Desiccation tolerance has emerged as a potential strategy for crop improvement. However, the application of desiccation in crops, particularly rice, been challenging and limited. Studies angiosperms suggest that vegetative tissues shares common set genes, developed convergently by modifying shared pathways associated with seed desiccation. The drought-tolerant Oryza sativa ssp. indica cv MR303 demonstrated capability to withstand state short period time. Using this variety, we aim...

The harmful effects of chemical herbicides on the environment and human health have increased interest in natural bioherbicides. This study analyzed global trends bioherbicide research for controlling weeds from 2002 to 2022 using bibliometric analysis. analysis presented trend based relevant topics, an annual publication 20 years, co-occurrence countries institutions, productive journals, frequent keywords used. Scopus database resulted 1472 articles tools, R-4.2.2 software with...

Problem statement: Comparison and validation analysis of the conventional power-adjusted Microwave-Assisted (MA) techniques TMS derivatization using Gas Chromatography-Mass Spectrometry (GC-MS) Multivariate Analysis (MVA) Partial Least Squares (PLS-DA). Further improvement technique vigorous shaking was tested analyzed. Approach: Cross-validation response permutation test PLS-DA S-plot Orthogonal Discriminant (OPLS-DA) applied to extracellular data Lactococcus lactis, which analyzed by...

The fresh leaves of Mitragyna speciosa (Korth.) Havil. have been traditionally consumed for centuries in Southeast Asia its healing properties. Although the alkaloids M . studied since 1920s, comparative and systematic studies metabolite composition based on different leaf maturity levels are still lacking. This study assessed secondary two stages (young mature) , using an untargeted liquid chromatography-electrospray ionisation-time-of-flight-mass spectrometry (LC-ESI-TOF-MS) profiling....

Fruit ripening is a complex phenomenon involving series of biochemical, physiological and organoleptic changes. Ripening process in mangosteen (Garcinia mangostana Linn.) unique which the fruit will only ripen properly if harvested during its middle stage (emergence purple/pink colour) but not earlier (green stage). The knowledge on molecular mechanism regulation behind this still limited. Hence, electrospray ionization liquid chromatography mass spectrometry (ESI-LC-MS) based metabolomics...

We have recently shown that the tocotrienol-rich fraction (TRF) of palm oil, a mixture vitamin E analogs, improves amyloid pathology in vitro and vivo. However, precise mechanisms remain unknown. In this study, we examined effects long-term (10 months) TRF treatment on behavioral impairments brain metabolites (15 months old) AβPP/PS1 double transgenic (Tg) Alzheimer’s disease (AD) mice. The open field test, Morris water maze, novel object recognition tasks revealed improved exploratory...

Gas chromatography mass spectrometry (GC-MS) and headspace gas (HS/GC-MS) were used to study metabolites produced by Lactococcus lactis subsp. cremoris MG1363 grown at a temperature of 30 °C with without agitation 150 rpm, 37 agitation. It was observed that L. more organic acids under Primary alcohols, aldehydes, ketones polyols identified as the corresponding trimethylsilyl (TMS) derivatives, whereas amino acids, including fatty detected through methyl chloroformate derivatization. HS...

Mitragyna speciosa is a psychoactive plant known as “ketum” in Malaysia and “kratom” Thailand. This distinctly to produce two important alkaloids, namely mitragynine (MG) 7-hydroxymitragynine (7-OH-MG) that can bind opioid receptors [1]. MG was reported exhibit antidepressant properties animal studies [2]. These compounds were also proposed have the potential replace analgesics with much lower risks of side effects [3]. To date, there are only over 40 metabolites identified M. [4,5]. obtain...

Hybridization is key to the evolution and diversity of plants in nature. Nepenthaceae comprises a family diverse tropical carnivorous pitcher plant species with extensive hybridization. However, there no study date on metabolite expression hybrids this family. We performed non-targeted metabolomics analysis pitchers two Nepenthes different dietary habits, namely, semi-detritivorous N. ampullaria rafflesiana their hybrid ( N . × hookeriana ) for comparative study. The whole-pitcher samples...

Aberrant gut microbiota dysbiosis in women with a previous history of gestational diabetes mellitus (post-GDM) was comparable to that adults type 2 (T2DM). Nonetheless, potential relationships between diet, microbiota, and metabolic phenotypes post-GDM after delivery are yet be discovered. In this research, we assessed the relationship macronutrient intakes, composition, (i.e., anthropometrics glycemic control) without postpartum glucose intolerance (GI). About 24 were included study, 14...

Systems biology is an interdisciplinary study that involves a combination of expertise in biology, chemistry, mathematics, physics, and engineering to unravel the complex living systems by incorporating multiple kinds quantitative molecular computations using sophisticated mathematical models. This can be applied identify understand metabolic changes recalcitrant plant species. Many tropical plants with seeds have difficulty long-term seed storage preservation due their intolerance...

Garcinia mangostana Linn.(mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products.However, reports detailing its characterization metabolite content are still scarce.Most of the earlier only focused on extraction specific such as xanthones anthocyanins, rather than a comprehensive metabolome profile.In this study, global metabolomics approach using gas chromatography-mass...

In solving the issue of basal stem rot diseases caused by Ganoderma, an investigation Scytalidium parasiticum as a biological control agent that suppresses Ganoderma infection has gained our interest, it is more environmentally friendly. Recently, fungal co-cultivation emerged promising method to discover novel antimicrobial metabolites. this study, established technique co-culturing and boninense was applied produce induce metabolites have antifungal activity against G. boninense. The crude...

Hybridisation plays a significant role in the evolution and diversification of plants. among Nepenthes species is extensive, either naturally or man-made. To investigate effects hybridisation on chemical compositions, we carried out metabolomics study pitcher tissue ampullaria, rafflesiana their hybrid, × hookeriana. Pitcher samples were harvested extracted methanol:chloroform:water via sonication-assisted extraction before analysed using LC-TOF-MS. MS data XCMS online version 2.2.5. This...

Agarwood is the highly valuable fragrant resin of wounded Aquilaria spp. trees widely used in fragrances, medicines and incenses. Among spp., A. malaccensis primary producer mainly found Indonesia Malaysia. In normal condition, agarwood naturally formed as a defense mechanism upon physical damage or microbial infection on trees, which slow process that occurs over several years. The high demand has spurred development various artificial inoculation methods where formation synthetically...

Chrysopogon zizanioides (L.) Roberty, or vetiver grass, is a deep-rooted perennial grass. An examination of allelopathy using metabolomic approach offers valuable insights into extract's mechanism action and phytotoxicity. This study utilised an untargeted metabolomics through the use choisum (Brassica rapa L. subsp. chinensis Hanelt var. parachinensis (L.H. Bailey) Hanelt) as target plant because it easier to cultivate susceptible extract. Vetiver root methanolic extracts at various...