A5001594619- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Respiratory viral infections research
- Clostridium difficile and Clostridium perfringens research
- Ubiquitin and proteasome pathways
- Hepatitis C virus research
- RNA Research and Splicing
- interferon and immune responses
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Parasitic Infections and Diagnostics
- Inflammatory Myopathies and Dermatomyositis
- Infective Endocarditis Diagnosis and Management
- Systemic Sclerosis and Related Diseases
- Antibiotic Resistance in Bacteria
- Amoebic Infections and Treatments
- Nuclear Structure and Function
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Viral Infections and Vectors
- Liver Disease Diagnosis and Treatment
University of Parma
2014-2024
Istituto Superiore di Sanità
2004
Institut Jacques Monod
1997-2004
Université Paris Cité
1997-2004
Centre National de la Recherche Scientifique
1997-2000
The human gut microbiota is a dynamic community of microorganisms that undergo variable changes over the entire life span. To thoroughly investigate possible fluctuations throughout life, we performed pooled analysis healthy fecal samples across different age groups covering Our study integrated data from 79 publicly available studies and new stool an Italian cohort, i.e., Parma Microbiota project, resulting in 6,653 processed through shotgun metagenomic approach. This approach has allowed...
Virus detection and/or identification traditionally rely on methods based cell culture, electron microscopy and antigen or nucleic acid detection. These techniques are good, but often expensive time-consuming; furthermore, they not always lead to virus at the species type level. In this study, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) was tested as an innovative tool identify human polioviruses specific viral protein biomarkers in infected...
Accurate identification of Plasmodium infections in non-endemic countries is critical importance with regard to the administration a targeted therapy having positive impact on patient health and management allowing prevention risk re-introduction endemic malaria such countries. Malaria no longer Italy where it most commonly imported disease, one highest rates among European including France, UK Germany, prevalence 24.3% at University Hospital Parma. Molecular methods showed high sensitivity...
ObjectivesThe aim of this study was to determine the prevalence respiratory virus infections, including severe acute syndrome coronavirus 2 (SARS-CoV-2), during winter period December 2019 March 2020, via a tertiary care hospital-based survey in Parma, Northern Italy.MethodsA total 906 biological samples from tract were analysed by both conventional assays (including culture) and molecular targeting nucleic acids SARS-CoV-2 other viruses.ResultsOverall, 474 (52.3%) positive for at least one...
Surveillance of noroviruses in Italy identified the novel GII.17 human norovirus strain, Kawasaki 2014, February 2015. This strain emerged as a major cause gastroenteritis Asia during 2014/15, replacing pandemic GII.4 Sydney 2012, but being reported only sporadically elsewhere. is undergoing fast diversification and continuous monitoring important to understand evolution implement future strategies on vaccines.
Surveillance of norovirus infections in sporadic cases pediatric gastroenteritis admitted to a main hospital Northern Italy during full-year period (2002) showed that noroviruses (10.4%) were the second most common causative viral agent, following rotaviruses (21.1%), and (81%) mostly implicated mixed infections. The epidemic was September–December, with September November as months major prevalence (33.3 38.5%, respectively). Six distinct genotypes detected (GI.7, GII.1, GII.2, GII.4,...
Abstract In this study matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS), a reliable identification method for the diagnosis bacterial and fungal infections, is presented as an innovative tool to investigate protein profile cell cultures infected by most common viruses causing respiratory tract infections in humans. MALDI-TOF MS was applied influenza A B viruses, adenovirus C species, parainfluenza virus types 1, 2 3, syncytial virus, echovirus,...
The aim of this study was to assess the epidemiology intestinal parasitoses during a 5-year period in patients attending tertiary-care hospital non-endemic setting. In 2006–2010, 15,752 samples from 8,886 with clinically suspected parasitosis were subjected macroscopic and microscopic examination, parasitic antigen detection assays, cultures for protozoa nematodes. Real-time PCR assays differentiation Entamoeba histolytica E. dispar Dientamoeba fragilis also used. A statistical analysis...
Human cytomegalovirus (HCMV) is an opportunistic pathogen leading to severe and even fatal diseases in 'at-risk' categories of individuals upon primary infection or the symptomatic reactivation endogenous virus. The mechanisms which make virus able reactivate from latency are still matter intense study. However, very low number peripheral blood monocytes (an important latent reservoir) harbouring HCMV DNA makes it difficult obtain adequate viral quantities use such studies. Thus, aim present...
Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato (sl) complex transmitted to humans Ixodes ticks. B. sl complex, currently comprising at least 19 genospecies, includes the main pathogenic species responsible for human in Europe: stricto (ss), afzelii, and garinii. In this study, first time, MALDI-TOF MS was applied spp., supplementing existing database, limited ss, spielmanii garinii, with order enable identification of all potentially implicated LB...
Despite that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool in the clinical microbiology setting, few studies have till now focused on MALDI-TOF MS-based identification of dermatophytes. In this study, we analyze dermatophytes strains isolated from samples by MS to supplement reference database available our laboratory. Twenty four (13 and 11 field strains), identified both conventional molecular standard procedures,...
Detection of Entamoeba histolytica and its differentiation from dispar is an important goal the clinical parasitology laboratory. The aim this study was identification E. by MALDI-TOF MS, in order to evaluate application technique routine diagnostic practice. MS applied 3 amebic reference strains 14 isolated feces that had been differentiated molecular methods our Protein extracts cultures these (axenic for monoxenic field isolates) were analyzed spectra obtained statistical software. Five...
ABSTRACT We developed a nested reverse transcription-PCR (nRT-PCR) for the detection of noroviruses in stools, using random primers RT, JV12/JV13 primer pair first round nPCR, and set nine inner second, comprising sequences SR46, SR48, SR50, SR52, five novel oligonucleotide (113-1, 113-2, 115-1, 115-2, 115-3). The specificity nRT-PCR was confirmed by testing 61 stools containing enteric viruses other than noroviruses. In comparative assays on either or RNA dilutions from two genogroup I...