A5067824438- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Pharmacological Receptor Mechanisms and Effects
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience and Neuropharmacology Research
- Chemical Synthesis and Analysis
- Cytokine Signaling Pathways and Interactions
- Neurobiology and Insect Physiology Research
- Autophagy in Disease and Therapy
- Pain Mechanisms and Treatments
- Mosquito-borne diseases and control
- Ion Transport and Channel Regulation
- Nerve injury and regeneration
- Cancer, Stress, Anesthesia, and Immune Response
- Insect Pest Control Strategies
- Inflammatory mediators and NSAID effects
- Insect Resistance and Genetics
- Signaling Pathways in Disease
- Electrolyte and hormonal disorders
- Renin-Angiotensin System Studies
- NF-κB Signaling Pathways
- Cholesterol and Lipid Metabolism
- Insect and Arachnid Ecology and Behavior
- Cellular transport and secretion
- Viral Infectious Diseases and Gene Expression in Insects
National Centre of Scientific Research "Demokritos"
2015-2024
Institute of Biosciences & Applications
2012-2024
Howard Hughes Medical Institute
1990
Vanderbilt University
1990
National Hellenic Research Foundation
1985-1986
Ruhr University Bochum
1985
A lepidopteran insect cell-based expression system has been employed to express three Anopheles gambiae odorant receptors (ORs), OR1 and OR2, which respond components of human sweat, OR7, the ortholog Drosophila's OR83b, heteromerization partner all functional ORs in that system. With aid epitope tagging specific antibodies, efficient was demonstrated intrinsic properties proteins were revealed. Moreover, analysis orientation OR2 on cellular plasma membrane through use a novel 'topology...
Adenylyl cyclase activity was measured following labelling of the cellular ATP pool with [3H]adenine in intact Rat-1 fibroblasts that had been stably transfected to express murine delta-opioid receptor (clone D2). Basal [3H]cyclic AMP accumulation low and increased substantially by addition diterpene forskolin. The synthetic enkephalin D-Ala2,D-Leu5 (DADLE) produced strong inhibition forskolin-amplified production, whereas ligand ICI174864 augmented adenylyl activity. Naloxone unable mimic...
G protein‐coupled receptors (GPCRs) are a large family of cell surface that play critical role in nervous system function by transmitting signals between cells and their environment. They involved many, if not all, processes, dysfunction has been linked to various neurological disorders representing important drug targets. This overview emphasises the GPCRs system, which research focus members ERNEST COST action (CA18133) working group ‘Biological roles signal transduction’. First,...
A large body of evidence implicates the second and third intracellular loops carboxyl-terminal portion many G protein-coupled receptors as sites responsible for interaction to proteins. We synthesized a number peptides from selected murine delta-opioid receptor measured their ability modify ligand-stimulated protein activation 3H agonist binding receptor. In membranes Rat-1 fibroblasts transfected express stably (clone D2 cells), [D-Ser2-Leu5-Thr6]enkephalin (DSLET) stimulated high affinity...
Novel dermorphin tetrapeptides are described in which Tyr(1) is replaced by Dmt(1), where d-Ala(2) and Gly(4) N-methylated, Phe(3)-Gly(4) residue substituted the constrained Aba(3)-Gly(4) peptidomimetic. Most of these peptidic ligands displayed binding affinities nanomolar range for both μ- δ-opioid receptors but no detectable affinity κ-opioid receptor. Measurements cAMP accumulation, phosphorylation extracellular signal-regulated kinase (ERK1/2) HEK293 cells stably expressing each...
It remains unclear how opioid receptors (δ, μ, κ) are implicated in mechanisms controlling differentiation, cell proliferation, and survival. Opioid coupled to Gi/Go proteins recent findings have shown that can form a multicomponent signaling complex, consisting of members G protein the signal transducer activator transcription (STAT)5B. We thus wondered whether activation could direct differentiation neurite outgrowth through molecular pathway involving STAT5B other intermediates....
To explore the feasibility of developing inhibitors signaling by opioid receptors and other G protein-coupled (GPCRs) that use same protein pool, we investigated capacity a minigene encoding third intracellular loop δ-opioid receptor (δ-i3L) to act as competitive antagonist receptor-G interface interaction. In δ-i3L-expressing cells, peptide blocked high-affinity agonist binding both δ- μ-opioid (δ-OR μ-OR) attenuated α<sub>2</sub>-adrenergic (α2AR)-dependent...
Abstract Signal Transducers and Activators of Transcription (STATs) are transcription factors shown to be activated by G protein‐coupled receptors. In the present study, we demonstrate that acute morphine or [D‐Ala 2 ,N‐Me‐Phe 4 ,Gly 5 ‐ol]enkephalin (DAMGO) exposure COS‐7 cells transiently transfected with µ‐opioid receptor STAT5A, leads receptor‐dependent tyrosine phosphorylation STAT5A. Activation HEK293 cells, stably expressing agonists results in transcriptional activation a...
Solubilization of opioid receptors from rat cortical membranes that retained high-affinity guanine nucleotide-sensitive agonist binding was achieved using 10 mM CHAPS. We report the nature interactions mu and delta with nucleotide-binding protein G(o) by immunoprecipitation CHAPS extracts selective G(o)alpha-subunit antisera. Antiserum IM1 raised against amino acids 22-35 G(o)alpha selectively co-immunoprecipitated G(o)alpha-mu G(o)alpha-delta receptor complexes detected in...
Abstract The role of somatostatin and its mechanism action in the retina remains an important target for investigation. Biochemical pharmacological studies were engaged to characterize receptors rabbit retina, their coupling G‐proteins. ability selective ligands inhibit [ 125 I]Tyr11‐somatostatin‐14 binding retinal membranes was examined. sst 2 analogues SMS201–995, MK678, BIM23014, displayed IC 50 values 0.28 ± 0.12, 0.04 0.01 1.57 0.39 n m , respectively. 1 analogue CH275 moderately...