A5037778356- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Hypothalamic control of reproductive hormones
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Parasite Biology and Host Interactions
- RNA modifications and cancer
- Fish Ecology and Management Studies
- Identification and Quantification in Food
- Gene expression and cancer classification
- Fish Biology and Ecology Studies
- Diet and metabolism studies
- Aquaculture Nutrition and Growth
- Sperm and Testicular Function
- T-cell and B-cell Immunology
- Myxozoan Parasites in Aquatic Species
- Proteoglycans and glycosaminoglycans research
- CRISPR and Genetic Engineering
- Clinical Nutrition and Gastroenterology
- Glycosylation and Glycoproteins Research
University of Victoria
2005-2023
Simon Fraser University
2013-2018
We report 80,388 ESTs from 23 Atlantic salmon ( Salmo salar ) cDNA libraries (61,819 ESTs), 6 rainbow trout Oncorhynchus mykiss (14,544 2 chinook tshawytscha (1317 sockeye nerka (1243 and lake whitefish Coregonus clupeaformis (1465 ESTs). The majority of these are 3′ sequences, allowing discrimination between paralogs arising a recent genome duplication in the salmonid lineage. Sequence assembly reveals 28,710 different S. , 8981 O. 1085 520 1176 C. putative transcripts. annotate submitted...
Abstract Background We have developed and fabricated a salmonid microarray containing cDNAs representing 16,006 genes. The genes spotted on the array been stringently selected from Atlantic salmon rainbow trout expressed sequence tag (EST) databases. EST databases presently contain over 300,000 sequences 175 cDNA libraries derived wide variety of tissues different developmental stages. In order to evaluate utility microarray, number hybridization techniques screening methods tested. Results...
Salmonids are one of the most intensely studied fish, in part due to their economic and environmental importance, a recent whole genome duplication common ancestor salmonids. This greatly impacts species diversification, functional specialization, adaptation. Extensive new genomic resources have recently become available for Atlantic salmon (Salmo salar), but documentation allelic versus duplicate reference genes remains major uncertainty complete characterization its evolution.From existing...
Salmonids are of interest because their relatively recent genome duplication, and extensive use in wild fisheries aquaculture. A comprehensive gene list a comparison genes some the different species provide valuable genomic information for one most widely studied groups fish. 298,304 expressed sequence tags (ESTs) from Atlantic salmon (69% total), 11,664 chinook, 10,813 sockeye, 10,051 brook trout, 10,975 grayling, 8,630 lake whitefish, 3,624 northern pike ESTs were obtained this study have...
The northern pike is the most frequently studied member of Esociformes, closest order to diverse and economically important Salmoniformes. ancestor all salmonids purportedly experienced a whole-genome duplication (WGD) event, making salmonid species ideal for studying early impacts genome while complicating their use in wider analyses teleost evolution. Studies suggest that Esociformes diverged from lineage prior WGD, supporting as pre-duplication outgroup. Here we present first assembly,...
Abstract Background The sablefish (order: Scorpaeniformes) is an economically important species in commercial fisheries of the North Pacific and emerging aquaculture. Aside from a handful sequences NCBI few published microsatellite markers, little known about genetics this species. development genetic tools, including polymorphic markers linkage map will allow for successful future broodstock mapping phenotypes interest. significant sexual dimorphism between females males makes test early...
Piscirickettsia salmonis is the intracellular bacterium that causes salmonid rickettsial septicemia, an infectious disease kills millions of farmed fish each year. The mechanisms used by P. to survive and replicate within host cells are not known. Piscirickettsiosis severe necrosis hematopoietic kidney. Microarray-based experiments with QPCR validation were identify Atlantic salmon macrophage kidney genes differentially transcribed in response infection. Infections confirmed microscopy...
Development and maturation of the ovary requires precisely coordinated expression specific gene classes to produce viable oocytes. We undertook identification some genes involved in these processes by creating ovary-specific cDNA libraries suppression subtractive hybridization microarray-based analyses. present 5778 tissue- sex-specific from subtracted testis libraries, many which remain unidentified. A microarray containing 3557 salmonid cDNAs was used compare transcriptomes precocious at...
Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain IgH locus possesses two parallel isoloci IGH-A and IGH-B ), that are related to the genomic duplication event in family Salmonidae. These duplicated loci provide a unique opportunity examine mechanisms of genome diversity evolution vertebrates. In this study, we defined structure these salmon, sequenced 24 bacterial artificial chromosome (BAC) clones were assembled into (1.1 Mb) (0.9 loci. addition, over 7,000...
Nuclear deoxyribonucleic acid sequences from approximately 15,000 salmon louse expressed sequence tags (ESTs), the complete mitochondrial genome (16,148bp) of louse, and 16S ribosomal ribonucleic (rRNA) cytochrome oxidase subunit I (COI) genes 68 lice collected Japan, Alaska, western Canada support a Pacific lineage Lepeophtheirus salmonis that is distinct occurring in Atlantic Ocean. On average, nuclear are 3.2% different, 7.1% rRNA COI 4.2% 6.1% respectively. Reduced genetic diversity...
Sea lice are common parasites of both farmed and wild salmon. Salmon farming constitutes an important economic market in North America, South Northern Europe. Infections with sea can result significant production losses. A compilation genomic information on different genera is resource for understanding their biology as well the study population genetics control strategies. We report over 150,000 expressed sequence tags (ESTs) from five species (Pacific Lepeophtheirus salmonis (49,672 new...
Abstract Background The products of cyp19 , dax foxl2 mis sf1 and sox9 have each been associated with sex-determining processes among vertebrates. We provide evidence for expression these regulators very early in salmonid development tissues outside the hypothalamic-pituitary-adrenal/gonadal (HPAG) axis. Although function factors sexual differentiation defined, their roles before fate decisions beyond brain or gonad are essentially unknown. Results Bacterial artificial chromosomes containing...
Abstract Morphogens are developmental regulators that modulate different tissue patterning, proliferation, differentiation, or remodeling processes in embryonic and adult tissues. may also evoke specific regulatory programs stem cells. Some of the morphogens involved these have been characterized, while others remain unidentified. A microarray containing 3,557 salmonid cDNAs was used to compare transcriptomes rainbow trout precocious ovary at three stages during second year (June, August,...
Abstract Background Growth hormone (GH) is an important regulator of skeletal growth, as well other adapted processes in salmonids. The GH gene ( gh ) salmonids represented by duplicated, non-allelic isoforms designated gh1 and gh2 . We have isolated characterized -containing bacterial artificial chromosomes (BACs) both Atlantic Chinook salmon Salmo salar Oncorhynchus tshawytscha order to further elucidate our understanding the conservation regulation these loci. Results BACs containing from...
The GnRH gene is transcribed in both the brain and gonads. critical for reproduction, but function importance of ovary testis not clear. In this study we examine whether regulation distinct gonads, gonads altered after genome duplication, regulatory region tightly conserved vertebrates. From testis, isolated sequenced first time two different genes their complementary DNAs that encode identical peptide known as salmon GnRH. Rainbow trout were selected because they are tetraploid due to...
GnRH is usually classified as a neuropeptide that synthesized in the brain. Recent evidence indicates mRNA present also ovary and testis. However, isolation of peptide from testis has not been reported. We used HPLC specific RIAs to determine whether can be detected gonads, developmental stage at which expressed, number molecular forms are Extracts immature mature ovarian testicular tissue were examined 17- 21-mo-old rainbow trout (Oncorhynchus mykiss). For first time, peptides isolated...
Abstract Genomic resources in rainbow smelt ( Osmerus mordax ) enable us to examine the genome duplication process salmonids and test hypotheses relating fate of duplicated genes. They further pursue physiological ecological studies smelt. A bacterial artificial chromosome library containing 52,410 clones with an average insert size 146 kb was constructed. This represents 11-fold coverage O. genome. In addition, several complementary deoxyribonucleic acid libraries were constructed, 36,758...