A5000730641- Cellular Mechanics and Interactions
- Enzyme Production and Characterization
- PI3K/AKT/mTOR signaling in cancer
- Enzyme Catalysis and Immobilization
- Microtubule and mitosis dynamics
- Fungal Biology and Applications
- Autophagy in Disease and Therapy
- Inflammasome and immune disorders
- Enzyme-mediated dye degradation
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Redox biology and oxidative stress
- Research on Leishmaniasis Studies
- Protein Kinase Regulation and GTPase Signaling
- Phagocytosis and Immune Regulation
- Signaling Pathways in Disease
- Slime Mold and Myxomycetes Research
- Erythrocyte Function and Pathophysiology
- Biofuel production and bioconversion
- Cellular transport and secretion
- Biocrusts and Microbial Ecology
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Fungal and yeast genetics research
- Planarian Biology and Electrostimulation
National Institute of Diabetes and Digestive and Kidney Diseases
2018-2025
National Institutes of Health
2018-2025
Indian Institute of Technology Madras
2012-2024
Institute of Life Sciences
2021-2022
Kinases mTORC1 and AMPK act as energy sensors, controlling nutrient responses cellular growth. Changes in levels affect diverse transcriptional networks, making it challenging to identify downstream paths that regulate growth or a switch development via variation. The life cycle of Dictyostelium presents an excellent model study the signaling function for development. grow single cells nutrient-rich media, but, upon withdrawal, ceases enter program multi-cell While nearly half genome shows...
Abstract The NOD1/2‐RIPK2 is a key cytosolic signaling complex that activates NF‐κB pro‐inflammatory response against invading pathogens. However, uncontrolled can cause tissue damage leading to chronic diseases. mechanisms by which the NODs‐RIPK2‐NF‐κB innate immune axis activated and resolved remain poorly understood. Here, we demonstrate bacterial infection induces formation of endogenous RIPK2 oligomers (RIPosomes) are self‐assembling entities coat bacteria induce response. Next, show...
Self-organized and excitable signaling activities play important roles in a wide range of cellular functions eukaryotic prokaryotic cells. Cells require networks to communicate amongst themselves, but also for response environmental cues. Such signals involve complex spatial temporal loops that may propagate as oscillations or waves. When Dictyostelium become starved nutrients, cells within localized space begin secrete cAMP. Starved chemotactic cAMP outwardly moving waves oscillate at ∼6...
The growing demand in industrial and biotechnological settings for more efficient enzymes with enhanced biochemical features, particularly thermostability thermotolerance, necessitates a timely response. Renowned their versatility, thermostable offer significant promise across range of applications, including agricultural, medicinal, domains. This comprehensive review summarizes the structural attributes, catalytic mechanisms, connection between configuration functional activity two major...
Abstract Background Multicellularity in cellular slime molds is achieved by aggregation of several hundreds to thousands cells. In the model mold Dictyostelium discoideum , adenosine known increase aggregate size and its antagonist caffeine reduces size. However, it not clear if actions are evolutionarily conserved among other use structurally unrelated chemoattractants. We have examined how factors affecting modulated caffeine. Result Adenosine induced formation large small aggregates...
The growing demand in industrial and biotechnological settings for more efficient enzymes with enhanced biochemical features, particularly thermostability thermotolerance, necessitates a timely response. Renowned their versatility, thermostable enzymes, offer significant promise across range of applications, including agricultural, medicinal, domains. This comprehensive review summarizes the structural attributes, catalytic mechanisms, relationships between structure function two major...
Abstract Background The multicellular slug in Dictyostelium has a single tip that acts as an organising centre patterning the rest of slug. High adenosine levels at are believed to be responsible for this dominance and antagonist, caffeine overrides promoting multiple formation. Results Caffeine induced effect is conserved all Dictyostelids tested. Two key components cAMP relay namely, phosphodiesterase (Pde4) adenyl cyclase-A (AcaA) get reduced during secondary formation discoideum ....
The mechanistic Target of Rapamycin Complex 1 (mTORC1) regulates ribosome biogenesis and the transcription ribosomal genes. Its inactivation impacts protein synthesis necessary for transport cell adhesion molecule. In Dictyostelium discoideum, activity 2 (mTORC2) is crucial cAMP relay, processes that depend on heterotrimeric G-proteins Ras GTPases. Mutants lacking rip3 or lst-8 genes exhibit impaired adenyl cyclase activation signaling, resulting in deficient expression CsaA. Despite this,...
ABSTRACTCaffeine is known to impair both growth and development in the model social amoeba Dictyostelium discoideum. However, little about transcriptional regulation response caffeine treatment Dictyostelium. In yeast cells, mimics effect of rapamycin inhibiting mTORC1 activity. Here, we investigated whether changes presence or are mediated by and/or mTORC2. Results: Treatment wild type cells with resulted severely impaired formation small aggregates comparison untreated cells....
Nutrient uptake via phagocytosis and pinocytosis is essential for the survival growth of Dictyostelium discoideum, with mTOR signaling pathway, comprising mTORC1 mTORC2 complexes, playing a pivotal regulatory role. This study investigated how caffeine adenosine influence these processes in wild-type (KAX3) mTORC2-deficient (lst8- rip3-) cells. Caffeine enhanced rates while inhibiting fluid-phase KAX3 cells, implicating as target. Similarly, lst8- rip3- mutants exhibited increased...
Abstract Background Cellular functions can be regulated by cell-cell interactions that are influenced extra-cellular, density-dependent signaling factors. Dictyostelium grow as individual cells in nutrient-rich sources, but, nutrients become depleted, they initiate a multi-cell developmental program is dependent upon cell-density threshold. We hypothesized novel secreted proteins may serve density-sensing factors to promote fate decisions at specific threshold, and use the identification of...
The TOR kinase functions in two protein complexes, mTORC1 and mTORC2. is a key regulator for cell growth, sensing signals from nutrients energy levels. While activity inhibited by nutrient removal, it also inactivated the immunosuppressive drug rapamycin. In contrast, mTORC2 does not regulate insensitive to direct action of rapamycin, but crucial developmental pathways. mTOR complexes are critical life cycle Dictyostelium where transition growth‐to‐development initiated upon depletion. Thus,...