A5058340269- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
- Periodontal Regeneration and Treatments
- Cancer, Hypoxia, and Metabolism
- Mesenchymal stem cell research
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Radiomics and Machine Learning in Medical Imaging
- Nanoparticle-Based Drug Delivery
- Angiogenesis and VEGF in Cancer
- Advanced Drug Delivery Systems
- Prostate Cancer Treatment and Research
- Mathematical Biology Tumor Growth
- Synthesis and Characterization of Pyrroles
- Proteins in Food Systems
- Digestive system and related health
- Nanoplatforms for cancer theranostics
- Temporomandibular Joint Disorders
- Cancer Immunotherapy and Biomarkers
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Bone fractures and treatments
- Bone and Dental Protein Studies
Université de Strasbourg
2016-2025
Regenerative NanoMedicine
2016-2025
Inserm
2012-2025
Hôpitaux Universitaires de Strasbourg
2017-2019
Immuno-Rhumathologie moléculaire
2017
Institut de Recherche en Cancérologie de Montpellier
2017
Université de Montpellier
2012
Background Morphogenesis results from the coordination of distinct cell signaling pathways controlling migration, differentiation, apoptosis, and proliferation, along stem/progenitor dynamics. To decipher this puzzle, we focused on epithelial-mesenchymal transition (EMT) "master genes". EMT has emerged as a unifying concept, involving cell-cell adhesion, migration apoptotic pathways. also appears to mingle with stemness. However, very little is known physiological role relevance...
The impact of cancer on lifespan is significantly increasing worldwide. Enhanced activity drug efflux pumps and the incidences tumor microenvironment such as apparition a hypoxic gradient inside bulk tumor, are major causes chemotherapy failure. For instance, expression Hypoxia-inducible factor (HIF-1α) has been associated with metastasis, resistance to reduced survival rate. One current challenges fight against therefore find new molecules therapeutic potential that could overcome this...
Abstract The extended life expectancy and the raise of accidental trauma call for an increase osteoarticular surgical procedures. Arthroplasty, main clinical option to treat lesions, has limitations drawbacks. In this manuscript, we test preclinical safety innovative implant ARTiCAR treatment lesions. Thanks combination two advanced therapy medicinal products, a polymeric nanofibrous bone wound dressing marrow-derived mesenchymal stem cells, promotes both subchondral cartilage regeneration....
Patient-derived tumoroid (PDT) has been developed and used for anti-drug screening in the last decade. As compared to other existing drug models, a PDT-based vitro 3D cell culture model could preserve histological mutational characteristics of their corresponding tumors mimic tumor microenvironment. However, few studies have carried out improve microvascular network connecting PDT its surrounding microenvironment, knowing that poor tumor-selective transport delivery is one major reasons both...
Abstract: In tissue engineering, it is still rare today to see clinically transferable strategies for tissue-engineered graft production that conclusively offer better regeneration than the already existing technologies, decreased recovery times, and less risk of complications. Here a novel tissue-engineering concept presented living bone implants combining 1) nanofibrous microporous implant as cell colonization matrix 2) 3D spheroids. This combination, double implants, shows clinical...
Aim: Success of functional vascularized tissue repair depends on vascular support system supply and still remains challenging. Our objective was to develop a nanoactive implant enhancing endothelial cell activity, particularly for bone engineering in the regenerative medicine field. Materials & methods: We developed new strategy tridimensional based cell-dependent sustained release VEGF nanoparticles. These nanoparticles were homogeneously distributed within nanoreservoirs onto porous...
Current approaches of regenerative therapies constitute strategies for bone tissue reparation and engineering, especially in the context genetical diseases with skeletal defects. Bone regeneration using electrospun nanofibers' implant has following objectives: neoformation induction rapid healing, reduced postoperative complications, improvement quality. In vivo implantation polycaprolactone (PCL) biomembrane functionalized BMP-2/Ibuprofen mouse maxillary defects was followed by kinetics...
This work describes a patient-derived tumoroid model (PDTs) to support precision medicine in lung oncology. The use of human adipose tissue-derived microvasculature and peripheral blood mononuclear cells (PBMCs) permits achieve physiologically relevant tumor microenvironment. study involved ten patients at various stages progression. vascularized, immune-infiltrated PDT could be obtained within two weeks, matching the requirements therapeutic decision. Histological transcriptomic analyses...
Aim: We developed polymeric membranes for local administration of nonsoluble anti-inflammatory statin, as potential wound patch in rheumatic joint or periodontal lesions. Methods: Electrospun polycaprolactone were fitted with polysaccharide-atorvastatin nanoreservoirs by using complexes poly-aminocyclodextrin. Characterization methods are UV-Visible and X-ray photoelectron spectroscopy, molecular dynamics, scanning transmission electron microscopy. In vitro, seeded macrophages, inflammatory...
Glioblastoma (GBM) is one of the most aggressive types cancer, which begins within brain. It invasive type glioma developed from astrocytes. Until today, Temozolomide (TMZ) only standard chemotherapy for patients with GBM. Even though extends survival patients, there are many undesirable side effects, and cases show resistance to TMZ. FL3 a synthetic flavagline displays potent anticancer activities, known inhibit cell proliferation, by provoking cycle arrest, leads apoptosis in lot cancer...
The sensory innervation of the dental pulp is essential for tooth function and protection. It mediated by axons originating from trigeminal ganglia spatio-temporally regulated. We have previously shown that bioengineered teeth can be achieved only under immunosuppressive conditions. aim this study was to develop a model determine role Semaphorin 3A (Sema3A) in teeth. first analysed mandibular molars newborn (postnatal day 0: PN0) mice deficient Sema3A (Sema3A−/−), strong inhibitor axon...
The treatment of osteochondral defects remains a challenge. Four scaffolds were produced using Food and Drug Administration (FDA)-approved polymers to investigate their therapeutic potential for the regeneration unit. Polycaprolactone (PCL) poly(vinyl-pyrrolidone) (PVP) made by electrohydrodynamic techniques. Hydroxyapatite (HAp) and/or sodium hyaluronate (HA) can be then loaded PCL nanofibers PVP particles. purpose adding hydroxyapatite into PCL/PVP is increase regenerative ability...
One major limitation for the vascularization of bone substitutes used filling is presence mineral blocks. The newly-formed blood vessels are stopped or have to circumvent blocks, resulting in inefficient delivery oxygen and nutrients implant. This leads necrosis within implant poor engraftment substitute. aim present study provide a substitute currently clinic with suitably guided properties. therapeutic hybrid filling, containing polymeric component, fortified pro-angiogenic smart...
Transplantation of bone marrow mesenchymal stem cells (BMDCs) into a denervated side the spinal cord was reported to be useful option for axonal regeneration. The innervation teeth is essential their function and protection but does not occur spontaneously after injury. Cultured reassociations between dissociated embryonic dental epithelial implantation lead vascularized tooth organ However, when were coimplanted with trigeminal ganglion (TG), did occur. On other hand, mixed marrow–derived...
Radiation therapy and platinum-based chemotherapy are common treatments for lung cancer patients. Several factors considered the low overall survival rate of cancer, such as patient's physical state complex heterogeneity tumor, which leads to resistance treatment. Consequently, precision medicines needed patients improve their quality life. Until now, no patient-derived tumoroid model has been reported predict efficiency radiation in non-small-cell cancer. Using our model, we report that...
The time needed to obtain functional regenerated bone tissue depends on the existence of a reliable vascular support. Current techniques used in clinic, for example after tooth extraction, do not allow regaining or preserving same volume. Our aim is develop cellularized active implant third generation, equipped with human mesenchymal stem cells improve quality vascularization. We seeded commercialized collagen (hMSCs) and then umbilical vein endothelial (HUVECs). analyzed biocompatibility...
The fundamental purpose of this study was to develop a stable lyophilised finasteride nanosystem (FNS-NS) for topical delivery. FNS-NS fabricated using an ultrasonication technique. impact two different cryoprotectants on the physicochemical characteristics before and after lyophilisation thoroughly investigated. had spherical shape with particle size lied between 188.6 nm ± 4.4 298.7 4.7, low PDI values (0.26 0.02 0.32 0.02) zeta potential ranging from -38.3 +53.3 mV. confocal laser...
Abstract We here describe a prototype of patient-derived tumoroid that prefigures the precision medicine approach by helping experimental assessment response to current treatments. The introduction microvessels help forming tumor-connected vasculature, and peripheral blood immune cells was shown be essential for representativeness model. study is based on cohort 11 patients at various stages disease. Noteworthy, this predictive vascularized, immunocompetent micromodel can obtained within 2...