A5056097369- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Plant tissue culture and regeneration
- Epigenetics and DNA Methylation
- Parkinson's Disease Mechanisms and Treatments
- Cancer Risks and Factors
- Renal and related cancers
- Genetic and phenotypic traits in livestock
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Neurological disorders and treatments
- Seed Germination and Physiology
- Allelopathy and phytotoxic interactions
Universidade Estadual do Ceará
2021-2024
Universidade Potiguar
2018
This study aimed to verify the effect of GnRH at time artificial insemination and Dominant follicle (DF) diameter on Pregnancy rate (PR). 384 multiparous upgraded Murrah breed buffalo within 30 60 days postpartum with a body condition score 3.4±0.6 were used. The synchronization protocol consisted day 0 (D0) intravaginal device 1g P4, application 2.0mg BE, administration 10 ml injectable mineral supplementation 4 pm. On D9, pm, was removed, injected 0.265 mg PGF2α 300 IU eCG Intramuscular...
ABSTRACT This study assessed the potential protective effects of Mangiferin (MANGI) at various concentrations (10, 50 and 100 μM) on follicular survival activation, enzyme activity catalase (CAT) superoxide dismutase (SOD), as well relative mRNA expression for BAX , BCL2 SOD in porcine ovarian tissue cultured vitro 48 h with without chemical stress induced by 0.3 μg/mL doxorubicin (DOX). The results indicated that when MANGI was associated DOX, regardless tested concentration, increased ( p...
In brief Conditioned medium from Wharton’s jelly mesenchymal stem cells improved tissue and preantral follicle outcomes, preventing adverse effects of oxidative stress, apoptosis, epigenetic changes. Abstract This study investigated the methylation patterns H3K4me3 H3K9me3, as well mRNA expression genes encoding regulators KDM1AX1 , KDM1AX2 KDM3A in goat follicles developed vivo (Uncultured control) or after vitro culture for 7 days either absence (α-MEM + ) presence conditioned CM)...
Carvacrol (C10H14O), an efficient phenolic antioxidant substance for several cell types, may become a useful female germ cells and embryo culture. This study investigates the effects of carvacrol supplementation on bovine oocytes in vitro maturation (IVM) production. In total, 1222 cumulus-oocyte complexes were cultured TCM-199+ alone (control treatment) or supplemented with at concentrations 3 µM (Carv-3), 12.5 (Carv-12.5), 25 (Carv-25). After IVM, subjected to fertilization production,...
This study evaluated the effects of different concentrations (10, 20, or 40 μM) eugenol (EUG 10, EUG 40), ascorbic acid (50 μg/mL; AA) anethole (300 ANE 300) on in-vitro survival and development goat preantral follicles oxidative stress in cultured ovarian tissue. Ovarian fragments from five goats were for 1 7 days Alpha Minimum Essential Medium (α-MEM+) supplemented not with AA, 300, 20 40. On day culture, when compared to MEM, addition had increased rate follicular development, as observed...
This study assessed the histones methylation profile (H3K4me3 and H3K9me3) in late preantral (PA) early antral (EA) caprine follicles grown vivo vitro, anethole effect during vitro culture of PA follicles. Uncultured vivo-grown (PA, n = 64; EA, 73) were used as controls to assess genes' expression related apoptosis cascade (BAX, proapoptotic; BCL2, antiapoptotic), steroidogenesis (CYP17, CYP19A1), demethylation (KDM1AX1, KDM1AX2, KDM3A). The isolated (n 174) cultured for 6 days α-MEM+ either...
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Considerando o impacto que estresse oxidativo (EO) e as alterações epigenéticas podem exercer sobre a foliculogênese, melhor entendimento desses eventos nos auxiliam na busca da eficiência do cultivo in vitro (CIV) de folículos ovarianos em diversas espécies. Diante disso, objetivo dessa revisão é abordar principais características avanços CIV folicular, expondo suas limitações (ex. EO epigenéticas) alternativas antioxidantes meio condicionado células-tronco mesenquimais - MC-CTMs). Para...
This study aimed to examine the in vitro culture of secondary preantral follicles, using reused ovaries, compare both 2D and 3D methods system medium replacement. Twenty-five pairs ovaries from mixed-breed goats were used for experiment. Follicular puncture antral follicles was performed production. After this procedure, submitted a microdissection procedure. The isolated randomly divided into three treatments: (a) Two-dimensional with partial replacement during (2D PR), (b)...
To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, to evaluate effects Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating (FSH) on vitro development steroid production isolated porcine preantral follicles. Porcine secondary follicles were cultured NCSU23 α-MEM media for 4 days. Once was determined be optimal culture supplemented FSH (1.5 ng/mL), AMH (50 ng/mL) two hormones....