A5101494989- Herpesvirus Infections and Treatments
- interferon and immune responses
- Virus-based gene therapy research
- Web Data Mining and Analysis
- RNA regulation and disease
- Toxin Mechanisms and Immunotoxins
- Semantic Web and Ontologies
- Data Quality and Management
- Advanced Database Systems and Queries
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Vectors
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Autophagy in Disease and Therapy
- Heat shock proteins research
- Data Management and Algorithms
- Microtubule and mitosis dynamics
- Viral Infections and Immunology Research
- Algorithms and Data Compression
- Cytomegalovirus and herpesvirus research
- Web visibility and informetrics
- Toxoplasma gondii Research Studies
- Inflammasome and immune disorders
- Data Mining Algorithms and Applications
- MicroRNA in disease regulation
University of Illinois Urbana-Champaign
2011-2023
University of Illinois Chicago
2012-2023
Jiangxi Science and Technology Normal University
2023
Hunan University
2022-2023
First People's Hospital of Chongqing
2022
Shanghai Chest Hospital
2021-2022
Shanghai Jiao Tong University
2021-2022
Illinois College
2008-2021
Zhengzhou University
2021
Chenzhou First People's Hospital
2021
In human cells infected with herpes simplex virus 1 the double-stranded RNA-dependent protein kinase (PKR) is activated but phosphorylation of α subunit eukaryotic translation initiation factor 2 (eIF-2) and total shutoff synthesis observed only in γ z34.5 − mutants. The carboxyl-terminal 64 aa 34.5 are homologous to corresponding domain MyD116, murine growth arrest DNA damage gene 34 (GADD34) two domains functionally interchangeable cells. This report shows that ( i ) carboxyl terminus...
The Web has been rapidly "deepened" by the prevalence of databases online. With potentially unlimited information hidden behind their query interfaces, this "deep Web" searchable databses is clearly an important frontier for data access. This paper surveys relatively unexplored frontier, measuring characteristics pertinent to both exploring and integrating structured sources. On one hand, our "macro" study deep at large, in April 2004, adopting random IP-sampling approach, with million...
The jasmonate family of phytohormones plays central roles in plant development and stress acclimation. However, the architecture their signaling circuits remains largely unknown. Here we describe a binding protein, cyclophilin 20-3 (CYP20-3), which regulates stress-responsive cellular redox homeostasis. (+)-12-oxo-phytodienoic acid (OPDA) promotes CYP20-3 to form complex with serine acetyltransferase 1, triggers formation hetero-oligomeric cysteine synthase O -acetylserine(thiol)lyase B...
Schema matching is a critical problem for integrating heterogeneous information sources. Traditionally, the of multiple schemas has essentially relied on finding pairwise-attribute correspondence. This paper proposes different approach, motivated by large numbers data sources Internet. On this "deep Web," we observe two distinguishing characteristics that offer new view considering schema matching: First, as Web scales, there are ample provide structured in same domains (e.g., books and...
Recently, the Web has been rapidly "deepened" by many searchable databases online, where data are hidden behind query forms. For modelling and integrating databases, very first challenge is to understand what a interface says- or capabilities source supports. Such automatic extraction of semantics challenging, as forms created autonomously. Our approach builds on observation that, across myriad sources, seem reveal some "concerted structure," sharing common building blocks. Toward this...
Enterovirus 71 (EV71) is a human pathogen that induces hand, foot, and mouth disease fatal neurological diseases. Immature or impaired immunity thought to associate with increased morbidity mortality. In murine model, EV71 does not facilitate the production of type I interferon (IFN) plays critical role in first-line defense against viral infection. Administration neutralizing antibody IFN-alpha/beta exacerbates virus-induced disease. However, molecular events governing this process remain...
TANK-binding kinase 1 (TBK1) is a key component of Toll-like receptor-dependent and -independent signaling pathways. In response to microbial components, TBK1 activates interferon regulatory factor 3 (IRF3) cytokine expression. Here we show that novel target the gamma(1)34.5 protein, virulence whose expression regulated in temporal fashion. Remarkably, protein required inhibit IRF3 phosphorylation, nuclear translocation, induction antiviral genes infected cells. When expressed mammalian...
Significance The innate immune system plays a key role in host defense that involves the detection of microbial components and series signaling events lead to production interferons cytokines. Recently, identification mitochondrial antiviral-signaling (MAVS) protein placed mitochondria at forefront response against virus infection. However, how MAVS complex is assembled regulated on outer membrane only partially understood. Here we show tripartite motif 14 (TRIM14) facilitates assembly...
The carboxyl-terminal domain of the γ<sub>1</sub>34.5 protein herpes simplex virus 1 binds to phosphatase 1α (PP1) and is required prevent shut-off synthesis resulting from phosphorylation α subunit eIF-2 by double-stranded RNA-activated kinase. corresponding conserved GADD34 homologous functionally substitutes for γ<sub>1</sub>34.5. This report shows that PP1 form a complex in infected cells, fractions containing this specifically dephosphorylate eIF-2α, both proteins contain amino acid...
Heat shock transcription factor 1 (HSF-1) activates the of heat genes in eukaryotes. Under normal physiological growth conditions, HSF-1 is a monomer. Its transcriptional activity repressed by constitutive phosphorylation. Upon activation, forms trimers, acquires DNA binding activity, increases and appears as punctate granules nucleus. In this study, using bromouridine incorporation confocal laser microscopy, we demonstrated that newly synthesized pre-mRNAs colocalize to after shock,...
The gamma(1)34.5 protein of herpes simplex virus (HSV) plays a crucial role in infection. Although the double-stranded RNA-dependent kinase (PKR) is activated during HSV infection, inhibits activity PKR by mediating dephosphorylation translation initiation factor eIF-2alpha. Here we show that infection also induces phosphorylation an endoplasmic reticulum (ER) resident PERK, hallmark ER stress response. virus-induced PERK blocked cycloheximide but not phosphonoacetic acid, suggesting...
The NOVH protein belongs to the emerging CCN [Connective tissue growth factor (CTGF), Cyr61/Cef10, nephroblastoma overexpressed gene] family of regulators sharing a strikingly conserved multimodular organization but exhibiting distinctive functional features. Two members (CYR61 and CTGF) are positive cell proliferation, whereas two other (ELM1 RCOP-1) exhibit features negative growth. structure these proteins suggests that their biological role(s) may depend on interactions with several...
To enable information integration, schema matching is a critical step for discovering semantic correspondences of attributes across heterogeneous sources. While complex matchings are common, because their far more search space, most existing techniques focus on simple 1:1 matchings. tackle this challenge, paper takes conceptually novel approach by viewing as correlation mining, our task Web query interfaces to integrate the myriad databases Internet. On "deep Web," generally form between...
The herpes simplex virus 1 mutants from which both copies of the gamma(1)34.5 gene had been deleted trigger total shutoff protein synthesis in human neuroblastoma cells and foreskin fibroblasts but not African green monkey (Vero) cells. carboxyl-terminal 64 amino acids are homologous to corresponding domain MyD116, a murine myeloid differentiation primary responsive gene. is required preclude (J. Chou B. Roizman, Proc. Natl. Acad. Sci. USA 91:5247-5251, 1994). We report that in-frame...
The mammalian heat shock transcription factor HSF-1 regulates the expression of proteins, molecular chaperones that are involved in cellular processes from higher order assembly to protein degradation. is a phosphorylated monomer under physiological growth conditions and located mainly cytoplasm. Upon activation by variety environmental stresses, translocated into nucleus, forms trimers, acquires DNA binding activity, hyperphosphorylated, appears as punctate granules, increases...
Earlier studies have shown that infection of human cells by herpes simplex virus 1 (HSV-1) results in the activation RNA-dependent protein kinase (PKR) but alpha subunit eIF-2 is not phosphorylated and synthesis unaffected. In absence viral gamma(1)34.5 gene, eIF-2alpha prematurely shut off (J. Chou, J. Chen, M. Gross, B. Roizman, Proc. Natl. Acad. Sci. USA 92:10516-10520, 1995). A second recent paper reported selection second-site suppressor mutants characterized near-wild-type infected...
Long-term survivors of glioblastoma multiforme, the most common form primary intracranial malignancy in adults, are extremely rare. Experimental animal models that more closely resemble human disease essential for identification effective novel therapies. We report here an extensive analysis 4C8 glioma model to assess its suitability evaluating type 1 herpes simplex virus (HSV-1) therapies malignant glioma. first determined expression major histocompatibility complex I and II αvβ3 was...
The ICP34.5 protein of herpes simplex virus type 1 is a neurovirulence factor that plays critical roles in viral replication and anti-host responses. One its functions to recruit phosphatase (PP1) leads the dephosphorylation α subunit translation initiation eIF2 (eIF2α), which inactivated by infection-induced phosphorylation. As PP1 with wide range substrates, question remains be answered how directs specifically dephosphorylate eIF2α. Here we report not only binds but also associates eIF2α...
Autophagy has been established as a player in host defense against viruses. The mechanisms by which the induces autophagy during infection are diverse. In case of HSV type 1 (HSV-1), dsRNA-dependent protein kinase is essential for induction fibroblasts through phosphorylation eukaryotic initiation factor 2α (eIF2α). HSV-1 counteracts via ICP34.5, dephosphorylates eIF2α and inhibits Beclin 1. Investigation largely conducted permissive cells, but recent work suggests existence...
Viral inhibition of innate immunity contributes to herpes simplex virus pathogenesis. Although this complex process involves multiple factors, the underlying events remain unclear. We demonstrate that an HSV virulence factor γ 1 34.5 precludes activation STING, a central adaptor in intracellular DNA sensing pathway. Upon infection, viral protein engages with and inactivates STING. Consequently, it compromises host facilitates replication. These observations uncover mechanism is likely...
To enable information integration, schema matching is a critical step for discovering semantic correspondences of attributes across heterogeneous sources. While complex matchings are common, because their far more search space, most existing techniques focus on simple 1:1 matchings. tackle this challenge, article takes conceptually novel approach by viewing as correlation mining , our task Web query interfaces to integrate the myriad databases Internet. On “deep ” generally form between...