Temporal genomic profiling and whole-cell proteomic analyses were performed to characterize the dynamic molecular response of metal-reducing bacterium Shewanella oneidensis MR-1 an acute chromate shock. The complex dynamics cellular processes demand integration methodologies that describe biological systems at levels regulation, gene protein expression, metabolite production. Genomic microarray analysis transcriptome midexponential phase cells subjected 1 mm potassium (K2CrO4) exposure time...

The biological impact of 24-h ("chronic") chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration 0.3 mM in complex growth medium were compared to untreated control cells grown the absence chromate. At time point at which harvested for transcriptome proteome analyses, no residual Cr(VI)...

Shotgun proteomics experiments require the collection of thousands tandem mass spectra; these sets data will continue to grow as new instruments become available that can scan at even higher rates. Such contain substantial amounts redundancy with spectra from a particular peptide being acquired many times during single LC-MS/MS experiment. In this article, we present MS2Grouper, an algorithm detects spectral duplication, assesses groups related spectra, and replaces synthetic representative...

Proteome alterations in the metal-reducing bacterium Shewanella oneidensis MR-1 response to different acute dose challenges (0.3, 0.5, or 1 mM) of toxic metal chromate [Cr(VI)] were characterized with multidimensional HPLC−MS/MS. measurements performed and compared on both quadrupole ion traps as well linear trapping mass spectrometers. We have found that implementation liquid chromatography on-line rapid scanning, high throughput platform resulted a dramatic increase number measured...

Algorithmic search engines bridge the gap between large tandem mass spectrometry data sets and identification of proteins associated with biological samples. Improvements in these tools can greatly enhance discovery. We present a new scoring scheme for comparing spectra protein sequence database. The MASPIC (Multinomial Algorithm Spectral Profile-based Intensity Comparison) scorer converts an experimental spectrum into m/z profile probability then scores peak lists from potential candidate...

High-performance (HP) coaching has been described as “a complex, social, and dynamic activity that is not easily represented a set of tangible predictable processes.” Coaches are only responsible for extensive planning, monitoring, leadership in complex environment but also have responsibility supporting athlete development safeguarding their athletes’ overall health, well-being, psychological physiological safety. However, HP often considered an unsustainable profession, due to the levels...

Abstract A CGE method for monitoring the disulfide isomer distribution characteristic of IgG2 MAbs is presented. Disulfide heterogeneity has been studied using various chromatographic and electrophoretic methods. Although operates a different selectivity mechanism from that sorption techniques, similar trends are present in data, which allow to be used as complementary studying distribution. This article focuses on optimization capillary‐based gel electrophoresis can support antibody...

Many methods of microbial proteome characterizations require large quantities cellular biomass (>1−2 g) for sample preparation and protein identification. Our experimental approach differs from traditional techniques by providing the ability to identify proteomic state a microbe few milligrams starting material. The small-scale, guanidine lysis method minimizes loss achieving digestion in single-tube experiment. For this approach, freshwater Shewanella oneidensis MR-1 purple non-sulfur...

The first synthesis of the repeating unit S. aureus capsular polysaccharide type 8 is described. an unusual trisaccharide sequence three uncommon sugars, all connected via 1,2-cis linkages. synthetic was equipped with capping methyl groups at points propagation sequence.

There appears to be a relationship between coaching efficacy and athlete satisfaction, confidence, performance, as well coach behavior. However, little research exists, specifically within high school setting, examining the coaches’ perceptions of those stakeholders (parents athletes). The purpose this was examine stakeholders’ views efficacy. A sample 516 coaches, 115 athletes, 103 parents were recruited. Coaches have significantly higher levels on all four domains than ratings from...

The purpose of the present study was to examine learning experiences a high performance, head baseball coach within community. We drew upon barriers and enablers community structure current study's intervention. Our comprised one collegiate summer league three educators (i.e. facilitators). Data were collected via eight weekly meetings recorded transcribed), coach's daily journal post-learning meeting journal, memory his coaching experiences. findings, which suggest potential framework for...

The chemical synthesis of the repeating unit S. aureus capsular polysaccharide type 5 equipped with capping methyl groups at points propagation sequence is described. This model compound was designed to study activation full length for conjugation a carrier protein.

Predicted orphan response regulators encoded in the Shewanella oneidensis MR-1 genome are poorly understood from a cellular function perspective. Our previous transcriptomic and proteomic analyses demonstrated that an annotated DNA-binding regulator, SO2426, was significantly up-regulated wild-type S. cells at both mRNA protein levels to acute chromate [Cr(VI)] challenge, suggesting potential regulatory role for this metal stress pathways. To investigate impact of SO2426 activity on...

Academic coach education programmes in the USA have been developed to offer a comprehensive approach preparation and development. However, little is known about how these academic prepare student coaches for coaching profession, particularly with regard practicum or internship experiences. This study surveyed programme coordinators university setting explored current programmatic methods of implementing internship. The was found vary widely structure expectations, leading three main...

As the sport coaching profession continues to grow, there is a need reflect upon and revise knowledge competencies coaches should possess support quality experiences. The purpose of this paper document revision process National Standards for Sport Coaches (NSSC) which were established outline professional standards in United States America (USA). 3-year involved two separate task forces organized by SHAPE several public reviews. final aligns NSSC with frameworks documents seven core...

Abstract The current study is part of a community engaged planning phase and aimed to identify perceptions related the prevalence violent crime, crime contributors, relationship between law enforcement, potential interventions solutions. In March 2021, semistructured interviews were conducted with individuals from five groups who resided or worked in Bessemer, Alabama: (1) Law Enforcement, (2) Residents, (3) Civic Leaders, (4) Community (5) Victims Crime. Interviews lasted approximately 60...

Commencing in 2014, Quadrant Energy and partners have undertaken an active exploration program the Bedout Sub-basin with a 100% success rate, discovering four hydrocarbon accumulations wells. The primary target basin, Middle Triassic Lower Keraudren Formation, encompasses reservoirs, source rocks seals that trapped hydrocarbons self-contained petroleum system. This system is older than traditional plays on North-West Shelf before recent activity was very poorly understood easily overlooked....