Plasma concentrations for several androgens and estrogens were determined in male domestic pigs from birth to eight months of age. Samples (N = 6) blood collected Yorkshire males at weekly intervals four weeks, thereafter monthly months. Radioimmunoassays done without extraction plasma dehydroepiandrosterone sulphate, androstenedione estrone sulphate. Other steroids measured after solid-phase extraction, separate elution unconjugated conjugated fractions, solvolysis sulpho-conjugated...

Purified Leydig cells were obtained from testes of mature male pigs by collagenase treatment and mechanical dispersion, followed Percoll (0–90%) density gradient centrifugation. The recovered at 40–45% applied to a second 15 ml (10–60%) yield three bands, one major two lesser in numbers cells. Incubations then made with 0.25–1.0 × 106 34°C for 3 h 95% O2: 5% CO2, or without human chorionic gonadotrophin (hCG) added the medium. Steroid concentration was determined radioimmunoassays. steroids...

SUMMARY Copulation with an oestrous sow or exposure to aggressive boar resulted in a sharp rise the concentration of corticosteroids plasma boars, and subsequently, immediate increase testosterone. Placing test close proximity for period 10 h caused three peaks plasma, each which preceded peak testosterone concentration. An inhibitor 11 β-hydroxylation, metyrapone, was administered intravenously limit corticosteroid production. One injection 5 mg metyrapone/kg body weight injections 4 did...

Summary. The effects of oestrogens on the activity accessory sex glands were studied in castrated boars receiving testosterone continuously. Diethylstilboestrol (DES), 17β-oestradiol (E2) and oestrone (E1) administered successively for 6-week periods which alternated with treatments alone. Semen was collected twice each week measurements made total, strained gel volumes. Citric acid fructose contents seminal plasma also determined. Highly significant (P<0·01) differences between observed all...

Abstract An extraction and assay procedure to measure fecal estradiol‐17β progestin concentrations in several cat species was developed validated for use noninvasive monitoring of ovarian function. Fecal samples were collected over a range 3–20 months from female tigers (three), lions snow leopards cheetahs (two), caracals domestic cats (five). Samples extracted with 90% methanol, lipids removed petroleum ether, the estradiol progestins methanol measured by radioimmunoassay (RIA). High...

Injection of a 'rapid-acting' preparation porcine adrenocorticotrophic hormone (ACTH) into three boars resulted in rapid rise plasma testosterone levels which accompanied the expected corticosteroids. Urinary dehydroepiandrosterone (DHA) were measured one boar and found to be raised also. The results suggest that effect involved enhaced testicular steroid activity was related dosage ACTH employed. This action is thought mediated through adrenal cortex since injection cortisol elecited...

The content and concentration of testosterone in fetal gonads domestic pigs was investigated for the early stages pregnancy. Measurements were made with a competitive-protein binding radioassay after column chromatography extracts. Gonads obtained from 283 male fetuses ranging size 2.0 to 12.0 cm crown-rump (C-R) length. Testosterone present measurable amounts smallest specimens reached peak >4.0 ng per pair near 4.0 C-R stage. A decrease testicular values then noted until 5.1–5.3 length...

Breeding soundness evaluation (BSE) is the primary assessment for determining reproductive potential of male animals. This method, however, cannot be used to evaluate semen frequently or predict future quality. Computerized analysis ultrasonographic images provides information on histophysiological changes in organs. We hypothesized that: (i) parameters would correlate with characteristics distal region (cauda) epididymis and (ii) testicular ultrasound and/or circulating testosterone...

A method is described for the extraction and purification of estrogen sulfates from testicular tissues. By use this estrone 3-sulfate estradiol-17β were isolated stallion testes identified by infrared spectroscopy. Further characterization was effected estrone-4- 14 C estrone-6,7- 3 H as standards chromatographic comparison. Preliminary results indicated that amounts estrogens present greatly in excess free steroid. Estradiol-17α could not be detected.

Summary. The daily urinary excretion of oestrogens was measured by a chemical method, at weekly intervals through the greater part gestation in two sows and during last 6 weeks four other sows. Oestrone appeared to be principal component pregnant sows' urine. After an early peak 4th week from conception, level oestrone remained low until about 10th then rose steadily high levels term. Complete continuous collection urine, uncontaminated with faeces, made for several days before parturition...

Urinary levels of estriol, estrone and estradiol-17β (estradiol) increase during the last 20 weeks pregnancy in a qualitatively similar fashion both normal diabetic states. The relative amounts these estrogens found urine suggest that they arise from same source groups according to transformation, estradiol⇌estrone→estriol. It also appears likely there is an additional estriol two groups, particularly late pregnancy. There tendency for be lower than nondiabetic Considerable variability 3 one...

SUMMARY Levels of interstitial cell-stimulating hormone (ICSH) activity in plasma estimated by the rat ovarian cholesterol depletion method and urinary gonadal steroid excretion were studied boars under various experimental conditions. Injections adrenocorticotrophic (ACTH), hydrocortisone prednisolone trimethylacetate found to lower level ICSH dehydroepiandrosterone (DHA) oestrogens. This action ACTH is thought be mediated through adrenal cortex since did not reduce DHA or adrenalectomized...

Because estradiol (E2) production by the early equine conceptus is considered crucial to establishment of pregnancy, amounts E2, estrone (E1), and their sulfates (E2S, E1S) were measured RIA in yolk-sac fluid 63 conceptuses collected transcervical lavage over period 11–26 days after ovulation. Amounts increased significantly with age conceptus, especially for E1S. Then, metabolism which may be highly relevant its action, was examined endometrium when ceases migrate within uterus. Eleven...

ABSTRACT The urinary excretion of dehydro epi androsterone (DHA), oestrone (OE 1 ) and 17β-oestradiol 2 has been estimated during the administration a number hormones to an intact mature male pig. Daily intramuscular injections corticotrophin (300 IU) or cortisone acetate (500 mg) resulted in decrease levels OE , DHA. Following daily injection human chorionic gonadotrophin (2500 all three steroids increased greatly. oral 30 mg Norlutin (17α-ethynyl-19-nortestosterone acetate) appeared be...

The effect of intracarotid perfusion 40 mg cortisol for 1 h on the hormonal response to three different doses an intramuscular injection synthetic gonadotrophin releasing hormone (GnRH) was compared that GnRH injected during with 0·9% (w/v) NaCl solution in five boars. increase production LH, above basal values, 0·25 μg midway through only slightly greater ( P &gt; 0·05) boars receiving when same received saline. When 0·5 perfusion, a significantly &lt;0·05) LH levels occurred administration...

The production and secretion of testosterone by fetal pig testes in vitro was investigated at the stage sexual differentiation. Single gonads from 76 pigs between 2.0 5.2 cm crown-rump (C-R) length were cultivated for 36 h on synthetic TC Medium 199. Steroids extracted media (n = 72) explants 46) with methylene chloride. Contralateral 58) without cultivation. Testosterone isolated partition chromatography Celite measured a competitive protein-binding radioassay. earliest development which...

Early studies suggested the placenta as source of oestrogens in pregnant mare (Catchpole & Cole, 1934).Ainsworth Ryan (1966), however, were unable to demonstrate oestrogen formation from C21-steroids by placental preparations mare, although C19-steroids aromatized oestrogens.A feto-placental unit for biosynthesis is these findings and inactive appearance maternal ovaries mid-pregnancy, when production increases rapidly very high levels 1934).At this time fetal gonads show a remarkable...