A5021608443- Chromatin Remodeling and Cancer
- Intraocular Surgery and Lenses
- Coastal and Marine Management
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- Digital Imaging in Medicine
- Patient Satisfaction in Healthcare
- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Mathematical Biology Tumor Growth
- Retinal Imaging and Analysis
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- Healthcare Operations and Scheduling Optimization
- DNA Repair Mechanisms
Henry Ford Health System
2023
University of Michigan
2019-2022
Institute of Human Genetics
2018
Neuroscience Institute
2018
Abstract The brain is a genomic mosaic shaped by cellular responses to genome damage. Here, we manipulate somatic stability conditional Knl1 deletion from embryonic mouse brain. KNL1 mutations cause microcephaly and mediates the spindle assembly checkpoint, safeguard against chromosome missegregation aneuploidy. We find that following deletion, segregation errors in mitotic neural progenitor cells give rise DNA damage on missegregated chromosomes. This triggers rapid p53 activation robust...
Abstract Chromatin regulates spatiotemporal gene expression during neurodevelopment, but it also mediates DNA damage repair essential to proliferating neural progenitor cells (NPCs). Here, we uncover molecularly dissociable roles for nucleosome remodeler Ino80 in chromatin-mediated transcriptional regulation and genome maintenance corticogenesis. We find that conditional deletion from cortical NPCs impairs double-strand break (DSB) repair, triggering p53-dependent apoptosis microcephaly....
Cataract surgery is one of the most commonly performed surgeries across medicine and an integral part ophthalmologic care. Complex cataract requires more time resources than simple surgery, yet it remains unclear whether incremental reimbursement for complex compared with offsets increased costs.
Significance The cognitive, perceptive, and motor capabilities of the mammalian cerebral cortex depend on assembly circuit connectivity during development. Subplate neurons, strategically located at junction gray white matter, orchestrate wiring cortical circuits. Using a genetic approach to study gene necessity sufficiency in subplate we uncover an essential role for chromatin remodeler Arid1a neuron expression axon guidance functions. Cortical deletion disrupts subplate-dependent formation...
To develop a method for accurate automated real-time identification of instruments in cataract surgery videos.Cataract videos were collected at University Michigan's Kellogg Eye Center between 2020 and 2021. Videos annotated the presence to aid development, validation, testing machine learning (ML) models multiclass, multilabel instrument identification.A new database, BigCat, was assembled, containing 190 with over 3.9 million frames, largest reported annotation database date. Using dense...
Abstract Loss-of-function mutations in chromatin remodeler gene ARID1A are a cause of Coffin-Siris syndrome, developmental disorder characterized by dysgenesis corpus callosum. Here, we characterize Arid1a function during cortical development and find unexpectedly selective roles for subplate neurons. Subplate neurons (SPNs), strategically positioned at the interface grey white matter, orchestrate multiple processes indispensable neural circuit wiring. We that pan-cortical deletion leads to...