A5085832630- Carbohydrate Chemistry and Synthesis
- Fungal Plant Pathogen Control
- Glycosylation and Glycoproteins Research
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Adipose Tissue and Metabolism
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Sirtuins and Resveratrol in Medicine
- Enzyme Production and Characterization
- Synthetic Organic Chemistry Methods
- Alcohol Consumption and Health Effects
- Photosynthetic Processes and Mechanisms
- Synthesis and biological activity
- Crystallography and molecular interactions
- Metabolism, Diabetes, and Cancer
- Enzyme Catalysis and Immobilization
- Lipid metabolism and biosynthesis
- Microbial Natural Products and Biosynthesis
- Mobile Agent-Based Network Management
- Gastroesophageal reflux and treatments
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
Guangdong Academy of Medical Sciences
2023-2025
Southern Medical University
2017-2025
Guangdong Provincial People's Hospital
2023-2025
Sun Yat-sen University
2025
The Seventh Affiliated Hospital of Sun Yat-sen University
2025
Zhujiang Hospital
2020-2024
Huazhong Agricultural University
2009-2024
Shanghai Zhangjiang Laboratory
2023-2024
Nanning Normal University
2024
Guangxi Normal University
2024
Alcoholic fatty liver is associated with inhibition of sirtuin 1 (SIRT1) and AMP-activated kinase (AMPK), two critical signaling molecules regulating the pathways hepatic lipid metabolism in animals. Resveratrol, a dietary polyphenol, has been identified as potent activator for both SIRT1 AMPK. In present study, we have carried out vivo animal experiments that test ability resveratrol to reverse inhibitory effects chronic ethanol feeding on SIRT1-AMPK system prevent development alcoholic...
Existing nanoparticle-mediated drug delivery systems for glioma systemic chemotherapy remain a great challenge due to poor efficiency resulting from the blood brain barrier/blood-(brain tumor) barrier (BBB/BBTB) and insufficient tumor penetration. Here, we demonstrate distinct design by patching doxorubicin-loaded heparin-based nanoparticles (DNs) onto surface of natural grapefruit extracellular vesicles (EVs), fabricate biomimetic EV-DNs, achieving efficient thus significantly enhancing...
Abstract Multidrug resistance remains a great challenge for cancer chemotherapy. Herein, biomimetic drug delivery system based on lemon‐derived extracellular vesicles (EVs) nanodrugs (marked with heparin‐cRGD‐EVs‐doxorubicin (HRED)) is demonstrated, achieving highly efficient overcoming multidrug resistance. The HRED fabricated by modifying functional heparin‐cRGD (HR) onto the surface of EVs and then loading doxorubicin (DOX). obtained enable to effectively enter DOX‐resistant cells...
Chronic ethanol feeding causes liver steatosis in animal models by upregulating the sterol regulatory element-binding protein 1 (SREBP-1), which subsequently increases synthesis of hepatic lipid. SREBP-1 activity is regulated reversible acetylation at specific lysine residues. The present study tests hypothesis that activation may be mediated mammalian sirtuin (SIRT1), a NAD(+)-dependent class III deacetylase. effects on SIRT1 were determined cultured rat hepatoma cells and livers...
The development of alcoholic fatty liver is associated with reduced adipocyte-derived adiponectin levels, decreased hepatic receptors, and deranged signaling in animals. Peroxisomal proliferator-activated receptor-gamma (PPAR-gamma) plays a key role the regulation adipose tissue. aim present study was to test ability rosiglitazone, known PPAR-gamma agonist, reverse inhibitory effects ethanol on expression its signaling, attenuate steatosis mice. Mice were fed modified Lieber-DeCarli...
Dysregulation of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis alcoholic liver injury. Sirtuin 1 (SIRT1) is an NAD(+)-dependent class III protein deacetylase that known to be involved regulating production including TNF-alpha. In present study, we examined role SIRT1 signaling TNF-alpha generation stimulated by either lipopolysaccharide (LPS), acetaldehyde (AcH), or acetate (two major metabolites ethanol) two cultured...
Abstract Lipin-1 is a protein that exhibits dual functions as phosphatidic acid phosphohydrolase enzyme in the triglyceride synthesis pathways and transcriptional coregulator. Our previous studies have shown ethanol causes fatty liver by activation of sterol regulatory element-binding 1 (SREBP-1) inhibition hepatic AMP-activated kinase (AMPK) mice. Here, we tested hypothesis AMPK-SREBP-1 signaling may be involved ethanol-mediated up-regulation lipin-1 gene expression. The effects on were...
Quality-of-life improvements have resulted in increasing attention being paid to research on antiaging and antioxidation. Polyphenols are natural antioxidants with excellent biological activities, such as antioxidation scavenging of free radicals antiviral activity. Abundant availability low toxicity polyphenols attracted the researchers. In this paper, antioxidant activities flavonoids, phenolic acids, stilbenes lignan analyzed, corresponding mechanisms investigated, effects systematically...
Abstract Microglia-mediated neuroinflammation plays a crucial role in the pathogenesis of Parkinson’s disease (PD). Triggering receptor expressed on myeloid cells 2 (TREM2) confers strong neuroprotective effects PD by regulating phenotype microglia. Recent studies suggest that TREM2 regulates high glucose-induced microglial inflammation through NLRP3 signaling pathway. This study aimed to investigate effect inflammasome activation and PD. Mice were injected with AAV-TREM2-shRNA into both...
Growing evidence has revealed the impacts of exposure to fine particulate matter (PM
Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, solution aid, would reduce productivity of enzyme. Esterification, solvent-free no-glycerol-release system for production, developed, two-step catalysis soybean oil, hydrolysis followed esterification, with Yarrowia lipolytica lipase is reported this paper.First, oil was hydrolyzed at 40°C 100 U broth...
Lipin-1 regulates lipid metabolism by way of its function as an enzyme in the triglyceride synthesis pathway and a transcriptional coregulatory protein is highly up-regulated alcoholic fatty liver disease. In present study, using liver-specific lipin-1-deficient (lipin-1LKO) mouse model, we aimed to investigate functional role lipin-1 development steatohepatitis explore underlying mechanisms. Alcoholic injury was achieved pair feeding wild-type lipin-1LKO mice with modified Lieber-DeCarli...
Alcohol consumption synergistically increases the risk and severity of liver damage in obese patients. To gain insight into cellular or molecular mechanisms underlying development fatty caused by ethanol-obesity synergism, we have carried out animal experiments that examine effects ethanol administration genetically mice. Lean wild-type (WT) (ob/ob) mice were subjected to feeding for 4 wk using a modified Lieber-DeCarli diet. After feeding, ob/ob displayed much more pronounced changes terms...
Ethanol-mediated injury, combined with gut-derived lipopolysaccharide (LPS), provokes generation of proinflammatory cytokines in Kupffer cells, causing hepatic inflammation. Among the mediators these effects, miR-217 aggravates ethanol-induced steatosis hepatocytes. However, role liver inflammation process is unknown. Here, we examined responses to ethanol, LPS, or a combination ethanol and LPS RAW 264.7 macrophages primary cells. In macrophages, substantially exacerbated LPS-mediated...
In order to develop potential glucosamine-6-phosphate synthase inhibitors and anti-fungal agents, twenty five oleanolic acid oxime esters were synthesized in an efficient way. The structures of the new compounds confirmed by MS, HRMS, 1H-NMR 13C-NMR. Preliminary studies based on means Elson-Morgan method indicated that many exhibited some inhibitory activity (GlmS), original fungicidal activities results showed good towards Sclerotinia sclerotiorum (Lib.) de Bary, Rhizoctonia solani Kuhn...
Thymoquinone, a natural occurring quinone and the main bioactive component of plant Nigella sativa, undergoes intracellular redox cycling re-oxidizes NADH to NAD+. TQ administration (20 mg/kg/bw/day) Diet-Induced Obesity (DIO) mice reduced their diabetic phenotype by decreasing fasting blood glucose insulin levels, improved tolerance sensitivity as evaluated oral tests (OGTT ITT). Furthermore, decreased serum cholesterol levels liver triglycerides, increased protein expression phosphorylated...
The aim of the present study is to examine effects dietary saturated fatty acids on liver adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2) in ethanol-administered animals ethanol-exposed cultured hepatic cells, explore underlying molecular mechanisms. mRNA protein levels AdipoR2 were selectively increased by chronic ethanol feeding mice consuming a diet high fat (HSF). Administration an HSF blocked hyperacetylation forkhead transcription factor (FoxO1), known target sirtuin (SIRT1), nuclear...
Our previous studies demonstrate enhanced neural protective effects of cord blood (CB) cells in comparison to stem from adult marrow. To determine further whether mesenchymal (MSCs) derived human umbilical (hUCB) possess optimal characteristics for therapy, we isolated populations plastic-adherent CB MSCs. These generated CD34-, CD45-, CD11b-, CD3-, CD19- culture and failed produce CFU-M, CFU-GEMM, or CFU-GM hematopoietic colonies methylcellulose. However, cultured MSCs possessed a...
Brassicaceae species employ both self-compatibility and self-incompatibility systems to regulate post-pollination events. Arabidopsis halleri is strictly self-incompatible, while the closely related thaliana has transitioned with loss of functional S-locus genes during evolution. The downstream signaling protein, ARC1, also required for response in some Brassica species, its gene deleted A. genome. In this study, we attempted reconstitute SCR-SRK-ARC1 pathway restore using from B. napus,...
Abstract Objective We aimed to identify key susceptibility gene targets in multiple datasets generated from postmortem brains and blood of Parkinson’s disease (PD) patients healthy controls (HC). Methods performed a multitiered analysis integrate the expression data using multiple-gene chips 244 human tissues. identified hub node genes highly PD-related consensus module by constructing protein–protein interaction (PPI) networks. Next, we validated top four interacting 238 subjects (90...