A5009233774- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Weed Control and Herbicide Applications
- Allelopathy and phytotoxic interactions
University of Johannesburg
2019-2020
Burkholderia andropogonis is the causal agent of bacterial leaf stripe, one three major diseases affecting Sorghum bicolor. However, biochemical aspects pathophysiological host responses are not well understood. An untargeted metabolomics approach was designed to understand molecular mechanisms underlying S. bicolor⁻B. interactions. At 4-leaf stage, two sorghum cultivars (NS 5511 and NS 5655) differing in disease tolerance, were infected with B. metabolic changes monitored over time. The...
Plant cell suspension culture systems are valuable for the study of complex biological such as inducible defence responses and aspects plant innate immunity. Perturbations to cellular metabolome can be investigated using metabolomic approaches in order reveal underlying metabolic mechanism responses. Lipopolysaccharides from sorghum pathogen, Burkholderia andropogonis (LPS
The use of plant cell suspension culture systems has demonstrated to be highly suitable for metabolomics investigations inducible defense responses. Here we report on sorghum cultures that were elicited with purified lipopolysaccharides from the pathogen Burkholderia andropogonis, activate metabolic pathways involved in chemical defenses plant. Metabolomic analysis using liquid chromatography coupled mass spectrometry identified a resorcinol phenolic lipid, annotated as sorgoleone, one...