A5016334197- Protease and Inhibitor Mechanisms
- Bone health and treatments
- Prostate Cancer Treatment and Research
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Bladder and Urothelial Cancer Treatments
- Cancer, Lipids, and Metabolism
- Inflammatory mediators and NSAID effects
- Bone Metabolism and Diseases
- Ubiquitin and proteasome pathways
- Bone and Dental Protein Studies
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Cancer Diagnosis and Treatment
- Blood Coagulation and Thrombosis Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Cancer Treatment and Pharmacology
- Molecular Biology Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Cellular Mechanics and Interactions
- Renal Diseases and Glomerulopathies
Nova Southeastern University
2018-2023
Wayne State University
2007-2020
The Barbara Ann Karmanos Cancer Institute
2005-2020
HSBC Holdings
2020
Peking University
2020
Southeastern University
2018
University of Notre Dame
2005
Fundación para la Investigación, Docencia y Prevención del Cáncer
1994-2002
Consejo Nacional de Investigaciones Científicas y Técnicas
1987-2000
National University of Rosario
1998
Abstract BACKGROUND Hematopoietic cells home to bone by means of chemo‐attraction marrow chemokines, and interaction chemokines with their receptors leads the expression/activation adhesion molecules proteases. Recent evidence suggests that similar mechanisms may be active in cancer metastasis. Previously, we showed metalloproteases (MMPs), particular MMP‐9, play a role prostate (PC) expansion bone. METHODS We used variety methods including RT‐PCR, immunohistochemistry, ELISA, gelatin...
The importance of Discoidin Domain Receptor 1 (DDR1) in renal fibrosis has been shown via gene knockout and use antisense oligonucleotides; however, these techniques act a reduction DDR1 protein, while we prove the therapeutic potential inhibiting phosphorylation with small molecule. To date, efforts to generate selective small-molecule specifically modulate activity an vivo model have unsuccessful. We performed parallel DNA encoded library screens against DDR2, discovered chemical series...
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that play important roles in physiological and pathological conditions. Both gelatinases (MMP-2 -9) membrane-type 1 MMP (MMP-14) targets for inhibition, since their various diseases, including cancer, have been well established. We describe herein a set of mechanism-based inhibitors show high selectivity to MMP-14 (inhibitor 3) only MMP-2 (inhibitors 5 7). These molecules bind the active sites these enzymes, initiating slow...
Abstract Chemokines and their receptors function in migration homing of cells to target tissues. Recent evidence suggests that cancer use a chemokine receptor axis for metastasis formation at secondary sites. Previously, we showed binding the CXCL12 its CXCR4 mediated signaling events resulting matrix metalloproteinase-9 expression prostate bone metastasis. A variety methods, including lipid raft isolation, stable overexpression CXCR4, cellular adhesion, invasion assays, severe combined...
Journal Article Invasive and Metastatic Potential of a v-Ha-ras-Transformed Human Bronchial Epithelial Cell Line Get access R. Daniel Bonfil, Bonfil Search for other works by this author on: Oxford Academic PubMed Google Scholar Roger Reddel, Reddel Hitoshi Ura, Ura Reuven Reich, Reich R Fridman, Fridman Curtis C. Harris, Harris Andres J. P. Klein-Szanto JNCI: the National Cancer Institute, Volume 81, Issue 8, 19 April 1989, Pages 587–594, https://doi.org/10.1093/jnci/81.8.587 Published:...
Metastasis to the bone is a major clinical complication in patients with prostate cancer (PC). However, therapeutic options for treatment of PC metastasis are limited. Gelatinases members matrix metalloproteinase (MMP) family and have been shown play key role metastasis. Herein, we investigated effect SB-3CT, covalent mechanism-based MMP inhibitor high selectivity gelatinases, an experimental model metastases. Intraperitoneal (i.p.) SB-3CT (50 mg/kg) inhibited intraosseous growth human PC3...
Abstract The platelet-derived growth factor (PDGF) proteins are potent stimulators of cell proliferation/transformation and play a major role in cell-cell communication. For over two decades, PDGFs were thought to exist as three dimeric polypeptides (the homodimers AA BB the heterodimer AB). Recently, however, PDGF C D chains discovered BLAST search expressed sequence tag databases. CC DD dimers have unique two-domain structure with an NH2-terminal CUB (compliment subcomponents C1r/C1s,...
Abstract Bone is the key metastatic site for prostate cancer. Endothelin 1 (ET-1) produced abundantly by cancer cells binds to its receptor present on bone marrow stromal and favors osteoblastic response during metastases of This suggests that interrupting ET-1 interaction with endothelin A (ETA) could be useful inhibiting metastasis and, as such, may enhance therapeutic activity docetaxel (Taxotere), most commonly used drug treatment Therefore, goal our study was obtain preclinical data...
The tyrosine kinase receptor c-kit and its ligand stem cell factor (SCF) have not been explored in prostate cancer (PC) bone metastasis. Herein, we found that three human PC lines marrow stromal cells express a membrane-bound SCF isoform release soluble SCF. Bone revealed strong expression of c-kit, whereas showed very low levels the or did it all. Using an experimental model metastasis, intraosseous tumors formed by otherwise c-kit–negative PC3 strongly expressed as demonstrated using...
Abstract Membrane type 1 matrix metalloproteinase (MT1-MMP) plays an essential role in protease-mediated extracellular (ECM) degradation, but it also functions as a sheddase releasing non-ECM substrates such receptor activator of NF-κB ligand (RANKL), osteoclastogenic factor typically confined to the surface osteoblasts. We previously found high expression MT1-MMP skeletal metastasis prostate cancer patients, pattern similar RANKL expression. showed that overexpression cells increases tumor...
Triple-negative breast cancer (TNBC) studies have shown that neoadjuvant chemotherapy before surgery was effective in the minority of women, whereas majority who had residual tumor a relatively poor outcome. To identify mechanism by which cells survive chemotherapy, we initially conducted gene expression profiling using CRL2335 TNBC cell line derived from squamous carcinoma and after treatment with cisplatin plus TRAIL. We found significant increase FZD8, one Wnt receptors, its downstream...
Abstract At the cellular level, process of bone metastasis involves many steps. Circulating cancer cells enter marrow, proliferate, induce neovascularization, and ultimately expand into a clinically detectable, often symptomatic, metastatic deposit. Although initial establishment later expansion deposit in require tumor to possess invasive capability, exact proteases responsible for this phenotype are not well known. The objective our study was take an unbiased approach determine which were...
Maspin is an epithelial-specific tumor suppressor gene. Previous data suggest that maspin expression may redirect poorly differentiated cells to better phenotypes. Further, the first and only endogenous polypeptide inhibitor of histone deacetylase 1 (HDAC1) identified thus far. In current study, address what central program cell redifferentiation regulated by how microenvironments further define effects maspin, we conducted a systematic extensive comparison prostate grown in 2-dimensional...
Spindle cell carcinomas were identified using polyacrylamide gel electrophoresis and immunoblotting of proteins extracted from paraffin-embedded tissue sections. Immunohistochemistry rabbit monospecific antisera against the mouse 55 kd keratin polypeptide also these tumors. A group 53 SENCAR mice initiated with 7,12-dimethylbenz[a]anthracene (DMBA) promoted 12-O-tetradecanoylphorbol-13-acetate (TPA) yielded, after one year, four spindle (0.07/mouse), whereas another 31 treated a three-stage...