A5041220789- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Diagnosis and treatment of tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Biochemical and Molecular Research
- Infectious Diseases and Tuberculosis
- Microbial infections and disease research
- Cancer therapeutics and mechanisms
- Inhalation and Respiratory Drug Delivery
- Immune responses and vaccinations
- Respiratory Support and Mechanisms
- Probiotics and Fermented Foods
- Cytomegalovirus and herpesvirus research
- Porphyrin Metabolism and Disorders
- Leprosy Research and Treatment
- Mechanical Circulatory Support Devices
- vaccines and immunoinformatics approaches
- Helicobacter pylori-related gastroenterology studies
- Infectious Diseases and Mycology
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Adenosine and Purinergic Signaling
- Antibiotic Resistance in Bacteria
- Pharmacogenetics and Drug Metabolism
Humboldt-Universität zu Berlin
2021-2025
Charité - Universitätsmedizin Berlin
2019-2025
Freie Universität Berlin
2021-2025
Max Planck Institute for Infection Biology
2016-2023
All India Institute of Medical Sciences
2007-2017
University of Cape Town
2013-2015
University of the Witwatersrand
2011
National Health Laboratory Service
2011
Aims: Polymerase chain reaction (PCR) is the most rapid and sensitive method for diagnosing mycobacterial infections identifying aetiological Mycobacterial species in order to administer appropriate therapy better patient management. Methods Results: Two hundred thirty‐five samples from 145 clinically suspected cases of tuberculosis were processed detection by ZN (Ziehl Neelsen) smear examination, L‐J & BACTECTM MGIT‐960 culture multiplex PCR tests. The comprised genus‐specific primers...
Vitamin B 12 -dependent enzymes function in core biochemical pathways Mycobacterium tuberculosis , an obligate pathogen whose metabolism vivo is poorly understood. Although M. can access vitamin vitro it uncertain whether the organism able to scavenge during host infection. This question crucial predictions of metabolic function, but its resolution complicated by absence genome a direct homologue BtuFCD, only bacterial transport system described date. We applied genome-wide transposon...
Tuberculosis is a major health problem in India, and the emergence of multidrug resistant (MDR) extensively drug (XDR) strains Mycobacterium tuberculosis (Mtb) has further complicated situation. Though several studies characterizing sensitive are available literature, almost all done on unrelated strains. Therefore, objective this study was to compare proteomic data four sequential isolates Mtb from single patient who developed MDR-TB during course anti-tuberculosis therapy (ATT).In study,...
Mycofactocin (MFT) belongs to the class of ribosomally synthesized and posttranslationally modified peptides conserved in many ActinobacteriaMycobacterium tuberculosis assimilates cholesterol during chronic infection, its vitro growth presence requires most MFT biosynthesis genes (mftA, mftB, mftC, mftD, mftE, mftF), although reasons for this requirement remain unclear. To identify function MFT, we characterized mutants constructed Mycobacterium smegmatis, M. marinum, We found that deficit...
Copper (Cu) ions are critical in controlling bacterial infections, and successful pathogens like Mycobacterium tuberculosis (Mtb) possess multiple Cu resistance mechanisms. We report, as proof of concept, that a novel hypersensitivity phenotype can be generated mycobacteria, including Mtb, through peptide, DAB-10, is able to form reactive oxygen species (ROS) following Cu-binding. DAB-10 induces intramycobacterial oxidative stress Cu-dependent manner vitro during infection. penetrates murine...
Nontuberculous mycobacteria are often underdiagnosed due to lack of proper diagnostic facilities. To overcome this, we created a rapid PCR method for the species-specific diagnosis Mycobacterium tuberculosis and its differentiation from other mycobacteria. A set primers targeting gene encoding early-secreted antigen-6 (ESAT-6) M. complex was designed standardized on mycobacterial standard strains 75 recent isolates AIDS patients 70 HIV-negative seen at hospital All India Institute Medical...
Fifty-four full-blown AIDS patients suspected of having HIV–tuberculosis co-infection were investigated for the prevalence extensively drug-resistant (XDR) Mycobacterium tuberculosis. Out 54 patients, M. tuberculosis was isolated from 24 (44.4%). Twelve (50%) isolates these had resistance to first-line drugs, whereas four (33.33%) also resistant second-line drugs. All in whom XDR isolated, died within 2.6 months diagnosis.
Human immune system mice are highly valuable for
ABSTRACT Lactate dehydrogenase A (LDHA) mediates interconversion of pyruvate and lactate, increased lactate turnover is exhibited by malignant infected immune cells. Hypoxic lung granuloma in Mycobacterium tuberculosis-infected animals present elevated levels Ldha lactate. Such alterations the metabolic milieu could influence outcome host-M. tuberculosis interactions. Given central role LDHA for tumorigenicity, targeting metabolism a promising approach cancer therapy. Here, we sought to...
Aim: This study was undertaken to compare the proteomic profile of sequential isolates Beijing lineage Mycobacterium tuberculosis (M. tuberculosis). from a patient who developed drug-resistant (TB) in vivo during anti-tuberculosis therapy (ATT). Background: Various studies have found M. strongly associated with multidrug resistance (MDR) development. Objectives: To identify and characterize differentially expressed proteins in-vivo drug conversion clinical isolates. Methods: Drug-susceptible...
BackgroundThe diagnosis of pulmonary tuberculosis (PTB) is conventionally established by examination three Ziehl–Neelsen stained smears; however, negative results do not preclude active TB. Since tubercle bacilli or their nucleic acids are also expected to be excreted through the kidneys, we assessed spot urine as a supplementary specimen for diagnosing PTB.MethodsA total 164 respiratory specimens (147 sputum, 15 bronchoalveolar lavage, and two gastric lavage) from 81 suspected PTB cases...
Background Delayed or missed diagnosis of TB continues to fuel the global epidemic, especially in resource limited settings. Use serology for tuberculosis, commonly used India, is another factor. In present study a commercially available serodiagnostic assay was assessed its diagnostic value combination with smear, culture and clinical manifestations. Methodology/Principal Findings A total 2300 subjects were recruited study, but 1041 excluded various reasons. Thus 1259 included which 470...
Abstract Improved methods are required for the early and accurate diagnosis of tuberculosis, especially in patients with smear-negative disease. Several biomarkers have been tried but most shown poor sensitivity or specificity. In present study we aimed to evaluate diagnostic utility five novel antigens identified earlier by us. This is an initial conducted on 250 subjects. The recombinant antigens, named as rSS1 (Rv2145c), rSS2 (Rv0164), rSS3 (Rv1437), rSS4 (Rv1827) rSS5 (Rv2970c), were...
Characterization of proteins with unknown functions is a critical research priority as the intracellular growth and metabolic state Mycobacterium tuberculosis , causative agent tuberculosis, remain poorly understood. Mycofactocin peptide-derived redox cofactor present in almost all mycobacterial species; however, its functional relevance M. pathogenesis host survival has never been studied experimentally.
Abstract Background Non-tubercular mycobacteria (NTM) has not been given due attention till the recent epidemic of HIV. This led to increasing interest health care workers in their biology, epidemiology and drug resistance. However, timely detection susceptibility profiling NTM isolates are always difficult resource poor settings like India. Furthermore, no standardized methodology or guidelines available reproduce results with clinical concordance. Objective To find an alternative rapid...
Respiratory infections caused by multidrug-resistant
ABSTRACT Mycobacterium avium subsp. paratuberculosis (MAP) is a well-established etiological agent of Johne’s disease in animals. In humans, similar clinical condition, first described by Crohn as regional ileitis 1932, now known Crohn’s diseases (CD), has also been associated with this mycobacterial species. However, there are two schools thoughts, one favoring MAP its while the second considers it an immune-inflammatory condition triggered external factor. Onset CD requires series events...