A5072900473- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Antibiotic Resistance in Bacteria
- Microbial infections and disease research
- Listeria monocytogenes in Food Safety
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Food Safety and Hygiene
- Aquaculture disease management and microbiota
- Essential Oils and Antimicrobial Activity
- Clostridium difficile and Clostridium perfringens research
- Milk Quality and Mastitis in Dairy Cows
- Nanoparticles: synthesis and applications
- Fungal Biology and Applications
- Biochemical and Structural Characterization
- Nigella sativa pharmacological applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Diphtheria, Corynebacterium, and Tetanus
North-West University
2016-2025
University of Mpumalanga
2025
University of Nigeria
2023
Czech Academy of Sciences, Institute of Microbiology
2018
The increasing incidence of antibiotic resistance and emergence virulent bacterial pathogens, coupled with a lack new effective antibiotics, has reignited interest in the use lytic bacteriophage therapy. aim this study was to characterize
Green synthesis of nanoparticles using citrus peel extracts is known to be environmentally friendly and non-toxic when compared chemical methods. In this study, different obtained with the solvents acetone distilled water were used synthesize copper oxide (CuONPs). The characterized cyclic voltammetry, ultraviolet-visible spectroscopy, energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), scanning (SEM) Fourier-transform infrared (FTIR). absorption spectrum...
The aim of this study was to isolate Staphylococcus aureus from samples cow's milk obtained different farm settings and determine their antibiotic susceptibility patterns. Gram staining, oxidase, catalase, DNase, haemolysis the MASTASTAPHTM rapid agglutination tests were employed for bacterial identification. A total 28 collected screened presence S. aureus. All contaminated with 240 isolates during study. levels contamination higher in communal farms Lokaleng Mogosane (24.6% 35.4%,...
Extended spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae cause severe infections in humans which leads to complicated diseases. There is increasing evidence that cattle contribute the development and spread of multidrug resistant pathogens this raises public health concern. Despite this, data on concurrence ESBL cattle, especially North-West province are rare. Therefore, aim present study was isolate, identify characterise E. coli K. pneumoniae species from faeces raw beef...
Antimicrobial‐resistant Escherichia coli , especially those belonging to the serotype O157, are increasingly linked foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. O157:H7 due its ability produce toxins coupled low infectious dose. aim of this study was assess virulome, resistome, pathogenicity using whole genome sequencing. Three previously isolated strains from cattle feces were subjected sizes all three 5,117,276 bp,...
Contamination with Salmonella on food products and poultry in particular has been linked to foodborne infections and/or death humans. This study investigated the occurrence, genetic diversities antibiotic resistance profiles of strains isolated from chickens.Twenty each duplicate faecal swab samples were collected five different pens broilers, layers indigenous chickens North-West Province, South Africa. Isolates identities confirmed through amplification sequence analysis 16S rRNA invA gene...
Endophytic fungi have the ability to live inside host plant tissues without causing neither symptoms of diseases/or harm. Opportunistic infections are accountable for majority outbreaks, thereby putting a burden on health system. To investigate and characterize bioactive compounds control bacteria clinical importance, extracts from endophytic were isolated indigenous South African medicinal plants. Extracts 133 fungal strains screened against Gram positive negative namely Bacillus cereus,...
Pseudomonas aeruginosa has been implicated in a wide range of post-operation wound and lung infections. A acquired resistance virulence markers indicate surviving strategy P. aeruginosa. Complete-genome analysis identified as efficient approach towards understanding the pathogenicity this organism. This study was designed to sequence entire genome UY1PSABAL UY1PSABAL2; determine drug-resistance profiles factors isolates; assess that contribute toward stability genomes; thereafter...
Listeria monocytogenes (Lm) is the causative agent of human listeriosis. Lm strains have different virulence potential. For this reason, we preliminarily characterised via Whole-Genome Sequencing (WGS) some for their key genomic features and virulence-associated determinants, assigning clonal complex (CC). Moreover, ability same to adhere invade colon carcinoma cell line Caco-2, evaluating possible correspondence with genetic profile, was also assessed. The clinical typed belonged (CC)1,...
Abstract This study aimed to compare the spectroscopy, morphological, electrocatalytic properties, and antibacterial activities of cobalt nanoparticles (CoNPs) with nickel (NiNPs). Cobalt NiNPs were prepared via a chemical reduction approach characterized utilizing transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), diffraction (XRD) techniques. The result from XRD TEM analysis revealed that synthesized exhibit face-centered cubic smooth spherical shape, having average...
The misuse/abuse of antibiotics in intensive animal rearing and communities led to the emergence resistant isolates such as vancomycin-resistant enterococci (VREs) worldwide. This has become a major source concern for public health sector. aim this study was report antibiotic resistance profiles highlight presence virulence genes VREs isolated from feedlots cattle North-West Province South Africa. 384 faecal samples, 24 drinking troughs water, soil samples were collected aseptically 6...
Thirty-three (33) isolates of methicillin-resistant Staphylococcus aureus (MRSA) from healthy edible marine fish harvested two aquaculture settings and the Kariega estuary, South Africa, were characterised in this study. The phenotypic antimicrobial susceptibility profiles to 13 antibiotics determined, their antibiotic resistance determinants assessed. A multiplex PCR was used determine epidemiological groups based on type SCC mec carriage followed by detection staphylococcal...
For centuries, spices have been utilized as flavourants, colourants and preservatives in food. Moreover, possess various antimicrobial properties with massive health benefits for the treatment management of ailments diseases. The present study was focused on three (3) aspects; (1) isolation molecular identification bacteria from meat; (2) to determine activity against pathogens; assess organoleptic spiced meat. A total twelve (n = 12) evaluated forty 40) spoilage food-borne pathogenic (
In many developing countries, proper hygiene is not strictly implemented when animals are slaughtered and meat products become contaminated. Contaminated may contain Escherichia coli (E. coli) O157:H7 that could cause diseases in humans if these food consumed undercooked. the present study, a total of 94 confirmed E. isolates were subjected to enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR) typing generate genetic fingerprints. The ERIC fragments...
Atypical enteropathogenic E. coli (aEPEC) is a group of diarrhoeagenic Escherichia with high diversity serotypes, which lack the bundle-forming pili (BFP) and genes encoding for shiga toxins. The aim this study was to isolate, identify determine virulence antibiotic resistance profiles aEPEC O177 strains from cattle faeces. A total 780 samples were collected beef dairy analysed presence O177. One thousand two hundred seventy-two (1272) presumptive isolates obtained 915 confirmed as species....