A5042621025- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Orthopaedic implants and arthroplasty
- Electrospun Nanofibers in Biomedical Applications
- Quantum Dots Synthesis And Properties
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- biodegradable polymer synthesis and properties
- Bone fractures and treatments
- Nanoparticle-Based Drug Delivery
- Bone health and osteoporosis research
- Technology Adoption and User Behaviour
- Tissue Engineering and Regenerative Medicine
- Gold and Silver Nanoparticles Synthesis and Applications
- Consumer Retail Behavior Studies
- Collagen: Extraction and Characterization
- Spine and Intervertebral Disc Pathology
- Bone health and treatments
- Facial Trauma and Fracture Management
- Genetic and Environmental Crop Studies
- Carbon and Quantum Dots Applications
- Orthopedic Infections and Treatments
University of Kerala
1991-2025
University of Copenhagen
2024
Sree Chitra Thirunal Institute for Medical Sciences and Technology
2010-2020
United Arab Emirates University
2019
Institute of Medical Sciences
1993-2016
Goa Medical College
2014
Southern Methodist University
2011
Indian Institute of Technology Madras
2005
Rajiv Gandhi Centre for Biotechnology
2005
Kyoto University
2001
Gold nanoparticles of 20−100 nm diameter were synthesized within HEK-293 (human embryonic kidney), HeLa cervical cancer), SiHa and SKNSH neuroblastoma) cells. Incubation 1 mM tetrachloroaurate solution, prepared in phosphate buffered saline (PBS), pH 7.4, with human cells grown to ∼80% confluency yielded systematic growth over a period 96 h. The cells, stained due nanoparticle growth, adherent the bottom wells tissue culture plates, their morphology preserved, indicating that cell membrane...
The present work is a comparative evaluation of physical and biological properties electrospun biodegradable fibrous scaffolds based on polycaprolactone (PCL) its blend with polycaprolactone-polyethyleneglycol-polycaprolactone (CEC) without nanohydroxyapatite (nHAP) particles. fiber morphology, porosity, surface wettability, mechanical PCL were distinctly influenced by the presence both copolymer CEC nHAP. degradation in hydrolytic media affected morphological tensile strength decreased 58%...
The possibility of developing novel contrast imaging agents for cancer cellular labelling and fluorescence applications were explored using silica-coated cadmium selenide (CdSe) quantum dots (QDs). time dependent internalization efficiency study was carried out Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) Confocal Laser Scanning Microscopy (cLSM) after exposing QDs to stem cells cells. strong from the cytoplasm confirmed that efficiently internalized by maxima observed...
In the present study, we attempt to modify Polycaprolactone (PCL) by blending it with a water soluble polymer Polyethyleneoxide (PEO) having two different molecular weights (Mv ~1,00,000 and 6,00,000) using electrospinning technique. The effect of PEO weight blend ratio on fiber morphology, porosity, surface wettability, static dynamic mechanical properties PCL was investigated. vitro degradation studies in phosphate buffer saline (PBS) at 37 °C demonstrated formation pores especially...
ABSTRACT Platelet‐rich plasma (PRP) has been employed for orthopedic applications decades due to the abundance of bioactive cues/growth factors that ameliorate proliferation and migration relevant cells involved in tissue repair/regeneration. In this work, PRP was incorporated into injectable compositions alginate‐based hydrogel evaluated vitro vivo. tests revealed addition promoted cell adhesion, proliferation, distribution seeded fibrochondrocytes on hydrogel. Further, DNA quantification...
Surface reactivity of Calcium Phosphate materials--Hydroxyapatite (HA), Tricalcium (beta-TCP), Hydroxyapatite-Tricalcium (HA-TCP) were elucidated in a cell culture system. MG-63 osteoblast-like cells seeded onto the ceramic discs to evaluate changes morphology and functionality with respect different substrates. The dissolution re-precipitation calcium phosphate phases on surface medium was found be prominent beta-TCP when compared HA. Low (Ca), magnesium (Mg) alkaline phosphatase (ALP)...
New biotechnologies such as tissue engineering require functionally active cells within supportive matrices where the physical and chemical stimulus provided by matrix is indispensable to determine cellular behavior. This study has investigated influence of platelet-rich plasma (PRP) fibrin glue (FG) on functional activity goat bone marrow-derived mesenchymal stem (gBMSCs) that differentiated into osteogenic lineage. To achieve this goal, PRP FG were separately coated bioactive ceramics like...
Excessive demineralization in osteoporotic bones impairs its self-regeneration potential following a defect/fracture and is of great concern among the aged population. In this context, implants with inherent osteogenic ability loaded therapeutic ions like Strontium (Sr 2+ ) may bring forth promising outcomes. Micro-granular incorporated Hydroxyapatite scaffolds have been synthesized vivo efficacy was evaluated long-term osteoporosis-induced (LOA) rat model. Micro-granules improved surface...
Despite the success in use of superparamagnetic iron oxide nanoparticles (SPION) for various scientific applications, its potential biomedical fields has not been exploited to full potential. In this context, an situ substitution Mn(2+) was performed SPION and a series ferrite particles, MnxFe1-xFe2O4 with varying molar ratio : Fe(2+) where 'x' varies from 0-0.75. The particles obtained were further studied MRI contrast applications showed appreciable enhancement their properties. Manganese...
The central nervous system has limited regeneration potential. multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the neural tissues. However, likelihood their differentiation into unwanted lineages when transplanted a hostile injury environment is serious disadvantage. Transplanting predifferentiated via injectable carrier may aid in site-specific delivery better survival cells. Here, we focus on identifying appropriate hydrogel...
This paper is a trial to prepare collagen-hydroxyapatite composites in vitro by an alternate immersion method. Collagen sponges of different biodegradabilities were prepared through chemical cross-linking Type I collagen with glutaraldehyde (GA) at concentrations 0.2, 1.0, and 2.0 wt%. The immersed 37°C Tris-HCl-buffered solution containing 200 mM CaCl2 (pH 7.4) for 2 h then aqueous 120 Na2HPO4 9.3) further (one cycle). cycle was repeated times obtain composites. characterization the...
Abstract Hydroxyapatite (HA) ceramics are widely used as bone graft substitutes because of their biocompatibility and osteoconductivity. However, to enhance the success therapeutic application, many efforts undertaken improve bioactivity HA. We have developed a triphasic, silica‐containing ceramic‐coated hydroxyapatite (HASi) evaluated its performance scaffold for cell‐based tissue engineering applications. Human marrow stromal cells (hBMSCs) were seeded on both HASi HA scaffolds cultured...