A5071578637- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Biofuel production and bioconversion
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- Cancer Research and Treatments
- Salmonella and Campylobacter epidemiology
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Respiratory viral infections research
- bioluminescence and chemiluminescence research
- Biochemical and Structural Characterization
- Milk Quality and Mastitis in Dairy Cows
- Polyamine Metabolism and Applications
Ghent University
2019-2025
Ghent University Hospital
2025
Autonomous University of Queretaro
2024
Hospital General de México
2024
Purdue University West Lafayette
2022-2023
Moffitt Cancer Center
2023
Instituto de Productos Lácteos de Asturias
2012-2021
Instituto de Investigación Sanitaria del Principado de Asturias
2019-2021
Consejo Superior de Investigaciones Científicas
2012-2020
United Nations Development Programme
2020
Biofilms are a common cause of food contamination with undesirable bacteria, such as pathogenic bacteria. Staphylococcus aureus is one the major bacteria causing food-borne diseases in humans. A study designed to determine presence S. on contact surfaces dairy, meat, and seafood environments identify coexisting microbiota has therefore been carried out. total 442 samples were collected, was confirmed 6.1% samples. Sixty-three isolates recovered typed by random amplification polymorphic DNA...
Staphylococcal biofilms are a major concern in both clinical and food settings because they an important source of contamination. The efficacy established cleaning procedures is often hindered due to the ability some antimicrobial compounds induce biofilm formation, presence persister cells, small bacterial subpopulation that exhibits multidrug tolerance. Phage lytic enzymes have demonstrated activity against planktonic sessile bacteria. However, their lyse and/or select cells remains...
ABSTRACT Phage therapy is a promising option for fighting against staphylococcal infections. Two lytic phages, vB_SauM_phiIPLA-RODI (phiIPLA-RODI) and vB_SepM_phiIPLA-C1C (phiIPLA-C1C), belonging to the Myoviridae family exhibiting wide host ranges, were characterized in this study. The complete genome sequences comprised 142,348 bp 140,961 contained 213 203 open reading frames, respectively. gene organization was typical of Spounavirinae members, with long direct terminal repeats (LTRs),...
Staphylococcus epidermidis and aureus are important causative agents of hospital-acquired infections bacteremia, likely due to their ability form biofilms. The production a dense exopolysaccharide (EPS) matrix enclosing the cells slows penetration antibiotic down, resulting in therapy failure. EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed Escherichia coli characterized. A dose dependent but time independent response observed after treatment...
Bacteriophages have been proven as effective antimicrobial agents in the treatment of infectious diseases and other biocontrol applications including food preservation disinfection. The extensive use bacteriophages requires improved methodologies for medium- long-term storage well easy shipping. To this aim, we determined stability four Staphylococcus phages (phiIPLA88, phiIPLA35, phiIPLA-RODI phiIPLA-C1C) with potential at different temperatures (20°C/25°C, 4°C, -20°C, -80°C, -196°C) during...
Bacteria found in diverse ecosystems grow a community of aggregated cells that favors their survival and colonization. Different extracellular polymeric substances are used to entrap this multispecies forming biofilm, which can be associated biotic abiotic surfaces. This widespread successful way bacterial life, however, lead negative effects for human activity since many pathogen spoiling bacteria form biofilms not easy eradicate. Therefore, the search novel anti-biofilm bio-active...
Abstract Background Staphylococcus epidermidis is a commensal bacterium but can colonize the hospital environment due to its ability form biofilms favouring adhesion host tissues, medical devices and increasing resistance antibiotics. In this context, use of phages destroy an interesting alternative. Results The complete genomes two bacteriophages, vB_SepiS-phiIPLA5 vB_SepiS-phiIPLA7, have been analyzed. Their are 43,581 bp 42,123 bp, contain 67 59 orf s. Bioinformatic analyses enabled...
Abstract Staphylococcus aureus is considered a priority pathogen due to its increasing acquisition of antibiotic resistance determinants. Additionally, this microbe has the ability form recalcitrant biofilms on different biotic and inert surfaces. In context, bacteriophages their derived lytic proteins may be forward-looking strategy help combat staphylococcal biofilms. However, these antimicrobials exhibit individual limitations that overcome by combining them with other compounds. This...
The recent boom in phage therapy and biocontrol requires the design of suitable cocktails genetically different bacteriophages. current methods for typing phages need significant quantities purified DNA, may require a priori genetic information are cost time consuming. We have evaluated randomly amplified polymorphic DNA (RAPD)-PCR technique to produce unique reproducible band patterns from 26 bacteriophages infecting Staphylococcus epidermidis, aureus, Lactococcus lactis, Escherichia coli,...
Abstract Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain new generation lytic proteins, Artilysins, specific activity Gram-negative Here, we demonstrate that artilysation can also be used enhance the antibacterial bacteria and reduce dependence on external conditions. Art-240, chimeric protein anti-streptococcal endolysin λSa2lys polycationic peptide PCNP, shows similar...
ABSTRACT The use of bacteriophages as antimicrobials against pathogenic bacteria offers a promising alternative to traditional antibiotics and disinfectants. Significantly, phages may help remove biofilms, which are notoriously resistant commonly used eradication methods. However, the successful development novel antibiofilm strategies must take into account that real-life biofilms usually consist mixed-species populations. Within this context, study aimed explore effectiveness...
The elimination of bacterial biofilms remains a major challenge due to their recalcitrant nature. Bacteriophages, viruses that infect bacteria, have been gaining increasing attention as biofilm control agents. However, the development successful phage-based strategy requires in-depth analysis different parameters. It is particularly important determine ability given phage diffuse, propagate and remain viable within complex structure. Here, we examine some these properties for two...
Phage lysins are promising new therapeutics against multidrug-resistant bacteria. These so-called enzybiotics offer, amongst their most notable advantages, high target specificity and low resistance development. Moreover, there numerous recent ongoing studies aimed at demonstrating the efficacy safety of endolysins in animal models or even clinical trials. Nonetheless, as is case for other antimicrobials, it important to assess potential strategies that may broaden applications improve...
Phage lytic proteins are promising antimicrobials that could complement conventional antibiotics and help to combat multi-drug resistant bacteria cause important human animal infections. Here, we report the characterization of endolysin LysRODI (encoded by staphylophage phiIPLA-RODI) its application as a prophylactic mastitis treatment. The main properties were compared with those LysA72 phiIPLA35) chimeric protein CHAPSH3b (derived from virion-associated peptidoglycan hydrolase HydH5...