A5060207084- Phytochemicals and Antioxidant Activities
- Biochemical and biochemical processes
- Listeria monocytogenes in Food Safety
- Microbial Metabolic Engineering and Bioproduction
- Microbial Inactivation Methods
- Enzyme Catalysis and Immobilization
- Fermentation and Sensory Analysis
- Meat and Animal Product Quality
- Protease and Inhibitor Mechanisms
- Antioxidant Activity and Oxidative Stress
- Polysaccharides Composition and Applications
- Enzyme Production and Characterization
- Diet and metabolism studies
- Electrochemical sensors and biosensors
- Tryptophan and brain disorders
- Vitamin C and Antioxidants Research
- Aquaculture disease management and microbiota
- Polysaccharides and Plant Cell Walls
- Biofuel production and bioconversion
- Essential Oils and Antimicrobial Activity
- Heat shock proteins research
- Food Quality and Safety Studies
- Plant biochemistry and biosynthesis
- Silk-based biomaterials and applications
- Cholesterol and Lipid Metabolism
Shaanxi Normal University
2016-2025
Ministry of Education of the People's Republic of China
2023-2025
University of California, Los Angeles
2011-2021
China Agricultural University
2011-2020
Ocean University of China
2016
Sichuan University
2013
Chengdu Medical College
2013
The Barbara Ann Karmanos Cancer Institute
2005
Wayne State University
2005
Augusta University
1997-1999
We performed silencing and overexpression studies of flavin containing monooxygenase (FMO) 3 in hyperlipidemic mouse models to examine its effects on trimethylamine N-oxide (TMAO) levels atherosclerosis. Knockdown hepatic FMO3 LDL receptor knockout mice using an antisense oligonucleotide resulted decreased circulating TMAO Surprisingly, we also observed significant decreases lipids plasma lipids, ketone bodies, glucose, insulin. transgenic mice, the other hand, increased lipids. Global gene...
Emerging evidence suggests that microbes resident in the human intestine represent a key environmental factor contributing to obesity-associated disorders. Here, we demonstrate gut microbiota-initiated trimethylamine N-oxide (TMAO)-generating pathway is linked obesity and energy metabolism. In multiple clinical cohorts, systemic levels of TMAO were observed strongly associate with type 2 diabetes. addition, circulating associated traits different inbred strains represented Hybrid Mouse...
Heat shock transcription factor 1 (HSF-1) activates the of heat genes in eukaryotes. Under normal physiological growth conditions, HSF-1 is a monomer. Its transcriptional activity repressed by constitutive phosphorylation. Upon activation, forms trimers, acquires DNA binding activity, increases and appears as punctate granules nucleus. In this study, using bromouridine incorporation confocal laser microscopy, we demonstrated that newly synthesized pre-mRNAs colocalize to after shock,...
Fatty alcohols are important oleochemicals widely used in detergents, surfactants and personal care products. Bio-synthesized fatty alcohol provides a promising alternative to traditional industry. Harnessing oleaginous microorganisms for production may offer new strategy achieve commercially viable yield that currently still seems be remote target.In this study, we introduced functional acyl-CoA reductase (FAR), TaFAR1 direct the conversion from Yarrowia lipolytica (Y. lipolytica), an...
Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, solution aid, would reduce productivity of enzyme. Esterification, solvent-free no-glycerol-release system for production, developed, two-step catalysis soybean oil, hydrolysis followed esterification, with Yarrowia lipolytica lipase is reported this paper.First, oil was hydrolyzed at 40°C 100 U broth...
Lipocalin-2 (LCN2) is a secreted protein involved in innate immunity and has also been associated with several cardiometabolic traits both mouse human studies. However, the causal relationship of LCN2 to these unclear, most studies have examined only males. Using adeno-associated viral vectors we expressed either adipose or liver tissue specific manner on background whole-body Lcn2 knockout wildtype mice. Metabolic phenotypes including body weight, composition, plasma lipids, glucose...
The activation of heat shock transcription factor-1 (HSF-1) after treatment mammalian cells with stresses such as shock, heavy metals, or ethanol induces the synthesis proteins. HSF-1 is phosphorylated at normal growth temperature and hyperphosphorylated upon stress. We recently presented evidence that can be by mitogen activated protein kinase, ERK1, phosphorylation appears to negatively regulate activity HSF-1. In this report, we have tested ability ERK1 phosphorylate various deletion...
Abstract It is well documented that tumor suppressive maspin inhibits cell invasion and extracellular matrix remodeling. Maspin a cytosolic, surface–associated, secreted protein in the serine protease inhibitor superfamily. Although several molecules have been identified as candidate intracellular targets, target(s) remains elusive. does not directly inhibit urokinase-type plasminogen activator (uPA) activity, we shown evidence may block pericellular proteolysis mediated by...
A growth trial was conducted to evaluate the effects and safety of nucleotides in low fish meal diets on performance, antioxidative capacity intestinal morphology turbot (Scophthalmus maximus). High control diet formulated with 500 g kg−1 meal. Seven levels (0.075, 0.15, 0.225, 0.300, 1.5 3.0 kg−1, respectively) were added a basal diet, which 400 The eight experimental fed groups juvenile (initial weight: 6.0 ± 0.03 g) for 60 days. Results showed that compared high treatment had lower total...
Aromatic aldehydes find extensive applications in food, perfume, pharmaceutical, and chemical industries. However, a limited natural enzyme selectivity has become the bottleneck of bioconversion aromatic from phenylpropanoid acids. Here, based on original structure feruloyl-coenzyme A (CoA) synthetase (FCS) Streptomyces sp. V-1, we engineered five substrate-binding domains to match specific FcsCIAE407A/K483L, FcsMAE407R/I481R/K483R, FcsHAE407K/I481K/K483I, FcsCAE407R/I481R/K483T,...
One-pot biosynthesis of vanillin from ferulic acid without providing energy and cofactors adds significant value to lignin waste streams. However, naturally evolved carotenoid cleavage oxygenase (CCO) with extreme catalytic conditions greatly limited the above pathway for bioproduction. Herein, CCO
Hyperuricemia (HUA) is a globally prevalent metabolic disorder characterized by an imbalance in uric acid (UA) production and excretion. In this study, we found that oleanolic (OA), natural pentacyclic triterpene, effectively reduced HUA associated kidney injury C57BL/6J mice. A 12-week OA treatment significantly dose-dependently UA creatinine levels serum urine while suppressing hepatic xanthine oxidase activity Mechanistic analysis revealed modulates the expression of urate transporters...
Enterolignan is a vital anticancer compound, and benzyl ether reductase (BER) plays key role in its biosynthesis by facilitating lignan biotransformation. Using virtual alanine scanning site-directed mutagenesis, we identified critical residues influencing BER activity DSM 2243T. Mutations Y214A, K383A, K395A led to near-complete loss of enzymatic activity, highlighting their essential roles. Conversely, the E332Y, G393V, L515A variants demonstrated over 2-fold increase catalytic efficiency...
ABSTRACT Ferulic acid is a high‐value chemical synthesized in plants. The ferulic biosynthesis still affected by the insufficient methylation activity of caffeic O‐methyltransferase ( COMT ). In this study, we engineered from Arabidopsis thaliana to match acid, and mutant N129V‐H313A‐F174L showed 4.19‐fold enhanced catalytic efficiency for degrading acid. Then, constructed de novo synthesis pathway introducing tyrosine ammonia lyase Flavobacterium johnsoniae FjTAL ), 4‐hydroxyphenylacetate...
We examined the effects of a natural secondary bile acid, hyodeoxycholic acid (HDCA), on lipid metabolism and atherosclerosis in LDL receptor-null (LDLRKO) mice. Female LDLRKO mice were maintained Western diet for 8 wk then divided into 2 groups that received chow, or chow + 1.25% HDCA, diets 15 wk. observed fed HDCA leaner exhibited 37% (P<0.05) decrease fasting plasma glucose level. supplementation significantly decreased atherosclerotic lesion size at aortic root region, entire aorta,...
Maspin is an epithelial-specific tumor suppressor gene. Previous data suggest that maspin expression may redirect poorly differentiated cells to better phenotypes. Further, the first and only endogenous polypeptide inhibitor of histone deacetylase 1 (HDAC1) identified thus far. In current study, address what central program cell redifferentiation regulated by how microenvironments further define effects maspin, we conducted a systematic extensive comparison prostate grown in 2-dimensional...