A5100352061- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Calcium signaling and nucleotide metabolism
- Retinal Development and Disorders
- Radiomics and Machine Learning in Medical Imaging
- MRI in cancer diagnosis
- Sirtuins and Resveratrol in Medicine
- Lysosomal Storage Disorders Research
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Genetic and Kidney Cyst Diseases
- Ion channel regulation and function
- Axon Guidance and Neuronal Signaling
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Electrochemical sensors and biosensors
- Lipid Membrane Structure and Behavior
- CRISPR and Genetic Engineering
Henan University
2024
Huazhong Agricultural University
2020-2024
Beijing Normal University
2024
State Key Laboratory of Cotton Biology
2024
Hebei Medical University
2023-2024
East China Normal University
2024
Shanghai Research Institute of Sports Science
2024
Viva Biotech (China)
2024
Xuzhou Medical College
2023
Sichuan University
2022-2023
Primary cilia, which emanate from the cell surface, exhibit assembly and disassembly dynamics along progression of cycle. However, mechanism that links ciliary cycle regulation remains elusive. In present study, we report Polo-like kinase 1 (Plk1), one key regulators, regulate centrosome maturation, bipolar spindle cytokinesis, acts as a pivotal player connects regulation. We found activity enriched Plk1 is required for primary cilia before mitotic entry, wherein interacts with activates...
Golgi reassembly stacking protein of 65 kDa (GRASP65) and 55 (GRASP55) were originally identified as proteins; however, subsequent GRASP knockdown experiments yielded inconsistent results with respect to the structure, indicating a limitation RNAi-based depletion. In this study, we have applied recently developed clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology knock out GRASP55 GRASP65, individually or in combination, HeLa HEK293 cells. We show that double...
Significance Age-associated degenerative diseases have similar pathogenic mechanisms related to defects in protein homeostasis. p97/VCP (valosin-containing protein) is essential for coordinating degradation and mutated a multisystem disease that affects the central nervous system, muscle, bone. an enzyme AAA ATPases (ATPases associated with diverse cellular activities) family, which takes apart ATP uses this energy perform pivotal functions. We found cofactors control its enzymatic activity....
Nedd1 is a new member of the γ-tubulin ring complex (γTuRC) and targets γTuRC to centrosomes for microtubule nucleation spindle assembly in mitosis. Although its role known, functional regulation mechanism remains unclear. Here we report that function regulated by Cdk1 Plk1. During mitosis, firstly phosphorylated at T550 Cdk1, which creates binding site polo-box domain Then, further Plk1 four sites: T382, S397, S637 S426. The sequential phosphorylation promotes interaction with targeting...
It has been indicated that the Golgi apparatus contributes to autophagy, but how it is involved in autophagosome formation and maturation not well understood. Here we show amino acid starvation causes trans-Golgi derived membrane fragments colocalize with autophagosomes. Depletion of stacking protein GORASP2/GRASP55, GORASP1/GRASP65, increases both MAP1LC3 (LC3)-II SQSTM1/p62 levels. We demonstrate GORASP2 facilitates autophagosome-lysosome fusion by physically linking autophagosomes...
The lysosomal degradation pathway, autophagy, is essential for the maintenance of cellular homeostasis. Recently, autophagy has been demonstrated to be required in process adipocyte conversion. However, its role mature adipocytes under physiological and pathological conditions remains unclear. Here, we report a major function BECN1 regulation basal adipocytes. We also show that berberine, natural plant alkaloid, inhibits adipose tissue mice fed high-fat diet via downregulation expression....
The purpose of this article is to investigate whether apparent diffusion coefficient (ADC) might be used as a universal biomarker for response evaluation in different tumors.Twenty-one patients with lung cancer, 12 esophageal 19 liver metastases, 24 gastric and 26 rectal cancer were recruited the study. Percentage changes ADC size responding nonresponding lesions tumors after treatment analyzed using repeated measures analysis variance.There was no significant difference among percentage (F...
In mammalian cells, autophagy plays crucial roles in restricting further spread of invading bacterial pathogens. Previous studies have established that the Salmonella virulence factors SseF and SseG are required for intracellular survival replication. However, underlying mechanism by which these two effectors facilitate infection remains elusive. Here, we report secreted Typhimurium (S. Typhimurium) inhibit host cells thereby establish a replicative niche bacteria cytosol. Mechanistically,...
In mammalian cells, the Golgi reassembly stacking protein 65 (GRASP65) has been implicated in both and ribbon linking by forming trans-oligomers through N-terminal GRASP domain. Because domain is globular relatively small, but gaps between stacks are large heterogeneous, it remains puzzling how GRASP65 physically links into a ribbon. To explore possibility that other proteins may help linking, we used biochemical methods identified actin elongation factor Mena as novel GRASP65-binding...
During the G2 to M phase transition, a portion of mitotic regulator Plk1 localizes kinetochores and regulates initiation kinetochore-microtubule attachments for proper chromosome alignment. Once attachment is achieved, this removed from as result ubiquitination. However, crucial molecular mechanism that promotes localization maintenance on until metaphase still unclear. We report ubiquitin-specific peptidase 16 (Usp16) plays key role during process. Usp16 deubiquitinates Plk1, resulting in...
It has been well documented that the ER responds to cellular stresses through unfolded protein response (UPR), but it is unknown how Golgi similar stresses. In this study, we treated HeLa cells with stress inducers, thapsigargin (TG), tunicamycin (Tm), and dithiothreitol (DTT), found only TG treatment resulted in fragmentation. induced fragmentation at a low dose short time when UPR was undetectable, indicating occurs independently of stress. Further experiments demonstrated induces...
The Database of Human Gastric Cancer (DBGC) is a comprehensive database that integrates various human gastric cancer-related data resources. transcriptomics projects, proteomics mutations, biomarkers and drug-sensitive genes from different sources were collected unified in this database. Moreover, epidemiological statistics cancer patients China clinicopathological information annotated with cases also integrated into the DBGC. We believe will greatly facilitate research regarding many...
In mammalian cells, the inheritance of Golgi apparatus into daughter cells during each cycle cell division is mediated by a disassembly and reassembly process, this process precisely controlled phosphorylation ubiquitination. VCIP135 (valosin-containing protein p97/p47 complex-interacting protein, p135), deubiquitinating enzyme required for p97/p47-mediated postmitotic membrane fusion, phosphorylated at multiple sites mitosis. However, whether directly regulates deubiquitinase activity...
The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins lipids. To perform these functions, forms multilayer stacked structure held by GRASP55 GRASP65 trans-oligomers perhaps their binding partners. Depletion GRASP disrupts stack formation impairs critical functions Golgi, such accurate protein glycosylation sorting. However, how destruction affects other cellular activities so far unknown. Here, we report depletion reduces cell...