A cyclic elasto-plastic constitutive model based on a non-linear kinematic hardening rule for sand is proposed. Three points are incorporated into the model: new flow rule, cumulative strain-dependent characteristic of plastic shear modulus and fading memory initial anisotropy model. In order to verify its effectiveness, proposed was evaluated by means results series hollow-cylinder torsional tests with without an stress after isotropic anisotropic consolidation. The liquefaction strength...

Abstract α-l-Arabinofuranosidase (α-l-arafase) was purified from fruit of Japanese pear (Pyrus pyrifolia). The enzyme solubilized the cell wall by NaCl and Triton X-100 had homogeneity a single 62-kD polypeptide on SDS-PAGE after purification through steps hydroxyapatite, anion-exchange chromatography, size-exclusion chromatography. A related cDNA clone isolated (PpARF2). transcript protein were detected solely in ripening corresponding to increase α-l-arafase activity. Transcripts PpARF2...

We have cloned a cDNA fragment encoding β-galactosidase from Japanese pear (Pyrus pyrifolia) fruit (JP-GAL). It contained an untranslated sequence of 182 nucleotides at the 5′ end, presumptive coding 2,193 and 268 including polyadenylation signal poly (A) tail 3′ end. encoded protein with calculated molecular weight 80.9 kDa which consists 731 amino acids. Both nucleotide deduced acid sequences showed 98% identity that obtained apple cDNA. The peptide purified III matched...

Fruit softening and textural changes are two important factors of fruit quality. The loss galactosyl arabinosyl residues from cell wall polysaccharides is observed in many species during ripening. release neutral sugar could change properties accessibility or reactivity for other hydrolases. β-Galactosidase α-L-arabinofuranosidase contribute to the residues. Thus, enzymes alter polysaccharide might composed multiple isozymes they clearly distinguishable by their substrate specificity...

Membranous lipodystrophy apparently is a new disease, first reported and named by Nasu associates 31 in 1971. We have collected six cases of this disease. The initial manifestation was articular pain at adolescence. Symmetrical changes the bones extremities then appeared disease seemed to progress slowly with age. younger patients exhibited only skeletal or fractures, while older had some neuropsychiatric changes. Three died middle cystic bone lesions contained yellow, lipid-like substance...

Azole resistance of Aspergillus fumigatus isolates has been reported worldwide and it would appear to be mainly due a point mutation in the 14α-sterol demethylase (CYP51A) gene, which is target enzyme for azoles. The confirmed from patients who received long-term itraconazole (ITZ) therapy agricultural fields where high levels azole fungicides were employed. However, relationship between farm environments azole-resistant A. not fully studied. In this investigation, 50 obtained tetraconazole...

Based on the dynamic substructure technique, author proposes a static seismic analysis method, Ground Response Method, for underground structures modeled structure and surrounding soil that is expressed using finite elements. By comparing existing methods, it verified Acceleration Method Buried Structures provides results almost equivalent to those obtained from Method. However, FEM Seismic Deformation yields different results. Thus, new load ground strains modifies

Ripening of climacteric fruits is initiated when the gaseous plant hormone ethylene perceived by cell. Ethylene binding to membrane-associated receptors (ETRs) triggers a series biochemical events through multiple components, resulting in induction numerous ripening-related genes. In tomato (Solanum lycopersicum L.), there are seven members ETR family, which each contribute regulation fruit ripening. However, relative contribution individual receptor signaling remains unknown. Here, we...

Substantial decreases in cell wall bound galactosyl and arabinosyl residues are two of the most evident compositional changes that occur during fruit ripening. The roles β-galactosidase (β-Gal) α-L-arabinofuranosidase (α-Af), enzymes responsible for these respective losses, were investigated compared European (Pyrus communis L. 'La France') Chinese bretschneideri Rehd. 'Yali') pear fruits which exhibit different softening characteristics increase activities β-Gal α-Af ripening both types...

Four cDNA clones (SlArf/Xyl1-4) encoding α-l-arabinofuranosidase/β-xylosidase belonging to glycoside hydrolase family 3 were obtained from tomato (Solanum lycopersicum) fruit. SlArf/Xyl1 was expressed in various organs. Its level particularly high flower and leaves but low SlArf/Xyl3 highly flower. On the contrary, SlArf/Xyl2 4 early developmental stage Comparison with SlArf/Xyl4, expression observed earlier stages. The active recombinant proteins by using BY-2 tobacco (Nicotiana tabacum)...

Galactosidases are thought to play a key role in cell wall metabolism during fruit growth and ripening. In this study we cloned seven β-galactosidase (β-Gal) cDNAs from japanese pear designated them PpGAL2, PpGAL3, Pp-GAL4, PpGAL5, PpGAL6, PpGAL7 , PpGAL8 addition the previously described JP-GAL hereinafter termed PpGAL1 . mRNA expression patterns of these clones were characterized throughout on-tree ripening, leaves shoots three cultivars, `Housui', `Kousui', `Niitaka'. The shared amino...

Transport of water into cells is mediated by plasma membrane intrinsic protein (PIP) families aquaporin, which are involved in petal cell expansion during flower opening. In this study, we performed comprehensive characterization aquaporin family genes and analyzed the expression PIP petals opening flowers to examine role PIPs carnation (Dianthus caryophyllus L.). A database search genome sequence revealed existence 26 with 8 members subfamily 'Francesco'. The all was validated expressed...

Since there is little information about the relationship between glycosidases and avocado fruit softening, we assayed some glycosidase activities during softening. β-Galactosidase, α-L-arabinofuranosidase β-glucosidase increased ripening ; that of β-galactosidase activity closely paralleled trend in Therefore, was fractionated into three isoforms using hydrophobic chromatography cation exchange were labeled AV-GAL I, II or III. III fruit, which detected 4 days after harvest, with whereas I...

"Flavonoids in the Extract and Exudate of Roots Leguminous Crops." Plant Production Science, 4(4), pp. 278–279Key words: FlavonoidLeguminous cropRoot extractRoot exudate

地中構造物の地震時振動特性に基づき, 動的サブストラクチャー法の運動方程式より周辺地盤をFEMで表した静的耐震計算法 (地盤応答法) を導いた. 地盤応答法と既往の静的耐震計算法を比較したところ, 応答震度法は主に自然地盤の加速度による慣性力を作用させるという点で地盤応答法と似通った計算法であるが, 地盤FEM型の応答変位法は自然地盤の地震時変位を近傍地盤外周に作用させるという点で地盤応答法とは異なる計算法であることが判明した.

cDNA clone encoding a β-galactosidase, AV-GAL, was isolated from fruit of avocado (Persea americana Mill) in which both nucleotide and deduced amino acid sequences had about 70 % identity with those other plant β-galactosidases. The calculated molecular mass the presumptive protein, consists 766 acids, 85.8 kDa. It contained putative active site consensus sequence, G-G-P-[LIVM](2)-x(2)-Q-x-E-N-E-[FY] (amino 182-194), belonging to glycosyl hydrolase family 35 signal peptide would be cleaved...

A mixture of xyloglucan oligosaccharides (XGO) was prepared from (XG) tamarind seed gum by digestion with Aspergillus recombinant XG-specific xyloglucanase and subsequent purification ethanol fractionation. The XGO contained XG7, XG8, XG9 at the ratio 1 : 4 5, which almost identical to literature value constituent subunits 1.2 3.8 5. 1% promoted flower opening in carnation (Dianthus caryophyllus L.) cultivars, such as 'Pure Red' 'Lillian'. On other hand, there no effect on 'Collin', 'Light...

Malignant transformation of neurofibromatosis is one the most serious complications von Recklinghausen's disease (VRD). The common associated malignancy malignant peripheral nerve sheath tumour (MPNST). Few cases MPNST with VRD in maxillary region have been reported. This report describes a rare case maxilla and aggressive nature VRD.

We investigated changes in starch content and metabolic enzyme activities developing postharvest squash of distinct species, Cucurbita maxima moschata, which accumulate high low levels starch, respectively. The total activity phosphorylase fruits significantly correlated (r = 0.99) to the amount among species (C. maxima, C. moschata pepo). Separable a plastidial L-form fruit markedly increased corresponding with accumulation. isolated two genes (CmPhoL1 CmPhoH1) encoding an cytosolic H-form...