A5007371167- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Biosensors and Analytical Detection
- Moringa oleifera research and applications
- Parasites and Host Interactions
- Insect Pest Control Strategies
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Pharmacological Effects of Medicinal Plants
- Synthesis and Biological Evaluation
- Genomics, phytochemicals, and oxidative stress
- Advanced biosensing and bioanalysis techniques
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
Centro Internacional de Entrenamiento e Investigaciones Medicas
2015-2025
Icesi University
2019-2025
We designed, developed, and clinically tested two rapid antigen-based immunosensors for SARS-CoV-2 detection, enabling diagnosis viral load quantification under USD $2. In a first clinical study, screen-printed disposable carbon-based (SPC) sensor was assessed on prospectively recruited adult participants classified into three study groups: healthy donors (n = 46); SARS-CoV-2-infected symptomatic patients 58); co-habitants of without prior testing 38). Nasopharyngeal aspirates (NA),...
Background The determinants of parasite persistence or elimination after treatment and clinical resolution cutaneous leishmaniasis (CL) are unknown. We investigated parasitological parameters associated with the presence viability Leishmania CL caused by L. Viannia. Methods Seventy patients who were treated meglumine antimoniate (n = 38) miltefosine 32) cured, included in this study. determined detection kDNA 7SLRNA transcripts, respectively, before, at end (EoT), 13 weeks initiation lesions...
Background The contribution of individuals with subclinical infection to the transmission and endemicity cutaneous leishmaniasis (CL) is unknown. Immunological evidence exposure Leishmania in residents endemic areas has been basis for defining human population asymptomatic infection. However, parasitological confirmation lacking. Methods We investigated presence viability blood non-invasive mucosal tissue samples from immunological CL caused by (Viannia) Colombia. Detection kDNA was...
The multifactorial basis of therapeutic response can obscure the relation between antimicrobial drug susceptibility and clinical outcome. To discern relationship parasite to meglumine antimoniate (SbV) outcome cutaneous leishmaniasis, risk factors for treatment failure were considered in evaluating this ninety-one leishmaniasis patients corresponding strains Leishmania ( Viannia ) panamensis . Parasite 32 μg SbV/mL (plasma C max was evaluated primary human macrophages, PBMCs, U937...
ABSTRACT. The endosymbiotic Leishmania RNA virus 1 (LRV1) has been associated with severity and clinical manifestations of American tegumentary leishmaniasis caused by species the ( Viannia ) subgenus. Between within species, among endemic countries, prevalence LRV is highly variable. not detected in L. V. panamensis , second-most prevalent Central America Colombia. However, no systematic screening conducted V .) thus it still controversial whether this truly absent from species. We sought...
The immune mechanisms that contribute to the efficacy of treatment cutaneous leishmaniasis (CL) are not fully understood. aim this study was define correlates outcome CL caused by Leishmania (Viannia) species during standard care with pentavalent antimonials. We conducted a comparative expression profiling response genes in peripheral blood mononuclear cells (PBMCs) and lesion biopsy specimens obtained from patients before at end (EoT) meglumine antimoniate. ex vivo PBMCs L (V) panamensis...
Abstract Background Colombia is ranked very high among countries with the highest numbers of endemic Leishmania species ( n = 9) causing human disease . Although much effort has been devoted to generating simple and specific tools for identification, challenges remain in discrimination belonging Viannia ) guyanensis complex: L. V. panamensis Methods A set seven reference strains subgenera, clinical from cases cutaneous leishmaniasis (CL; 26) samples collected sylvatic mammals sand flies 7)...
Early host-pathogen interactions drive the host response and shape outcome of natural infections caused by intracellular microorganisms. These involve a number immune non-immune cells tissues, along with an assortment pathogen-derived molecules. Our current knowledge has been predominantly derived from research on relationships between pathogens invaded cell(s), limiting our understanding how microbes elicit modulate immunological responses at organismal level. In this study, we explored...
Multiple polymerase chain reaction (PCR)-based approaches have been developed for Leishmania detection in clinical and laboratory samples, this diversity limits inter-study comparisons, meta-analyses, generalization of findings. Towards harmonization a molecular tool (Viannia) research purposes, we evaluated the concordance 18SrDNA quantitative (qPCR) minicircle kinetoplastid DNA (mkDNA) PCR followed by Southern blot (PCR-SB) vitro infection systems lesion mucosal swab samples from Colombian...
Failure of treatment cutaneous leishmaniasis with antimonial drugs and miltefosine is frequent. Use oral combination therapy represents an attractive strategy to increase efficacy reduce the risk drug resistance.
ABSTRACT Macrophages are the principal host cells of Leishmania spp . in human infection and play a critical role controlling enabling parasite survival persistence. Nevertheless, understanding drug resistance leishmaniasis has primarily focused on parasite. This investigation provides evidence significant differential macrophage response to ex vivo with clinical strains L. (V ) panamensis naturally resistant (zymodeme 2.3/zym 2.3) or sensitive 2.2/zym 2.2) antimonial drug, distinct effect...