A5028207824- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant pathogens and resistance mechanisms
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Fermentation and Sensory Analysis
- CRISPR and Genetic Engineering
- Carbon Nanotubes in Composites
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Conducting polymers and applications
- Plant and animal studies
- Biochemical Analysis and Sensing Techniques
- Sperm and Testicular Function
Kunming Institute of Zoology
2025
Chinese Academy of Sciences
2009-2025
Huazhong Agricultural University
2016-2025
Army Medical University
2022-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2025
Gansu Provincial Maternal and Child Health Hospital
2025
Shanghai Ninth People's Hospital
2018-2024
Shanghai Jiao Tong University
2018-2024
Central South University
2020-2024
Xiangya Hospital Central South University
2022-2024
The analysis of 35,312 cannabis preparations confiscated in the USA over a period 18 years for delta-9-tetrahydrocannabinol (delta9-THC) and other major cannabinoids is reported. Samples were identified as cannabis, hashish, or hash oil. Cannabis samples further subdivided into marijuana (loose material, kilobricks buds), sinsemilla, Thai sticks ditchweed. data showed that more than 82% all category every year except 1980 (61%) 1981 (75%). potency (concentration delta9-THC) rose from less...
Flowering time adaptation is a major breeding goal in the allopolyploid species Brassica napus. To investigate genetic architecture of flowering time, genome-wide association study (GWAS) was conducted with diversity panel comprising 523 B. napus cultivars and inbred lines grown eight different environments. Genotyping performed 60K Illumina Infinium SNP array. A total 41 single-nucleotide polymorphisms (SNPs) distributed on 14 chromosomes were found to be associated 12 SNPs located...
S45A, a double recessive mutant at both the BnMs1 and BnMs2 loci in Brassica napus, produces no pollen mature anthers seeds by self-fertilization. The genes, which have redundant functions control of male fertility, are positioned on linkage groups N7 N16, respectively, located same locus Arabidopsis chromosome 1 based collinearity between Brassica. Complementation tests indicated that one candidate gene, BnCYP704B1, member cytochrome P450 family, can rescue sterility. Scanning electron...
Yellow seed is a desirable quality trait of the Brassica oilseed species. Previously, several coat color genes have been mapped in species, but molecular mechanism still unknown. In present investigation, map-based cloning method was used to identify gene, located on A9 B. rapa. Blast analysis with Arabidopsis genome showed that there were 22 this region including at4g09820 at4g10620. Functional complementation test exhibited phenotype reversion thaliana tt8-1 mutant and yellow-seeded plant....
A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by 60 K Infinium BeadChip Array. The contains 9164 SNP covering 1832.9 cM. 1232 bins account 7648 of markers. subset 2795 markers, with an average distance 0.66 cM between adjacent applied QTL mapping seed colour and cell wall fiber...
Drought is a major threat to plant growth and crop productivity. Reduced level of the gibberellin would result in increased drought tolerance, but underlying mechanism still unclear. In Brassica napus, there are four BnaRGA genes that code for DELLA proteins, negative regulators GA signaling. Among them, expression BnaA6.RGA was greatly induced by abscisic acid (ABA). Previously, we created gain-of-function mutant BnaA6.RGA, bnaa6.rga-D, loss-of-function quadruple mutant, bnarga CRISPR/Cas9,...
Brassica napus, an allotetraploid crop, is hypothesized to be a hybrid from unknown varieties of rapa and oleracea. Despite the economic importance B. much unresolved regarding its phylogenomic relationships, genetic structure, diversification. Here we conduct comprehensive study among diverse accessions 183 napus (including rapeseed, rutabaga, Siberian kale), 112 rapa, 62 oleracea wild relatives. Using RNA-seq accessions, define diversity sub-genome variance six clusters. Nuclear organellar...
Abstract The molecular mechanisms underlying anthocyanin-based flower coloration remain unknown in Brassica napus. To identify the key genes and metabolites associated with apricot pink colors, metabolome, BSA-seq, RNA-seq analyses were conducted on apricot-, pink-, yellow-, white-flowered F2B. Yellow carotenoids red anthocyanins abundant petals, while colorless accumulated petals. Most carotenoid not differentially regulated between yellow or white Three regulator genes, BnaMYBL2,...
Abstract Crossovers create genetic diversity and are required for equal chromosome segregation during meiosis. Crossover number distribution highly regulated by different mechanisms that not yet fully understood, including crossover interference. The axis is crucial formation. Here, we explore the function of protein ASYNAPSIS3. To this end, use allotetraploid species Brassica napus; due to its polyploid nature, system allows a fine-grained dissection dosage meiotic regulators. simultaneous...
Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect often associated with mitochondrial mutant.Approximately 10 chimeric genes been identified as being corresponding CMS systems the family Brassicaceae, but there little direct evidence these cause sterility.In study, novel gene (named orf288) was found to be located downstream of atp6 co-transcribed hau sterile line.Western blotting analysis...
Here, we describe the characteristics of a Brassica napus male sterile mutant 7365A with loss BnMs3 gene, which exhibits abnormal enlargement tapetal cells during meiosis. Later in development, absence gene results secretory function tapetum, as suggested by abortive callose dissolution and retarded degradation. The BnaC.Tic40 (equivalent to BnMs3) was isolated map-based cloning approach confirmed genetic complementation. Sequence analyses that originated from BolC.Tic40 on oleracea linkage...
Harvest index (HI), the ratio of grain yield to total biomass, is considered as a measure biological success in partitioning assimilated photosynthate harvestable product. While crop production can be dramatically improved by increasing HI, underlying molecular genetic mechanism HI rapeseed remains shown.In this study, we examined architecture using 35,791 high-throughput single nucleotide polymorphisms (SNPs) genotyped Illumina BrassicaSNP60 Bead Chip an association panel with 155...
Soil salinity is a serious threat to agriculture sustainability worldwide. Salt tolerance at the seedling stage crucial for plant establishment and high yield in saline soils; however, little information available on rapeseed (Brassica napus L.) salt tolerance. We evaluated different accessions conducted genome-wide association study (GWAS) identify tolerance-related quantitative trait loci (QTL). A natural population comprising 368 B. cultivars inbred lines was genotyped with Brassica 60K...
Abstract Luzhoulaojiao liquor is a type of Chinese that dates back hundreds years, but whose precise chemical composition remains unknown. This paper describes the screening and identification its compounds using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC × GC/TOF-MS). Samples were prepared by both liquid-liquid extraction solid-phase microextraction, which facilitated detection thousands in liquor, thus demonstrating superior performance proposed...
This study examined the effects of three different NaCl concentrations (60, 90, and 120 mM) on growth, physiological responses, steviol glycoside composition Stevia rebaudiana Bertoni for 4 weeks. The results showed that total dry weight decreased by 40% at mM but remained same 60 90 NaCl. As salt concentration increased, chlorophyll contents markedly 10-70%, whereas increments antioxidant enzyme activities were 1.0-1.6, 1.2-1.3, 2.0-4.0 times, respectively, superoxide dismutase, peroxidase,...
Summary WRKY transcription factors are known to participate in the defence responses of higher plants. However, little is about roles such proteins, especially regarding their functions resistance oilseed rape ( Brassica napus ) Sclerotinia sclerotiorum , a necrotrophic fungal pathogen that causes stem rot. In this study, we identified Bn 33 as S. ‐responsive gene positively regulates by enhancing expression genes involved camalexin synthesis and regulated salicylic acid SA jasmonic JA ). We...
Cytoplasmic male sterility (CMS) is not only important for exploiting heterosis in crop plants, but also as a model investigating nuclear-cytoplasmic interaction. CMS may be caused by mutations, rearrangement or recombination the mitochondrial genome. Understanding genome often first and key step unraveling molecular genetic basis of plants. Comparative analysis hau line its maintainer B. juneca (Brassica juncea) help show origin CMS-associated gene orf288. Through next-generation...
The Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rfp have been used in hybrid breeding Brassica napus, which has greatly improved the yield rapeseed. However, mechanism transition pol CMS remains to be determined. To investigate transcriptome during CMS, a near-isogenic line (NIL) was constructed. phenotypic features stage were confirmed by anatomical analysis. Subsequently, we compared genomic expression profiles fertile sterile young flower...
Stevia (Stevia rebaudiana) is an important medicinal plant that yields diterpenoid steviol glycosides (SGs). SGs are currently used in the preparation of medicines, food products and neutraceuticals because its sweetening property (zero calories about 300 times sweeter than sugar). Recently, some progress has been made understanding biosynthesis Stevia, but little known molecular mechanisms underlying this process. Additionally, genomics a non-model species, remains uncharacterized. The...