A5009516957- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Electrospun Nanofibers in Biomedical Applications
- Biomedical Ethics and Regulation
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Genetic factors in colorectal cancer
- 14-3-3 protein interactions
- Mitochondrial Function and Pathology
- Birth, Development, and Health
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
Cliniques Universitaires Saint-Luc
2023
UCLouvain
2015-2022
STUDY QUESTIONDo primordial-primary versus secondary follicles embedded inside a fibrin matrix have different capabilities to survive and grow after isolation transplantation?
What is the best source of ovarian cells for artificial ovary: medulla or cortex, cryopreserved fresh? Ovarian from fresh medullary tissue, which can be isolated in larger numbers, show higher viability and are able to improve graft vascularization. In a previous study, addition endothelial along with was found crucial formation well-vascularized ovary-like structure. This study first evaluate both effect cryopreservation tissue on cells. Prospective experimental an academic research unit...
Fertility preservation research in women today is increasingly taking advantage of bioengineering techniques to develop new biomimetic materials and solutions safeguard ovarian cell function microenvironment
Ovarian tissue cryopreservation followed by transplantation after cancer remission is the most commonly applied fertility restoration approach in very young girls and women who require immediate therapy. However, clinicians strongly advise against reimplantation of one's own ovarian when there a high risk recurrence grafting. For these patients, development an alternative strategy, namely transplantable artificial ovary, offers future hope conceiving. The first essential requirement for...
Abstract To successfully assemble a bio‐engineered ovary, we need to create three‐dimensional matrix able accommodate isolated follicles and cells. The goal of this study was develop an extracellular hydrogel (oECM) derived from decellularized bovine ovaries support, in combination with alginate, human ovarian follicle survival growth vitro. Two different hydrogels (oECM1, oECM2) were produced compared terms decellularization efficiency (dsDNA), ECM preservation (collagen glycosaminoglycan...
Are mouse preantral follicles differently affected by isolation, encapsulation and/or grafting procedures according to stage?Isolated secondary showed superior ability survive and grow after transplantation, which was not related a particular effect of the isolation procedure, but rather their own induce neoangiogenesis.Isolated encapsulated can (6-27%) (80-100%) in fibrin matrix with low concentration fibrinogen thrombin (F12.5/T1) short-term transplantation.An vivo experimental model using...
(1) Background: Ovarian tissue transplantation with adipose tissue-derived stem cells (ASCs) has been shown to enhance graft vascularization and increase follicle survival after a short interval of 7 days. The aim the present study was investigate their long-term effects on primordial pool maintenance development. (2) Methods: A total 14 severe combined immunodeficient (SCID) mice were grafted frozen-thawed human ovarian or without ASCs. Blood taken monthly in order quantify anti-Müllerian...
To support survival and growth of follicles, the transplantable artificial ovary should mimic original organ, offering a physical (3D matrix) biological (cells). In order to replicate ovarian cell populations, aim this study is assess proportions stromal endothelial cells in cortex. end, biopsies were obtained from six women (mean age: 49 years). The epithelial layer medulla carefully removed. cortex was finely minced enzymatically digested isolated fixed. For characterization,...
To support survival and growth of follicles, the transplantable artificial ovary should mimic original organ, offering a physical (3D matrix) biological (cells). In order to replicate ovarian cell populations, aim this study is assess proportions stromal endothelial cells in cortex. end, biopsies were obtained from six women (mean age: 49 years). The epithelial layer medulla carefully removed. cortex was finely minced enzymatically digested isolated fixed. For characterization,...
Abstract Study question Does ovarian tissue transplantation affect extracellular matrix (ECM) components? Summary answer Ovarian affects ECM components in a time- and spatial-dependent manner What is known already Human subjected to specific pattern of remodeling during woman’s life. Such changes have crucial influence on folliculogenesis. Ischemia-reperfusion injury after also leads the ECM, such as occurrence fibrotic areas. However, there no in-depth characterization this yet or any study...