Abstract Background Chlorogenic acids (CGAs) are a class of phytochemicals that formed as esters between different derivatives cinnamic acid and quinic molecules. In plants, accumulation these compounds has been linked to several physiological responses against various stress factors; however, biochemical synthesis differs from one plant another. Although structurally simple, the analysis CGA molecules with modern analytical platforms poses an challenge. The objective study was perform...

Moringa species are multipurpose plants with nutritional, medicinal and industrial benefits. The flavonoids content of oleifera ovalifolia was studied using an Ultra high-performance liquid chromatography coupled electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-qTOF-MS). results revealed that the two contain at least 17 compounds between them. However, M. found to most than which contained only three total flavonoids. Furthermore, all in were shown be...

The metabolome of a biological system provides functional readout the cellular state, thus serving as direct signatures biochemical events that define dynamic equilibrium metabolism and correlated phenotype. Hence, to elucidate processes involved in sorghum responses fungal infection, liquid chromatography-mass spectrometry-based untargeted metabolomic study was designed. Metabolic alterations three cultivars responding Colletotrichum sublineolum, were investigated. At 4-leaf growth stage,...

Tomato (Solanum lycopersicum) is an important dietary source of bioactive phytochemicals and active breeding programs constantly produce new cultivars possessing superior desirable traits. The phytopathogenic Ralstonia solanacearum, the causal agent bacterial wilt, a highly destructive disease with high economic impact on tomato production. This study followed untargeted metabolomic approach involving four aimed at identification secondary metabolites involved in plant defense after...

Plant growth–promoting rhizobacteria (PGPR) are beneficial microbes in the rhizosphere that can directly or indirectly stimulate plant growth. In addition, some prime plants for enhanced defense against a broad range of pathogens and insect herbivores. this study, four PGPR strains (Pseudomonas fluorescens N04, P. koreensis N19, Paenibacillus alvei T19, Lysinibacillus sphaericus T22) were used to induce priming Solanum lycopersicum (cv. Moneymaker) plants. Plants inoculated with each PGPRs,...

Untargeted metabolomic studies generate information-rich, high-dimensional, and complex datasets that remain challenging to handle fully exploit. Despite the remarkable progress in development of tools algorithms, “exhaustive” extraction information from these is still a non-trivial undertaking. A conversation on data mining strategies for maximal needed. Using liquid chromatography-mass spectrometry (LC-MS)-based untargeted dataset, this study explored influence collection parameters...

Bidens pilosa is an edible herb from the Asteraceae family which traditionally consumed as a leafy vegetable. B. has many bioactivities owing to its diverse phytochemicals, include aliphatics, terpenoids, tannins, alkaloids, hydroxycinnamic acid (HCA) derivatives and other phenylpropanoids. The later compounds such chlorogenic acids (CGAs), are produced either regio- or geometrical isomers. To profile CGA composition of pilosa, methanol extracts tissues, callus cell suspensions were utilized...

Microbial plant biostimulants have been successfully applied to improve growth, stress resilience and productivity. However, the mechanisms of action are still enigmatic, which is main bottleneck for fully realization implementation into agricultural industry. Here, we report elucidation a global metabolic landscape maize ( Zea mays L) leaves in response microbial biostimulant, under well-watered drought conditions. The study reveals that increased pool tricarboxylic acid (TCA)...

The rhizosphere is a highly complex and biochemically diverse environment that facilitates plant-microbe microbe-microbe interactions, this region found between plant roots the bulk soil. Several studies have reported root exudation metabolite secretion by rhizosphere-inhabiting microbes, suggesting these metabolites play vital role in interactions. However, biochemical constellation of soil yet to be fully elucidated thus remains extremely elusive. In regard, effects growth-promoting...

Drought is one of the major abiotic stresses causing severe damage and losses in economically important crops worldwide. decreases plant water status, leading to a disruptive metabolic reprogramming that negatively affects growth yield. Seaweed extract-based biostimulants show potential as sustainable strategy for improved crop health stress resilience. However, cellular, biochemical, molecular mechanisms governing agronomically observed benefits seaweed extracts on plants are still poorly...

The ineffective removal of antibiotic residues and their metabolites by conventional wastewater treatment setups lead to continuous discharge into the environment, causing risks both aquatic life human health. This study explored use a non-thermal plasma (NTP) generated atmospheric air for degradation cefixime (CFX), widely consumed recalcitrant antibiotic. achieved complete CFX using an NTP technology in 8 min time at 6 kV 20 kHz applied AC voltage frequency, respectively high alternating...

Abstract Spontaneous nephropathy in Bulgaria, which is observed frequently during meat inspection and differs morphologically from the classical description of mycotoxic porcine/chicken as made Denmark, was found to have a multi-mycotoxic aetiology being mainly provoked by combined effect ochratoxin A, penicillic acid fumonisin B1 addition not-yet-known metabolite. Mean contamination levels A were consecutively low (188.8 376.4 µg kg−1) contrast high (5564.1 3254.5 (838.6 904.9 for 2006...

Isonitrosoacetophenone (INAP, 2-keto-2-phenyl-acetaldoxime) is a novel inducer of plant defense. Oxime functional groups are rare in natural products, but can serve as substrates depending on existing secondary pathways. Changes the metabolomes sorghum and tobacco cells treated with INAP were investigated chemometric tools multivariate statistical analysis used to investigate changes metabolite distribution patterns resulting from elicitation. Liquid chromatography combined mass spectrometry...

Metabolomics is providing new dimensions into understanding the intracellular adaptive responses in plants to external stimuli. In this study, a multi-technology-metabolomic approach was used investigate effect of fungal sterol, ergosterol, on metabolome cultured tobacco cells. Cell suspensions were treated with different concentrations (0–1000 nM) ergosterol and incubated for time periods (0–24 h). Intracellular metabolites extracted two methods: selective dispersive liquid-liquid...

Centella asiatica is an important source of biologically active pentacyclic triterpenoids. The enhancement the biosynthesis centellosides by manipulation associated metabolic pathways receiving much attention. Jasmonates play critical roles in plant metabolism up-regulating expression genes related to secondary metabolites. Here, we investigated effect methyl jasmonate (MeJa) C. through targeted metabolomic profiling asiaticoside and madecassoside as well their aglycones, asiatic acid...

Moringa oleifera is a tree plant with important bioactive compounds that confer its pharmacological properties. These have been commonly extracted from the leaves using general methanol-based procedures. Organic solvents used for extraction purposes negative impacts on environment and human health. Green technologies are emerging as preferred choices it encourages practices environmentally friendly pose less or no harm to humans. In this study, extraction-ability of pressurized hot water,...

Solanum aculeastrum fruits are used by some cancer sufferers as a form of alternative treatment. Scientific literature is scarce concerning its anticancer activity, and thus the aim study was to assess in vitro P-glycoprotein inhibitory potential extracts S. fruits. Furthermore, assessment combinational effect with doxorubicin also done.The crude extract prepared ultrasonic maceration. Liquid-liquid extraction yielded one aqueous two organic fractions. Bioactive constituents were isolated...

Metabolomics has developed into a valuable tool for advancing our understanding of plant metabolism. Plant innate immune defenses can be activated and enhanced so that, subsequent to being pre-sensitized, plants are able launch stronger faster defense response upon exposure pathogenic microorganisms, phenomenon known as priming. Here, three contrasting chemical activators, namely acibenzolar-S-methyl, azelaic acid riboflavin, were used induce primed state in Nicotiana tabacum cells....

Burkholderia andropogonis is the causal agent of bacterial leaf stripe, one three major diseases affecting Sorghum bicolor. However, biochemical aspects pathophysiological host responses are not well understood. An untargeted metabolomics approach was designed to understand molecular mechanisms underlying S. bicolor⁻B. interactions. At 4-leaf stage, two sorghum cultivars (NS 5511 and NS 5655) differing in disease tolerance, were infected with B. metabolic changes monitored over time. The...

Plants have evolved both constitutive and inducible defence strategies to cope with different biotic stimuli stresses. Exposure of a plant challenging stress can lead primed state that allows it launch more rapid stronger defence. Here we applied metabolomic approach study compare the responses induced in Nicotiana tabacum cells by microbe-associated molecular pattern (MAMP) molecules, namely lipopolysaccharides (LPS), chitosan (CHT) flagellin-22 (FLG22). Early response metabolites,...