A5061770335- Metabolomics and Mass Spectrometry Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic function and diabetes
- CAR-T cell therapy research
- Analytical Chemistry and Chromatography
- Cancer, Hypoxia, and Metabolism
- Redox biology and oxidative stress
- Autophagy in Disease and Therapy
- Mesenchymal stem cell research
- Spectroscopy and Chemometric Analyses
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Glioma Diagnosis and Treatment
- Molecular spectroscopy and chirality
- Photosynthetic Processes and Mechanisms
- Vascular Tumors and Angiosarcomas
- Bioinformatics and Genomic Networks
- Biopolymer Synthesis and Applications
- Amino Acid Enzymes and Metabolism
- Electron Spin Resonance Studies
- vaccines and immunoinformatics approaches
- Cell Image Analysis Techniques
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Endoplasmic Reticulum Stress and Disease
Greenwood Genetic Center
2024
University of Georgia
2019-2023
Istituto di Sociologia Internazionale di Gorizia
1989
Abstract Mesenchymal stromal cells (MSCs) have shown promise in regenerative medicine applications due part to their ability modulate immune cells. However, MSCs demonstrate significant functional heterogeneity terms of immunomodulatory function because differences MSC donor/tissue source, as well non-standardized manufacturing approaches. As metabolism plays a critical role expand therapeutic numbers ex vivo, we comprehensively profiled intracellular and extracellular metabolites throughout...
Evolutionarily conserved cysteine residues mediate the sensitivity of metabolic kinase FN3K to shifts in redox status.
Abstract Mono- and bi-allelic variants in ALDH18A1 cause a spectrum of human disorders associated with cutaneous neurological findings that overlap both cutis laxa spastic paraplegia. encodes the bifunctional enzyme pyrroline-5-carboxylate synthetase (P5CS) plays role de novo biosynthesis proline ornithine. Here we characterize previously unreported homozygous variant (p.Thr331Pro) four affected probands from two unrelated families, demonstrate broad-based alterations amino acid antioxidant...
Large-scale, reproducible manufacturing of therapeutic cells with consistently high quality is vital for translation to clinically effective and widely accessible cell therapies. However, the biological logistical complexity a living product, including challenges associated their inherent variability uncertainties process parameters, currently make it difficult achieve predictable cell-product quality. Using degradable microscaffold-based T-cell process, we developed an artificial...
Chimeric antigen receptor (CAR) T cells have demonstrated remarkable efficacy against hematological malignancies; however, they not experienced the same success solid tumors such as glioblastoma (GBM). There is a growing need for high-throughput functional screening platforms to measure CAR T-cell potency tumor cells.
Abstract Aberrant regulation of metabolic kinases by altered redox homeostasis is a major contributing factor in aging and disease such as diabetes. However, the biochemical mechanisms which are regulated under oxidative stress poorly understood. In this study, we demonstrate that catalytic activity conserved family Fructosamine-3-kinases (FN3Ks), evolutionarily related to eukaryotic protein (ePKs), redox-active cysteines kinase domain. By solving crystal structure FN3K homolog from...
Natural products and metabolomics are intrinsically linked by the efforts of analyzing complex mixtures for compound annotation. Although most studies that aims identification in use MS as main analysis technique, NMR has complementary advances worth exploring enhanced structure confidence. This review intends to showcase a portfolio tools available using NMR. COLMAR, SMART-NMR, MADByTE, NMRfilter presented examples collected with real samples from perspective natural chemist.
Abstract Large-scale, reproducible manufacturing of therapeutic cells with consistently high quality is vital for translation to clinically effective and widely accessible cell therapies. However, the biological logistical complexity a living product, including challenges associated their inherent variability uncertainties process parameters, currently make it difficult achieve predictable cell-product quality. Using degradable microscaffold-based T as an example, we developed Artificial...
Abstract Mesenchymal stromal cells (MSCs) have shown promise in regenerative medicine applications due part to their ability modulate immune cells. However, MSCs demonstrate significant functional heterogeneity terms of immunomodulatory function because differences MSC donor/tissue source, as well non-standardized manufacturing approaches. As metabolism plays a critical role expand therapeutic numbers ex vivo , we comprehensively profiled intracellular and extracellular metabolites...
Carcinomas have two broadly distinct modes of invasion: collective and individual. Collective invasion is characterized as groups cells that migrate while retaining cell-cell contacts. Individual occurs through mechanisms such the acquiring mesenchymal-like features by cancer cells. While these different been well studied at genomic transcriptional levels, their metabolic alterations remain much less understood. To address this deficiency, we performed untargeted NMR metabolomics, in...
Abstract Carcinomas have two broadly distinct modes of invasion: collective and individual. Collective invasion is characterized as groups cells that migrate while retaining cell-cell contacts. Individual occurs through mechanisms such the acquiring mesenchymal-like features by cancer cells. While these different been well studied at genomic transcriptional levels, their metabolic alterations remain much less understood. To address this deficiency, we performed untargeted NMR metabolomics,...
Abstract Glioblastoma (GBM), like most cancers, undergo metabolic alterations to primarily utilize aerobic glycolysis in the hypoxic tumor microenvironment (TME). Similarly, activated T cells switch upon antigen recognition cope with proliferation needs but are not equally equipped survive TME. Metabolic reprogramming within GBM TME contributes therapeutic resistance and progression, effects of on cell survival efficacy have been fully elucidated. We hypothesized that hypoxia GBM/T-cell...