A5079286091- Fungal Plant Pathogen Control
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Plant pathogens and resistance mechanisms
- Wheat and Barley Genetics and Pathology
- Powdery Mildew Fungal Diseases
- Turfgrass Adaptation and Management
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Mycotoxins in Agriculture and Food
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogenic Bacteria Studies
- Biomedical Text Mining and Ontologies
- Phytoplasmas and Hemiptera pathogens
- Insect Resistance and Genetics
- Infectious Diseases and Mycology
- Wine Industry and Tourism
- Fermentation and Sensory Analysis
- Garlic and Onion Studies
- Weed Control and Herbicide Applications
- Advanced Text Analysis Techniques
- Glass properties and applications
- Nematode management and characterization studies
Curtin University
2016-2025
Rothamsted Research
2018
University of Cambridge
2018
Consejo Superior de Investigaciones Científicas
2006-2010
John Innes Centre
2010
Powdery mildews are phytopathogens whose growth and reproduction entirely dependent on living plant cells. The molecular basis of this life-style, obligate biotrophy, remains unknown. We present the genome analysis barley powdery mildew, Blumeria graminis f.sp. hordei (Blumeria), as well a comparison with two pathogenic dicotyledonous plants. These genomes display massive retrotransposon proliferation, genome-size expansion, gene losses. missing genes encode enzymes primary secondary...
Numerous vegetable crops are susceptible to powdery mildew, but cucurbits arguably the group most severely affected. Podosphaera fusca (synonym xanthii) is main causal agent of cucurbit mildew and one important limiting factors for production worldwide. Although great efforts have been invested in disease control, by contrast, many basic aspects biology P. remain unknown.Podosphaera (Fr.) Braun & Shishkoff. Kingdom Fungi; Phylum Ascomycota; Subdivision Pezizomycotina; Class Leotiomycetes;...
Evolved resistance to fungicides is a major problem limiting our ability control agricultural, medical and veterinary pathogens frequently associated with substitutions in the amino acid sequence of target protein. The convention for describing cite wild-type acid, codon number new using one-letter code. It has been observed that orthologous mutations have selected different species by from same mode action class, but acids numbers. These differences numbering arise lengths proteins each...
Pyrenophora teres f. sp. is the cause of net form blotch (NFNB), an economically important foliar disease in barley (Hordeum vulgare). Net and spot forms are widely controlled using site-specific systemic fungicides. Although resistance to succinate dehydrogenase inhibitors quinone outside has been addressed before blotches, mechanisms controlling demethylation inhibitor have not yet reported at molecular level. Here we report isolation strains NFNB Australia since 2013 resistant a range...
The dicarboximide fungicide iprodione has been used to combat blackleg disease of canola (Brassica napus), caused by the fungus Leptosphaeria maculans. For example, in Australia was late 1990s but is no longer registered for use against disease, and therefore impact on L. maculans not investigated. Resistance emerged spontaneously under vitro conditions at high frequency. A basis this resistance mutations hos1 gene that encodes a predicted osmosensing histidine kinase. While loss homologous...
Whether fungicide resistance management is optimized by spraying chemicals with different modes of action as a mixture (i.e., simultaneously) or in alternation sequentially) has been studied experimenters and modelers for decades. However, results have inconclusive. We use previously parameterized validated mathematical models wheat Septoria leaf blotch grapevine powdery mildew to test which tactic provides better management, using the total yield before causes disease control become...
Botrytis bunch rot, caused by cinerea, is an economically important disease of grapes in Australia and across grape-growing regions worldwide. Control this relies on canopy management the application fungicides. Fungicide can lead to selection resistant B. cinerea populations, which has adverse effect disease. Characterizing distribution severity populations needed inform resistance strategies.In study, 724 isolates were sampled from 76 Australian vineyards during 2013-2016 screened against...
Abstract Herbicides are the largest category of pesticides used in global agriculture, which is reflected rate increase number unique cases herbicide‐resistant weed biotypes since late 1950s. Recommended herbicide resistance management strategies and tactics have evolved over past 50 years through cumulative research experience been regularly reviewed. Nevertheless, new perspectives may be gained by viewing current recommended lens insecticide, fungicide, antibiotic management. What...
Decreased sensitivity to fungicides impacts the effectiveness of fungicide applications for managing plant disease. Knowledge frequency decreased in field populations is critical evaluating risks disease control. This study has applied a droplet digital PCR detection approach assess pathogen and quantify frequencies alleles associated with either demethylation inhibitor or succinate dehydrogenase Pyrenophora teres causing net blotch on barley Western Australia. f. maculata was most frequent...
Abstract Functionalized glass plays a crucial role in various fields, including materials and biomedical sciences. Traditionally, it has been produced through silanization reactions or by coating the with polymers. But these approaches involve toxic chemicals result films that are prone to hydrolysis upon long‐term exposure water. In this report, novel, simple method for functionalizing using ultrasonication of aryl diazonium salts is introduced. When exposed ultrasound under mild conditions...
Co-infections - invasions of a host-plant by multiple pathogen species or strains are common, and thought to have consequences for ecology evolution. Despite their apparent significance, co-infections received limited attention; in part due lack suitable quantitative tools monitoring co-infecting pathogens. Here, we report on duplex real-time PCR assay that simultaneously distinguishes quantifies two globally important fungal pathogens wheat: Pyrenophora tritici-repentis Parastagonospora...
Abstract Recessive mutations in the Mlo gene confer broad spectrum resistance barley ( Hordeum vulgare ) to powdery mildew Blumeria graminis f. sp. hordei ), a widespread and damaging disease. However, all alleles discovered date also display deleterious pleiotropic effects, including naturally occurring mlo-11 mutant which is widely deployed Europe. was Eth295, an Ethiopian landrace, developmentally controlled quantitative without spontaneous cell wall appositions or extensive necrosis loss...
Leptosphaeria maculans is the major pathogen of canola (oilseed rape, Brassica napus) worldwide. In Australia, use azole fungicides has contributed to 50-fold increase in production last 25 years. However, extensive application sets stage for selection fungal populations with resistance. A high-throughput planta assay was developed allow screening thousands isolates from multiple populations. Using this screen, were identified decreased sensitivity fungicide fluquinconazole when applied at...
The prevalence of azole resistance in Aspergillus fumigatus is uncertain Australia. Azole exposure may select for resistance. We investigated the frequency a large number clinical and environmental isolates.A. isolates [148 human, 21 animal 185 strains from air (n = 6) azole-exposed 64) or azole-naive 115) environments] were screened using VIPcheck™ system. MICs determined Sensititre™ YeastOne YO10 assay. Sequencing cyp51A gene promoter region was performed azole-resistant isolates, homology...
The fungal pathogen Pyrenophora teres f. sp. maculata (Ptm), responsible for spot-form of net blotch (SFNB), is currently the most significant disease barley in Australia and a major worldwide. Management SFNB relies heavily on fungicides demethylase inhibitors (DMIs) predominate. There have been sporadic reports resistance to DMIs Ptm but mechanisms remain obscure. isolates collected from 1996 2019 Western were tested fungicide sensitivity levels. Decreased was observed after 2015....
The increased occurrence of triazole fungicide resistant strains Blumeria graminis f. sp. hordei (Bgh) is an economic concern for the barley industry in Australia and elsewhere. High levels resistance to triazoles field are caused by two separate point mutations Cyp51 gene, Y136F S509T. Early detection these arising pathogen populations important as this allows time changes practices be adopted, thus mitigating potential yield losses due failure preventing from becoming dominant. A digital...
Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is a constant threat to barley production but generally well controlled through combinations of host genetics and fungicides. An epidemic powdery was observed from 2007 2013 in the West Australian grain belt.We collected isolates across Australia, examined their sensitivity demethylation inhibitor (DMI) fungicides sequenced Cyp51B target gene. Five amino acid substitutions were found, which four novel. The most resistant...
Septoria nodorum blotch (SNB) is a complex fungal disease of wheat caused by the Dothideomycete pathogen Parastagonospora nodorum. The fungus infects through use necrotrophic effectors (NEs) that cause necrosis on hosts carrying matching dominant susceptibility genes. Western Australia (WA) wheatbelt SNB "hot spot" and experiences as much 50% yield loss under favorable conditions. Consequently, has been major target for breeders in WA many years. In this study, we assembled panel 155 P....
As the incidence of fungicide resistance in plant pathogens continues to increase, control diseases and management would be greatly aided by rapid diagnostic methods. Quantitative allele-specific PCR (ASqPCR) is an ideal technique for in-field analysis as it can quantify frequency mutations targets. We have applied this fungal pathogen Blumeria graminis f. sp. tritici (Bgt), causal agent wheat powdery mildew. In Australia, strobilurin-resistant Bgt was first discovered 2016. Molecular...
Abstract BACKGROUND: Cucurbit powdery mildew elicited by Podosphaera fusca (Fr.) U Braun & N Shishkoff limits crop production in Spain. Disease control is largely dependent on fungicides such as sterol demethylation inhibitors (DMIs). Fungicide resistance an increasing problem this pathogen. To overcome risk, it necessary to design rational programmes based upon knowledge of field resistance. The aim study was investigate the state DMI sensitivity Spanish P. populations and provide tools...
Genome‐wide, unlinked, simple sequence repeat markers were used to examine genetic variation and relationships within Pyrenophora teres f. , a common pathogen of barley, in Western Australia. Despite the region's geographic isolation, isolates showed relatively high allelic compared similar studies, averaging 7.11 alleles per locus. Principal component, Bayesian clustering distance differentiation parameters provided evidence for both regional genotypic subdivision together with juxtaposing...
In a collection of 735 Botrytis isolates sampled from Australian wine grape‐growing regions, single isolate clade I and group (based on Bc‐hch RFLP analysis) was found. As many species are known to live sympatrically, it hypothesized that this might be new species. After phenotypic molecular assays supported hypothesis, the designated B. medusae . Phylogenetic analyses using nuclear genes G3 PDH , HSP 60 RPB 2 NEP 1 consistently placed in an early‐diverging spp. lineage. produced white...