A5005286577- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Advanced Electron Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Metal and Thin Film Mechanics
- Nitric Oxide and Endothelin Effects
- Anesthesia and Neurotoxicity Research
- Glycosylation and Glycoproteins Research
- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Semiconductor materials and devices
- RNA Interference and Gene Delivery
- Anesthesia and Sedative Agents
- Surface Roughness and Optical Measurements
- Plasma Diagnostics and Applications
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Genetic Neurodegenerative Diseases
- Carbohydrate Chemistry and Synthesis
- Olfactory and Sensory Function Studies
- Optical Coatings and Gratings
Friedrich Schiller University Jena
2015-2025
Helmholtz Institute Jena
2025
Maksymilian Pluta Institute of Applied Optics
2024
University Hospital Leipzig
2022-2024
Leibniz Institute of Photonic Technology
2024
Max Planck Institute of Molecular Cell Biology and Genetics
2018-2021
Fraunhofer Institute for Applied Optics and Precision Engineering
2014-2019
University of Würzburg
2013-2017
Leiden University
2016
Albert Einstein College of Medicine
2016
Delivery of exogenous mRNA using lipid nanoparticles (LNPs) is a promising strategy for therapeutics. However, bottleneck remains in the poor understanding parameters that correlate with endosomal escape versus cytotoxicity. To address this problem, we compared distribution six LNP-mRNA formulations diverse chemical composition and efficacy, similar to those used mRNA-based vaccines, primary human adipocytes, fibroblasts, HeLa cells. Surprisingly, found total uptake not sufficient predictor...
Far-red cyanine fluorophores find extensive use in modern microscopy despite modest quantum yields. To improve the photon output of these molecules, we report a synthetic strategy that blocks major deactivation pathway: excited-state trans-to-cis polyene rotation. In key transformation, protected dialdehyde precursor undergoes cascade reaction to install requisite tetracyclic ring system. The resulting molecules exhibit characteristic features conformational restraint, including improved...
Here, we combine super-resolution fluorescence localization microscopy with scanning electron to map the position of proteins nuclear pore complexes in isolated Xenopus laevis oocyte envelopes molecular resolution both imaging modes. We use periodic structure complex superimpose direct stochastic optical reconstruction images a precision <20 nm on micrographs. The correlative demonstrate quantitative labeling and by standard immunocytochemistry primary secondary antibodies reveal that is...
Significance Synaptonemal complexes (SCs) are meiosis-specific, 200-nm-wide, ladder-like structures that essential for synapsis, recombination, and segregation of homologous chromosomes. Despite its importance the spatial organization individual components during meiosis, molecular architecture SC is still unknown. We used super-resolution imaging by direct stochastic optical reconstruction microscopy ( d STORM), which provides subdiffraction resolution structural investigation SC. The...
Abstract Tactile sensation and vision are often both utilized for the exploration of objects that within reach though it is not known whether or how these two distinct sensory systems combine such information. Here in mice, we used a combination stereo photogrammetry 3D reconstruction whisker array, brain-wide anatomical tracing functional connectivity analysis to explore possibility tacto-visual convergence space circuitry primary visual cortex (VISp). Strikingly, find stimulation...
Abstract Much of the physiology cells is controlled by spatial organization plasma membrane and glycosylation patterns its components, however, studying distribution, size, composition these components remains challenging. A bioorthogonal chemical reporter strategy was used for efficient specific labeling membrane‐associated glycoconjugates with modified monosaccharide precursors organic fluorophores. Super‐resolution fluorescence imaging to visualize glycans single‐molecule sensitivity. Our...
Abstract Many cellular organelles, including endosomes, show compartmentalization into distinct functional domains, which, however, cannot be resolved by diffraction‐limited light microscopy. Single molecule localization microscopy (SMLM) offers nanoscale resolution but data interpretation is often inconclusive when the ultrastructural context missing. Correlative electron (CLEM) combining SMLM with (EM) enables correlation of subdomains organelles in relation to their underlying...
1. Electrical and contractile properties of resealed fibre segments were investigated by a variety in vitro techniques. The preparations removed from skeletal muscles normal subjects eight patients with myotonic dystrophy. 2. Several hours after removal, those whom myotonia was the primary symptom had resting membrane potentials approximately ‐80 mV. In contrast, obtained muscle dystrophy more expressed depolarized (‐60 to ‐70 mV). 3. Contractions induced which characterized slowed...
The development of small molecule activity-based probes (ABPs) is an evolving and powerful area chemistry. There a major need for synthetically accessible specific ABPs to advance our understanding enzymes in health disease. α-Glucosidases are involved diverse physiological processes including carbohydrate assimilation the gastrointestinal tract, glycoprotein processing endoplasmic reticulum (ER), intralysosomal glycogen catabolism. Inherited deficiency lysosomal acid α-glucosidase (GAA)...
Abstract Polyoxazolines have long been considered as promising alternatives to poly(ethylene glycol) (PEG) due their comparable properties, in particular regarding stealth effect toward the immune system. Lipid nanoparticles (LNPs), utilized, e.g., COVID‐19 vaccines, contain PEG‐lipids. However, are required because of “PEG dilemma” recognized by an increase anti‐PEG antibodies human population. In this study, poly(2‐ethyl‐2‐oxazoline) (PEtOx)‐based lipids with different degrees...
Single channel recordings have shown that ketamine (Ket) decreases the open time of nicotinic acetylcholine receptor (nAChR).The present experiments on simultaneous openings nAChRs mouse myotubes investigate interaction Ket with as well closed state channels. The patch-clamp technique was used to record currents activated by 10-4 M (ACh) in outside-out mode. ACh together increasing concentrations applied a piezo-driven system. In second protocol, patches were preexposed before activation...
Background This study was performed to elucidate and compare the effects of sevoflurane isoflurane on nicotinic acetylcholine receptor mouse myotubes. The experiments were done with special reference anesthetic concentrations considerably less than those used for clinical anesthesia. Methods patch-clamp technique record acetylcholine-activated currents from embryonic type in outside-out mode. A piezo-driven liquid filament switch ultrafast application alone or combination sevoflurane. In...
Lipid rafts are membrane microdomains specialized in the regulation of numerous cellular processes related to organization, as diverse signal transduction, protein sorting, trafficking or pathogen invasion. It has been proposed that this functional diversity would require a heterogeneous population raft domains with varying compositions. However, mechanism for such diversification is not known. We recently discovered bacterial membranes organize their transduction pathways (FMMs)...
Alterations of 5‐hydroxytryptaminergic mechanisms are thought to play a special role in the pathogenesis depression and antidepressant treatments assumed restore these changes. We have used one most reliable models depression, olfactory bulbectomized rat study long term consequences this manipulation subchronic imipramine treatment on two parameters presynapses, 5‐hydroxytryptamine (5‐HT) transporter density tryptophan hydroxylase apoenzyme concentration, frontal cortex as well active...
Whole-cell and single-channel voltage-clamp techniques were used to identify characterize the ionic currents of insect olfactory receptor neurons (ORNs) in vitro. The cells isolated from antennae male Manduca sexta pupae at stages 3–5 adult development maintained primary cell culture. After 2–3 weeks vitro, presumptive ORNs had resting potentials -62 +/- 12 mV (n = 18) expressed least 1 type Na+ channel 3 types K+ channels. currents, recorded whole-cell mode, reversibly blocked by 0.1 microM...
Lumen morphogenesis results from the interplay between molecular pathways and mechanical forces. In several organs, epithelial cells share their apical surfaces to form a tubular lumen. liver, however, hepatocytes surface only adjacent narrow lumina that grow anisotropically, generating 3D network of bile canaliculi (BC). Here, by studying lumenogenesis in differentiating mouse hepatoblasts vitro, we discovered assemble pattern specific extensions membrane traversing lumen ensuring its...