listeria monocytogenes species and known to cause severe complications, particularly in pregnant women, young children, the elderly, immunocompromised individuals. The aim of this study was investigate presence food water using both biochemical species-specific PCR analysis. L. isolates were further screened for various antibiotic resistance, virulence, biofilm-forming determinants profiles phenotypic genotypic assays. A total 207 samples...

• A total of four hundred and ninety-two ( n =492) black seeds were utilized to isolate fungal strains. Both morphological molecular techniques used. PCR amplification Internal Transcribed Spacer (ITS 1 ITS 4) region. one =100) endophytic fungi successfully isolated with a 20% isolation rate. Salt-stress conditions, most isolates managed grow at 3% concentration, 58% the survived pH 2, 16% 37 °C. All belonged Ascomycota phylum being classified under Dothideomycetes (46%), Eurotiomycetes...

<ns3:p>As the global population is surging, agricultural industry required to meet food demand while simultaneously providing eco-friendly sustainable crops that can withstand numerous abiotic and biotic stresses. The current era requires high-throughput biotechnology approaches alleviate plant production protection crisis. Omics are regarded as a collection of high throughput technologies ending with “omics” such genomics, proteomics, transcriptomics, metabolomics, phenomics epigenomics....

Shiga toxin-producing Escherichia coli (STEC) strains have the potential to produce Shiga-toxins, which may lead enterohemorrhagic diarrhoea and kidney failure in individuals. Moreover, virulence genes of E. correlate their pathogenic nature causing severe morbidity mortality humans animals. Hemolytic-uremic syndrome (HUS), hemorrhagic colitis, might life-threatening hemolytic anemia, thrombocytopenia, acute renal failure. (E. coli) are well-known as gastrointestinal bacteria warm-blooded...

Forty-three (n = 43) endophytic fungi with different morphologic characteristics were from a medicinal plant Sceletium tortuosum, utilized to investigate their antifungal effectiveness against pathogenic fungi. All fungal isolates exhibited activity one or more pathogens in the dual culture test whereas only 33 filtrates (77%) showed decent effect. Fusaria and Aspergillus dominate genus that displayed significant activity. Isolates GG02, GG09, ND15, ND17 broadest spectrum of Furthermore,...

Current antimicrobial agents have become useless against multidrug-resistant pathogens. There is a necessity to discover novel compounds combat these The global trend promotes greener and more sustainable alternative, such as essential oils (EOs). EOs are complex volatile bioactive compounds, which assist plants in the defense pests A total of 14 were tested for antibacterial antifungal activity resistant thyme EO completely inhibits plant pathogenic fungi at all concentrations (100%...

Extracellular enzymes of the endophytes are degraders polysaccharides available in host plants. The study evaluated activity amylase, protease, lipase, and laccase produced by one hundred previously isolated from seed. In addition, their antimicrobial against Listeria monocytogenes, Staphylococcus aureus (ATTC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa 27853) was determined using disc diffusion assay. data show that 93% isolates were capable producing followed with 72%,...