A5024174094- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Redox biology and oxidative stress
- Genomics, phytochemicals, and oxidative stress
- Heat shock proteins research
- Plant Molecular Biology Research
- S100 Proteins and Annexins
- Light effects on plants
- Plant-derived Lignans Synthesis and Bioactivity
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Bioenergy crop production and management
- Traditional and Medicinal Uses of Annonaceae
- Glutathione Transferases and Polymorphisms
- Polyamine Metabolism and Applications
- Plant Parasitism and Resistance
- Signaling Pathways in Disease
- Peroxisome Proliferator-Activated Receptors
- Plant responses to water stress
- Metal-Catalyzed Oxygenation Mechanisms
- ATP Synthase and ATPases Research
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Trace Elements in Health
Bielefeld University
2010-2024
The jasmonate family of phytohormones plays central roles in plant development and stress acclimation. However, the architecture their signaling circuits remains largely unknown. Here we describe a binding protein, cyclophilin 20-3 (CYP20-3), which regulates stress-responsive cellular redox homeostasis. (+)-12-oxo-phytodienoic acid (OPDA) promotes CYP20-3 to form complex with serine acetyltransferase 1, triggers formation hetero-oligomeric cysteine synthase O -acetylserine(thiol)lyase B...
Chloroplasts are equipped with a nuclear-encoded antioxidant defence system the components of which usually expressed at high transcript and activity levels. To significantly challenge chloroplast system, Arabidopsis thaliana plants, acclimated to extremely low light slightly above compensation point or normal growth chamber light, were moved corresponding 100- 10-fold jump, for 6 h 24 in order observe responses water–water cycle transcript, protein, enzyme activity, metabolite The plants...
The integration of redox- and reactive oxygen species-dependent signaling metabolic activities is fundamental to plant acclimation biotic abiotic stresses. Previous data suggest the existence a dynamically interacting module in chloroplast stroma consisting cyclophilin 20-3 (Cyp20-3), O-acetylserine(thiol)lyase B (OASTL-B), 2-cysteine peroxiredoxins A/B (2-CysPrx) serine acetyltransferase 2;1 (SERAT2;1). functionality this COPS influenced by redox stimuli oxophytodienoic acid (OPDA), which...
cis-(+)-12-Oxophytodienoic acid (OPDA) is a reactive oxylipin produced by catalytic oxygenation of polyunsaturated α-linolenic (18:3 (ω - 3)) in the chloroplast. Apart from its function as precursor for jasmonic synthesis, OPDA serves signaling molecule and regulator on own, namely tuning enzyme activities altering expression OPDA-responsive genes. A possible reaction mechanism covalent binding to thiols via addition C=C double bond α,β-unsaturated carbonyl group cyclopentenone ring. The...
2-Cysteine peroxiredoxins (2-CysPrxs) switch between functions as a thiol peroxidase, chaperone, an interaction partner and possibly proximity-based oxidase in redox-dependent manner. In photosynthetic eukaryotes, 2-CysPrx localizes to the plastid, context of photosynthesis enables ascorbate peroxidase-independent water–water cycle for detoxifying H2O2. The high degree evolutionary conservation suggests that switching is essential characteristic needed transduce redox information downstream...
α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) abscisic (ABA) conjugated cysteine. It was found that the GSH followed sequence trans-2-hexenal < 12-OPDA ≈ 12-OPDA-ethylester 2-cyclopentenone << methyl vinylketone (MVK). Interestingly, GSH, but not...
13-lipoxygenases (13-LOX) catalyze the dioxygenation of various polyunsaturated fatty acids (PUFAs), which α-linolenic acid (LeA) is converted to 13-S-hydroperoxyoctadeca-9, 11, 15-trienoic (13-HPOT), precursor for prostaglandin-like plant hormones cis-(+)-12-oxophytodienoic (12-OPDA) and methyl jasmonate (MJ). This study aimed characterizing four annotated A. thaliana 13-LOX enzymes (LOX2, LOX3, LOX4, LOX6) focusing on synthesis 12-OPDA 4Z,7Z,10Z)-12-[[-(1S,5S)-4-oxo-5-(2Z)-pent-2-en-1yl]...
Brassica juncea annexin-3 (BjAnn3) was functionally characterized for its ability to modulate H2O2-mediated oxidative stress in Saccharomyces cerevisiae. BjAnn3 showed a significant protective role cellular-defense against and partially alleviated inhibition of mitochondrial respiration presence exogenously applied H2O2. Heterologous expression protected membranes from stress-mediated damage positively regulated antioxidant gene ROS detoxification. We conclude that, counteracts the effects...
Plants are often simultaneously infested by several herbivores at the shoots and roots. Recent results revealed that model plant Arabidopsis thaliana shows highly challenge-specific local systemic responses to individual simultaneous attacks of shoot-infesting aphids root-infesting nematodes metabolome level. (1) Here, we present corresponding transcriptional changes in plants treated with Brevicoryne brassicae Heterodera schachtii individually combination. Overall, were much less responsive...